Molecular characterization of Glu-B3 locus in wheat cultivars and segregating populations

Espí Plaza, Araceli; Rodriguez De Quijano Urquiaga, Marta; Vazquez Muñiz, Jose Francisco; Carrillo Becerril, Jose Maria y Giraldo Carbajo, Patricia (2014). Molecular characterization of Glu-B3 locus in wheat cultivars and segregating populations. "Jounal of Cereal Science", v. 60 (n. 2); pp. 374-381. ISSN 0733-5210. https://doi.org/10.1016/j.jcs.2014.05.008.

Descripción

Título: Molecular characterization of Glu-B3 locus in wheat cultivars and segregating populations
Autor/es:
  • Espí Plaza, Araceli
  • Rodriguez De Quijano Urquiaga, Marta
  • Vazquez Muñiz, Jose Francisco
  • Carrillo Becerril, Jose Maria
  • Giraldo Carbajo, Patricia
Tipo de Documento: Artículo
Título de Revista/Publicación: Jounal of Cereal Science
Fecha: Septiembre 2014
Volumen: 60
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Bread wheat quality constitutes a key trait for the demands of the baking industry as well as the broad consumer preferences. The role of the low molecular weight glutenin subunits (LMW-GS) with regard to bread quality is so far not well understood owing to their genetic complexity and to the use of different nomenclatures and standards for the LMW-GS assignment by different research groups, which has made difficult the undertaking of association studies between genotypes and bread quality. The development of molecular markers to carry out genetic characterization and allele determination is demanding. Nowadays, the most promising LMW gene marker system is based on PCR and high resolution capillary electrophoresis for the simultaneous analysis of the complete multigene family. The molecular analysis of the bread wheat Glu-B3 locus in F2 and F4:6 populations expressed the expected one-locus Mendelian segregation pattern, thus validating the suitability of this marker system for the characterization of LMW-GS genes in segregating populations, allowing for the successful undertaking of studies related to bread-making quality. Moreover, the Glu-B3 allele characterization of standard cultivars with the molecular marker system has revealed its potential as a complementary tool for the allelic determination of this complex multigene family.

Más información

ID de Registro: 36101
Identificador DC: http://oa.upm.es/36101/
Identificador OAI: oai:oa.upm.es:36101
Identificador DOI: 10.1016/j.jcs.2014.05.008
URL Oficial: http://www.sciencedirect.com/science/article/pii/S0733521014001076
Depositado por: Memoria Investigacion
Depositado el: 01 Jul 2015 15:54
Ultima Modificación: 30 Sep 2015 22:56
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