Arabidopsis DELLA and two HD-ZIP transcription factors regulate GA signalling in the epidermis through the L1 Box cis-element

Rombolá Caldentey, Belén and Rueda Romero, Paloma and Iglesias Fernandez, Raquel and Carbonero Zalduegui, Pilar and Oñate Sanchez, Luis (2014). Arabidopsis DELLA and two HD-ZIP transcription factors regulate GA signalling in the epidermis through the L1 Box cis-element. "Plant Cell", v. 26 (n. 7); pp. 2905-2919. ISSN 1040-4651. https://doi.org/10.1105/tpc.114.127647.

Description

Title: Arabidopsis DELLA and two HD-ZIP transcription factors regulate GA signalling in the epidermis through the L1 Box cis-element
Author/s:
  • Rombolá Caldentey, Belén
  • Rueda Romero, Paloma
  • Iglesias Fernandez, Raquel
  • Carbonero Zalduegui, Pilar
  • Oñate Sanchez, Luis
Item Type: Article
Título de Revista/Publicación: Plant Cell
Date: July 2014
ISSN: 1040-4651
Volume: 26
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Gibberellins (GAs) are plant hormones that affect plant growth and regulate gene expression differentially across tissues. To study the molecular mechanisms underlying GA signaling in Arabidopsis thaliana, we focused on a GDSL lipase gene (LIP1) induced by GA and repressed by DELLA proteins. LIP1 contains an L1 box promoter sequence, conserved in the promoters of epidermis-specific genes, that is bound by ATML1, an HD-ZIP transcription factor required for epidermis specification. In this study, we demonstrate that LIP1 is specifically expressed in the epidermis and that its L1 box sequence mediates GA-induced transcription. We show that this sequence is overrepresented in the upstream regulatory regions of GA-induced and DELLA-repressed transcriptomes and that blocking GA signaling in the epidermis represses the expression of L1 box–containing genes and negatively affects seed germination. We show that DELLA proteins interact directly with ATML1 and its paralogue PDF2 and that silencing of both HD-ZIP transcription factors inhibits epidermal gene expression and delays germination. Our results indicate that, upon seed imbibition, increased GA levels reduce DELLA protein abundance and release ATML1/PDF2 to activate L1 box gene expression, thus enhancing germination potential.

More information

Item ID: 35978
DC Identifier: http://oa.upm.es/35978/
OAI Identifier: oai:oa.upm.es:35978
DOI: 10.1105/tpc.114.127647
Official URL: http://www.plantcell.org/content/26/7/2905.abstract
Deposited by: Memoria Investigacion
Deposited on: 26 Jun 2015 15:28
Last Modified: 05 Nov 2018 13:45
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