Robust fabrication of electrospun-like polymer mats to direct cell bahaviour

Ballester Beltran, Jose; Lebourg, Myriam; Capella, Hector; Diaz Lantada, Andres y Salmeron Sanchez, Manuel (2014). Robust fabrication of electrospun-like polymer mats to direct cell bahaviour. "Biofabrication", v. 6 (n. 3); pp. 1-10. ISSN 1758-5082. https://doi.org/10.1088/1758-5082/6/3/035009.

Descripción

Título: Robust fabrication of electrospun-like polymer mats to direct cell bahaviour
Autor/es:
  • Ballester Beltran, Jose
  • Lebourg, Myriam
  • Capella, Hector
  • Diaz Lantada, Andres
  • Salmeron Sanchez, Manuel
Tipo de Documento: Artículo
Título de Revista/Publicación: Biofabrication
Fecha: Mayo 2014
Volumen: 6
Materias:
Palabras Clave Informales: Biophysical cues; Cell adhesion; Microfabrication; Cell alignment; Electrospinning; Myogenic differentiation; Poly(lactic acid)
Escuela: E.T.S.I. Industriales (UPM)
Departamento: Ingeniería Mecánica
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Currently, cell culture systems that include nanoscale topography are widely used in order to provide cells additional cues closer to the in vivo environment, seeking to mimic the natural extracellular matrix. Electrospinning is one of the most common techniques to produce nano fiber mats. However, since many sensitive parameters play an important role in the process, a lack of reproducibility is a major drawback. Here we present a simple and robust methodology to prepare reproducible electrospun-like samples. It consists of a polydimethylsiloxane mold reproducing the fiber pattern to solvent-cast a polymer solution and obtain the final sample. To validate this methodology, poly(L-lactic) acid (PLLA) samples were obtained and, after characterisation, bioactivity and ability to direct cell response were assessed. C2C12 myoblasts developed focal adhesions on the electrospun-like fibers and, when cultured under myogenic differentiation conditions, similar differentiation levels to electrospun PLLA fibers were obtained.

Más información

ID de Registro: 35848
Identificador DC: http://oa.upm.es/35848/
Identificador OAI: oai:oa.upm.es:35848
Identificador DOI: 10.1088/1758-5082/6/3/035009
URL Oficial: http://iopscience.iop.org/article/10.1088/1758-5082/6/3/035009
Depositado por: Memoria Investigacion
Depositado el: 11 Mar 2016 08:47
Ultima Modificación: 11 Mar 2016 08:47
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