Epigenetic variability in the genetically uniform forest tree species Pinus pinea L

Collada Collada, Maria Carmen and Sáez-laguna, Enrique and Guevara, M-a and Díaz, L-m and Sánchez-gómez, David and Aranda, Ismael and Cervera, M.t. (2014). Epigenetic variability in the genetically uniform forest tree species Pinus pinea L. "Plos One", v. 9 (n. 8); pp.. ISSN 1932-6203. https://doi.org/10.1371/journal.pone.0103145.

Description

Title: Epigenetic variability in the genetically uniform forest tree species Pinus pinea L
Author/s:
  • Collada Collada, Maria Carmen
  • Sáez-laguna, Enrique
  • Guevara, M-a
  • Díaz, L-m
  • Sánchez-gómez, David
  • Aranda, Ismael
  • Cervera, M.t.
Item Type: Article
Título de Revista/Publicación: Plos One
Date: 2014
ISSN: 1932-6203
Volume: 9
Subjects:
Faculty: E.T.S.I. Montes (UPM)
Department: Sistemas y Recursos Naturales
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

There is an increasing interest in understanding the role of epigenetic variability in forest species and how it may contribute to their rapid adaptation to changing environments. In this study we have conducted a genome-wide analysis of cytosine methylation pattern in Pinus pinea, a species characterized by very low levels of genetic variation and a remarkable degree of phenotypic plasticity. DNA methylation profiles of different vegetatively propagated trees from representative natural Spanish populations of P. pinea were analyzed with the Methylation Sensitive Amplified Polymorphism (MSAP) technique. A high degree of cytosine methylation was detected (64.36% of all scored DNA fragments). Furthermore, high levels of epigenetic variation were observed among the studied individuals. This high epigenetic variation found in P. pinea contrasted with the lack of genetic variation based on Amplified Fragment Length Polymorphism (AFLP) data. In this manner, variable epigenetic markers clearly discriminate individuals and differentiates two well represented populations while the lack of genetic variation revealed with the AFLP markers fail to differentiate at both, individual or population levels. In addition, the use of different replicated trees allowed identifying common polymorphic methylation sensitive MSAP markers among replicates of a given propagated tree. This set of MSAPs allowed discrimination of the 70% of the analyzed trees.

More information

Item ID: 35417
DC Identifier: http://oa.upm.es/35417/
OAI Identifier: oai:oa.upm.es:35417
DOI: 10.1371/journal.pone.0103145
Official URL: http://www.plosone.org
Deposited by: Memoria Investigacion
Deposited on: 28 May 2015 07:03
Last Modified: 08 May 2019 09:22
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