Functional genomics tools to decipher the pathogenicity mechanisms of the necrotrophic fungus Plectosphaerella cucumerina in Arabidopsis thaliana

Ramos, Brisa and González Melendi, Pablo and Sánchez Vallet, Andrea and Sánchez Rodríguez, Clara and López García, Gemma and Molina Fernández, Antonio (2014). Functional genomics tools to decipher the pathogenicity mechanisms of the necrotrophic fungus Plectosphaerella cucumerina in Arabidopsis thaliana. "Molecular Plant Pathology", v. 14 (n. 1); pp. 44-57. ISSN 1464-6722. https://doi.org/10.1111/j.1364-3703.2012.00826.x.

Description

Title: Functional genomics tools to decipher the pathogenicity mechanisms of the necrotrophic fungus Plectosphaerella cucumerina in Arabidopsis thaliana
Author/s:
  • Ramos, Brisa
  • González Melendi, Pablo
  • Sánchez Vallet, Andrea
  • Sánchez Rodríguez, Clara
  • López García, Gemma
  • Molina Fernández, Antonio
Item Type: Article
Título de Revista/Publicación: Molecular Plant Pathology
Date: January 2014
ISSN: 1464-6722
Volume: 14
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The analysis of the interaction between Arabidopsis thaliana and adapted (PcBMM) and nonadapted (Pc2127) isolates of the necrotrophic fungus Plectosphaerella cucumerina has contributed to the identification of molecular mechanisms controlling plant resistance to necrotrophs.To characterize the pathogenicity bases of the virulence of necrotrophic fungi in Arabidopsis, we developed P. cucumerina functional genomics tools using Agrobacterium tumefaciens-mediated transformation.We generated PcBMM-GFP and Pc2127-GFP transformants constitutively expressing the green fluorescence protein (GFP), and a collection of random T-DNA insertional PcBMM transformants. Confocal microscopy analyses of the initial stages of PcBMM-GFP infection revealed that this pathogen, like other necrotrophic fungi, does not form an appressorium or penetrate into plant cells, but causes successive degradation of leaf cell layers

More information

Item ID: 36038
DC Identifier: http://oa.upm.es/36038/
OAI Identifier: oai:oa.upm.es:36038
DOI: 10.1111/j.1364-3703.2012.00826.x
Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1364-3703.2012.00826.x/abstract
Deposited by: Memoria Investigacion
Deposited on: 13 May 2016 14:56
Last Modified: 13 May 2016 14:56
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