Exploring new roles for the rpoS gene in the survival and virulence of the fire blight pathogen Erwinia amylovora

Santander, Ricardo D.; Monte Serrano, Mercedes; Rodríguez Herva, Jose Juan; Lopez Solanilla, Emilia; Rodriguez Palenzuela, Pablo y Biosca, Elena G. (2014). Exploring new roles for the rpoS gene in the survival and virulence of the fire blight pathogen Erwinia amylovora. "Fems Midrobiology Ecology", v. 90 (n. 3); pp. 895-907. ISSN 0168-6496. https://doi.org/10.1111/1574-6941.12444.

Descripción

Título: Exploring new roles for the rpoS gene in the survival and virulence of the fire blight pathogen Erwinia amylovora
Autor/es:
  • Santander, Ricardo D.
  • Monte Serrano, Mercedes
  • Rodríguez Herva, Jose Juan
  • Lopez Solanilla, Emilia
  • Rodriguez Palenzuela, Pablo
  • Biosca, Elena G.
Tipo de Documento: Artículo
Título de Revista/Publicación: Fems Midrobiology Ecology
Fecha: 2014
Volumen: 90
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnología - Biología Vegetal
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Erwinia amylovora causes fire blight in economically important plants of the family Rosaceae. This bacterial pathogen spends part of its life cycle coping with starvation and other fluctuating environmental conditions. In many Gram-negative bacteria, starvation and other stress responses are regulated by the sigma factor RpoS. We obtained an E. amylovora rpoS mutant to explore the role of this gene in starvation responses and its potential implication in other processes not yet studied in this pathogen. Results showed that E. amylovora needs rpoS to develop normal starvation survival and viable but nonculturable (VBNC) responses. Furthermore, this gene contributed to stationary phase cross-protection against oxidative, osmotic, and acid stresses and was essential for cross-protection against heat shock, but nonessential against acid shock. RpoS also mediated regulation of motility, exopolysaccharide synthesis, and virulence in immature loquats, but not in pear plantlets, and contributed to E. amylovora survival in nonhost tissues during incompatible interactions. Our results reveal some unique roles for the rpoS gene in E. amylovora and provide new knowledge on the regulation of different processes related to its ecology, including survival in different environments and virulence in immature fruits.

Más información

ID de Registro: 36039
Identificador DC: http://oa.upm.es/36039/
Identificador OAI: oai:oa.upm.es:36039
Identificador DOI: 10.1111/1574-6941.12444
URL Oficial: http://femsec.oxfordjournals.org/content/90/3/895
Depositado por: Memoria Investigacion
Depositado el: 01 Jul 2015 16:22
Ultima Modificación: 31 Ene 2016 23:56
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