Quantitative analysis of morphological changes in yeast colonies growing on solid medium: the eccentricity and Fourier indices

Gil de Prado, Elena and Rivas, Eva María and Siloniz, María Isabel and Diezma Iglesias, Belen and Barreiro Elorza, Pilar and Peinado, Jose Maria (2014). Quantitative analysis of morphological changes in yeast colonies growing on solid medium: the eccentricity and Fourier indices. "Yeast", v. 31 (n. 11); pp. 431-440. ISSN 1097-0061. https://doi.org/10.1002/yea.3036.

Description

Title: Quantitative analysis of morphological changes in yeast colonies growing on solid medium: the eccentricity and Fourier indices
Author/s:
  • Gil de Prado, Elena
  • Rivas, Eva María
  • Siloniz, María Isabel
  • Diezma Iglesias, Belen
  • Barreiro Elorza, Pilar
  • Peinado, Jose Maria
Item Type: Article
Título de Revista/Publicación: Yeast
Date: 14 September 2014
ISSN: 1097-0061
Volume: 31
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Ingeniería Agroforestal
UPM's Research Group: Técnicas Avanzadas en Agroalimentación LPF-TAGRALIA
Creative Commons Licenses: Recognition

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Abstract

The colony shape of four yeast species growing on agar medium wasmeasured for 116 days by image analysis. Initially, all the colonies are circular, with regular edges. The loss of circularity can be quantitatively estimated by the eccentricity index, Ei, calculated as the ratio between their orthogonal vertical and horizontal diameters. Ei can increase from 1 (complete circularity) to a maximum of 1.17–1.30, depending on the species. One colony inhibits its neighbour only when it has reached a threshold area. Then, Ei of the inhibited colony increases proportionally to the area of the inhibitory colony. The initial distance between colonies affects those threshold values but not the proportionality, Ei/area; this inhibition affects the shape but not the total surface of the colony. The appearance of irregularities in the edges is associated, in all the species, not with age but with nutrient exhaustion. The edge irregularity can be quantified by the Fourier index, Fi, calculated by the minimum number of Fourier coefficients that are needed to describe the colony contour with 99% fitness. An ad hoc function has been developed in Matlab v. 7.0 to automate the computation of the Fourier coefficients. In young colonies, Fi has a value between 2 (circumference) and 3 (ellipse). These values are maintained in mature colonies of Debaryomyces, but can reach values up to 14 in Saccharomyces.All the species studied showed the inhibition of growth in facing colony edges, but only three species showed edge irregularities associated with substrate exhaustion. Copyright © 2014 John Wiley & Sons, Ltd.

More information

Item ID: 37175
DC Identifier: http://oa.upm.es/37175/
OAI Identifier: oai:oa.upm.es:37175
DOI: 10.1002/yea.3036
Official URL: http://onlinelibrary.wiley.com/doi/10.1002/yea.3036/pdf
Deposited by: Catedrática Pilar Barreiro
Deposited on: 21 Jul 2015 06:48
Last Modified: 03 Nov 2016 17:15
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