Molecular characterization of Glu-B3 locus in wheat cultivars and segregating populations

Espí Plaza, Araceli and Rodriguez De Quijano Urquiaga, Marta and Vazquez Muñiz, Jose Francisco and Carrillo Becerril, Jose Maria and Giraldo Carbajo, Patricia (2014). Molecular characterization of Glu-B3 locus in wheat cultivars and segregating populations. "Jounal of Cereal Science", v. 60 (n. 2); pp. 374-381. ISSN 0733-5210. https://doi.org/10.1016/j.jcs.2014.05.008.

Description

Title: Molecular characterization of Glu-B3 locus in wheat cultivars and segregating populations
Author/s:
  • Espí Plaza, Araceli
  • Rodriguez De Quijano Urquiaga, Marta
  • Vazquez Muñiz, Jose Francisco
  • Carrillo Becerril, Jose Maria
  • Giraldo Carbajo, Patricia
Item Type: Article
Título de Revista/Publicación: Jounal of Cereal Science
Date: September 2014
ISSN: 0733-5210
Volume: 60
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Bread wheat quality constitutes a key trait for the demands of the baking industry as well as the broad consumer preferences. The role of the low molecular weight glutenin subunits (LMW-GS) with regard to bread quality is so far not well understood owing to their genetic complexity and to the use of different nomenclatures and standards for the LMW-GS assignment by different research groups, which has made difficult the undertaking of association studies between genotypes and bread quality. The development of molecular markers to carry out genetic characterization and allele determination is demanding. Nowadays, the most promising LMW gene marker system is based on PCR and high resolution capillary electrophoresis for the simultaneous analysis of the complete multigene family. The molecular analysis of the bread wheat Glu-B3 locus in F2 and F4:6 populations expressed the expected one-locus Mendelian segregation pattern, thus validating the suitability of this marker system for the characterization of LMW-GS genes in segregating populations, allowing for the successful undertaking of studies related to bread-making quality. Moreover, the Glu-B3 allele characterization of standard cultivars with the molecular marker system has revealed its potential as a complementary tool for the allelic determination of this complex multigene family.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGL2009-09980UnspecifiedUnspecifiedUnspecified
Government of SpainAGL2012-38345UnspecifiedUnspecifiedUnspecified

More information

Item ID: 36101
DC Identifier: http://oa.upm.es/36101/
OAI Identifier: oai:oa.upm.es:36101
DOI: 10.1016/j.jcs.2014.05.008
Official URL: http://www.sciencedirect.com/science/article/pii/S0733521014001076
Deposited by: Memoria Investigacion
Deposited on: 01 Jul 2015 15:54
Last Modified: 10 Jun 2019 10:05
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