The TRANSPLANTA collection of Arabidopsis lines: a resource for functional analysis of transcription factors based on their conditional over-expression

Coego, Alberto and Brizuela, Esther and Castillejo, Pablo and Ruíz, Sandra and Koncz, Csaba and Pozo, Juan C. del and Piñeiro Galvin, Manuel and Jarillo, José A. and Paz Ares, Javier and León, José (2014). The TRANSPLANTA collection of Arabidopsis lines: a resource for functional analysis of transcription factors based on their conditional over-expression. "The Plant Journal", v. 77 (n. 6); pp. 944-953. ISSN 0960-7412. https://doi.org/10.1111/tpj.12443.

Description

Title: The TRANSPLANTA collection of Arabidopsis lines: a resource for functional analysis of transcription factors based on their conditional over-expression
Author/s:
  • Coego, Alberto
  • Brizuela, Esther
  • Castillejo, Pablo
  • Ruíz, Sandra
  • Koncz, Csaba
  • Pozo, Juan C. del
  • Piñeiro Galvin, Manuel
  • Jarillo, José A.
  • Paz Ares, Javier
  • León, José
Item Type: Article
Título de Revista/Publicación: The Plant Journal
Date: March 2014
ISSN: 0960-7412
Volume: 77
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Transcription factors (TFs) are key regulators of gene expression in all organisms. In eukaryotes, TFs are often represented by functionally redundant members of large gene families. Overexpression might prove a means to unveil the biological functions of redundant TFs; however, constitutive overexpression of TFs frequently causes severe developmental defects, preventing their functional characterization. Conditional overexpression strategies help to overcome this problem. Here, we report on the TRANSPLANTA collection of Arabidopsis lines, each expressing one of 949 TFs under the control of a β–estradiol-inducible promoter. Thus far, 1636 independent homozygous lines, representing an average of 2.6 lines for every TF, have been produced for the inducible expression of 634 TFs. Along with a GUS-GFP reporter, randomly selected TRANSPLANTA lines were tested and confirmed for conditional transgene expression upon β–estradiol treatment. As a proof of concept for the exploitation of this resource, β–estradiol-induced proliferation of root hairs, dark-induced senescence, anthocyanin accumulation and dwarfism were observed in lines conditionally expressing full-length cDNAs encoding RHD6, WRKY22, MYB123/TT2 and MYB26, respectively, in agreement with previously reported phenotypes conferred by these TFs. Further screening performed with other TRANSPLANTA lines allowed the identification of TFs involved in different plant biological processes, illustrating that the collection is a powerful resource for the functional characterization of TFs. For instance, ANAC058 and a TINY/AP2 TF were identified as modulators of ABA-mediated germination potential, and RAP2.10/DEAR4 was identified as a regulator of cell death in the hypocotyl–root transition zone. Seeds of TRANSPLANTA lines have been deposited at the Nottingham Arabidopsis Stock Centre for further distribution.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainCSD2007‐00057CONSOLIDER TRANSPLANTAUnspecifiedUnspecified
Government of SpainBIO2011‐27526UnspecifiedUnspecifiedUnspecified
Government of SpainBIO2010‐15589UnspecifiedUnspecifiedUnspecified

More information

Item ID: 35979
DC Identifier: http://oa.upm.es/35979/
OAI Identifier: oai:oa.upm.es:35979
DOI: 10.1111/tpj.12443
Official URL: http://onlinelibrary.wiley.com/doi/10.1111/tpj.12443/abstract
Deposited by: Memoria Investigacion
Deposited on: 29 Jun 2015 14:01
Last Modified: 10 Jun 2019 08:01
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