Exploring new roles for the rpoS gene in the survival and virulence of the fire blight pathogen Erwinia amylovora

Santander, Ricardo D. and Monte Serrano, Mercedes and Rodríguez Herva, Jose Juan and Lopez Solanilla, Emilia and Rodriguez Palenzuela, Pablo and Biosca, Elena G. (2014). Exploring new roles for the rpoS gene in the survival and virulence of the fire blight pathogen Erwinia amylovora. "Fems Midrobiology Ecology", v. 90 (n. 3); pp. 895-907. ISSN 0168-6496. https://doi.org/10.1111/1574-6941.12444.

Description

Title: Exploring new roles for the rpoS gene in the survival and virulence of the fire blight pathogen Erwinia amylovora
Author/s:
  • Santander, Ricardo D.
  • Monte Serrano, Mercedes
  • Rodríguez Herva, Jose Juan
  • Lopez Solanilla, Emilia
  • Rodriguez Palenzuela, Pablo
  • Biosca, Elena G.
Item Type: Article
Título de Revista/Publicación: Fems Midrobiology Ecology
Date: 2014
ISSN: 0168-6496
Volume: 90
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Erwinia amylovora causes fire blight in economically important plants of the family Rosaceae. This bacterial pathogen spends part of its life cycle coping with starvation and other fluctuating environmental conditions. In many Gram-negative bacteria, starvation and other stress responses are regulated by the sigma factor RpoS. We obtained an E. amylovora rpoS mutant to explore the role of this gene in starvation responses and its potential implication in other processes not yet studied in this pathogen. Results showed that E. amylovora needs rpoS to develop normal starvation survival and viable but nonculturable (VBNC) responses. Furthermore, this gene contributed to stationary phase cross-protection against oxidative, osmotic, and acid stresses and was essential for cross-protection against heat shock, but nonessential against acid shock. RpoS also mediated regulation of motility, exopolysaccharide synthesis, and virulence in immature loquats, but not in pear plantlets, and contributed to E. amylovora survival in nonhost tissues during incompatible interactions. Our results reveal some unique roles for the rpoS gene in E. amylovora and provide new knowledge on the regulation of different processes related to its ecology, including survival in different environments and virulence in immature fruits.

More information

Item ID: 36039
DC Identifier: http://oa.upm.es/36039/
OAI Identifier: oai:oa.upm.es:36039
DOI: 10.1111/1574-6941.12444
Official URL: http://femsec.oxfordjournals.org/content/90/3/895
Deposited by: Memoria Investigacion
Deposited on: 01 Jul 2015 16:22
Last Modified: 31 Jan 2016 23:56
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