Evaluation of the bioaccumulation kinetics of gold nanorods in vital mammalian organs by mean of TXRF spectrometry

Fernández Ruiz, Ramón; Redrejo, María Jesús; Friedrich, Eberhardt Josue; Ramos Gomez, Milagros y Fernández Cabada, Tamara (2014). Evaluation of the bioaccumulation kinetics of gold nanorods in vital mammalian organs by mean of TXRF spectrometry. "Analytical Chemistry", v. 86 (n. 15); pp. 7383-7390. ISSN 0003-2700. https://doi.org/10.1021/ac5006475.

Descripción

Título: Evaluation of the bioaccumulation kinetics of gold nanorods in vital mammalian organs by mean of TXRF spectrometry
Autor/es:
  • Fernández Ruiz, Ramón
  • Redrejo, María Jesús
  • Friedrich, Eberhardt Josue
  • Ramos Gomez, Milagros
  • Fernández Cabada, Tamara
Tipo de Documento: Artículo
Título de Revista/Publicación: Analytical Chemistry
Fecha: Julio 2014
Volumen: 86
Materias:
Escuela: E.T.S.I. Telecomunicación (UPM)
Departamento: Tecnología Fotónica y Bioingeniería
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

This work presents the first application of total-reflection X-ray fluorescence (TXRF) spectrometry, a new and powerful alternative analytical method, to evaluation of the bioaccumulation kinetics of gold nanorods (GNRs) in various tissues upon intravenous administration in mice. The analytical parameters for developed methodology by TXRF were evaluated by means of the parallel analysis of bovine liver certified reference material samples (BCR-185R) doped with 10 μg/g gold. The average values (n = 5) achieved for gold measurements in lyophilized tissue weight were as follows: recovery 99.7%, expanded uncertainty (k = 2) 7%, repeatability 1.7%, detection limit 112 ng/g, and quantification limit 370 ng/g. The GNR bioaccumulation kinetics was analyzed in several vital mammalian organs such as liver, spleen, brain, and lung at different times. Additionally, urine samples were analyzed to study the kinetics of elimination of the GNRs by this excretion route. The main achievement was clearly differentiating two kinds of behaviors. GNRs were quickly bioaccumulated by highly vascular filtration organs such as liver and spleen, while GNRs do not show a bioaccumulation rates in brain and lung for the period of time investigated. In parallel, urine also shows a lack of GNR accumulation. TXRF has proven to be a powerful, versatile, and precise analytical technique for the evaluation of GNRs content in biological systems and, in a more general way, for any kind of metallic nanoparticles.

Más información

ID de Registro: 35623
Identificador DC: http://oa.upm.es/35623/
Identificador OAI: oai:oa.upm.es:35623
Identificador DOI: 10.1021/ac5006475
URL Oficial: http://pubs.acs.org/doi/abs/10.1021/ac5006475
Depositado por: Memoria Investigacion
Depositado el: 06 Jul 2015 16:10
Ultima Modificación: 06 Jul 2015 16:10
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