A simple mathematical model that describes the growth of the area and the number of total and viable cells in yeast colonies

Rivas, Eva María; Gil de Prado, Elena; Wrent, P.; Sinoliz, Maria Isabel; Barreiro Elorza, Pilar; Correa Hernando, Eva Cristina; Conejero, F; Murciano, A y Peinado, Jose Maria (2014). A simple mathematical model that describes the growth of the area and the number of total and viable cells in yeast colonies. "Letters in applied microbiology", v. 59 (n. 6); pp. 594-603. ISSN 1472-765X. https://doi.org/10.1111/lam.12314.

Descripción

Título: A simple mathematical model that describes the growth of the area and the number of total and viable cells in yeast colonies
Autor/es:
  • Rivas, Eva María
  • Gil de Prado, Elena
  • Wrent, P.
  • Sinoliz, Maria Isabel
  • Barreiro Elorza, Pilar
  • Correa Hernando, Eva Cristina
  • Conejero, F
  • Murciano, A
  • Peinado, Jose Maria
Tipo de Documento: Artículo
Título de Revista/Publicación: Letters in applied microbiology
Fecha: Diciembre 2014
Volumen: 59
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Ingeniería Agroforestal
Grupo Investigación UPM: Técnicas Avanzadas en Agroalimentación LPF-TAGRALIA
Licencias Creative Commons: Reconocimiento

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Resumen

We propose a model, based on the Gompertz equation, to describe the growth of yeasts colonies on agar medium. This model presents several advantages: (i) one equation describes the colony growth, which previously needed two separate ones (linear increase of radius and of the squared radius); (ii) a similar equation can be applied to total and viable cells, colony area or colony radius, because the number of total cells in mature colonies is proportional to their area; and (iii) its parameters estimate the cell yield, the cell concentration that triggers growth limitation and the effect of this limitation on the specific growth rate. To elaborate the model, area, total and viable cells of 600 colonies of Saccharomyces cerevisiae, Debaryomyces fabryi, Zygosaccharomyces rouxii and Rhodotorula glutinis have been measured. With low inocula, viable cells showed an initial short exponential phase when colonies were not visible. This phase was shortened with higher inocula. In visible or mature colonies, cell growth displayed Gompertz-type kinetics. It was concluded that the cells growth in colonies is similar to liquid cultures only during the first hours, the rest of the time they grow, with near-zero specific growth rates, at least for 3 weeks.

Más información

ID de Registro: 37176
Identificador DC: http://oa.upm.es/37176/
Identificador OAI: oai:oa.upm.es:37176
Identificador DOI: 10.1111/lam.12314
URL Oficial: http://onlinelibrary.wiley.com/doi/10.1111/lam.12314/abstract
Depositado por: Catedrática Pilar Barreiro
Depositado el: 20 Jul 2015 12:11
Ultima Modificación: 01 Ene 2016 23:56
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