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Ballester Beltran, Jose and Lebourg, Myriam and Capella, Hector and Diaz Lantada, Andres and Salmeron Sanchez, Manuel (2014). Robust fabrication of electrospun-like polymer mats to direct cell bahaviour. "Biofabrication", v. 6 (n. 3); pp. 1-10. ISSN 1758-5082.
|Title:||Robust fabrication of electrospun-like polymer mats to direct cell bahaviour|
|Título de Revista/Publicación:||Biofabrication|
|Freetext Keywords:||Biophysical cues; Cell adhesion; Microfabrication; Cell alignment; Electrospinning; Myogenic differentiation; Poly(lactic acid)|
|Faculty:||E.T.S.I. Industriales (UPM)|
|Creative Commons Licenses:||Recognition - No derivative works - Non commercial|
Currently, cell culture systems that include nanoscale topography are widely used in order to provide cells additional cues closer to the in vivo environment, seeking to mimic the natural extracellular matrix. Electrospinning is one of the most common techniques to produce nano fiber mats. However, since many sensitive parameters play an important role in the process, a lack of reproducibility is a major drawback. Here we present a simple and robust methodology to prepare reproducible electrospun-like samples. It consists of a polydimethylsiloxane mold reproducing the fiber pattern to solvent-cast a polymer solution and obtain the final sample. To validate this methodology, poly(L-lactic) acid (PLLA) samples were obtained and, after characterisation, bioactivity and ability to direct cell response were assessed. C2C12 myoblasts developed focal adhesions on the electrospun-like fibers and, when cultured under myogenic differentiation conditions, similar differentiation levels to electrospun PLLA fibers were obtained.
|Deposited by:||Memoria Investigacion|
|Deposited on:||11 Mar 2016 08:47|
|Last Modified:||11 Mar 2016 08:47|