Whole-genome genotyping of grape using a panel of microsatellite

Zarouri, Belkacem and Vargas, Alba María and Gaforio, Laura and Aller, María and Andrés, María Teresa de and Cabezas, José Antonio (2015). Whole-genome genotyping of grape using a panel of microsatellite. "Tree Genetics & Genomes" ; pp. 17-32. ISSN 1614-2942. https://doi.org/10.1007/s11295-015-0843-4.

Description

Title: Whole-genome genotyping of grape using a panel of microsatellite
Author/s:
  • Zarouri, Belkacem
  • Vargas, Alba María
  • Gaforio, Laura
  • Aller, María
  • Andrés, María Teresa de
  • Cabezas, José Antonio
Item Type: Article
Título de Revista/Publicación: Tree Genetics & Genomes
Date: February 2015
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The use of microsatellite markers in large-scale genetic studies is limited by its low throughput and high cost and labor requirements. Here, we provide a panel of 45 multiplex PCRs for fast and cost-efficient genome-wide fluorescence-based microsatellite analysis in grapevine. The developed multiplex PCRs panel (with up to 15-plex) enables the scoring of 270 loci covering all the grapevine genome (9 to 20 loci/chromosome) using only 45 PCRs and sequencer runs. The 45 multiplex PCRs were validated using a diverse grapevine collection of 207 accessions, selected to represent most of the cultivated Vitis vinifera genetic diversity. Particular attention was paid to quality control throughout the whole process (assay replication, null allele detection, ease of scoring). Genetic diversity summary statistics and features of electrophoretic profiles for each studied marker are provided, as are the genotypes of 25 common cultivars that could be used as references in other studies.

More information

Item ID: 37978
DC Identifier: http://oa.upm.es/37978/
OAI Identifier: oai:oa.upm.es:37978
DOI: 10.1007/s11295-015-0843-4
Official URL: http://link.springer.com/article/10.1007/s11295-015-0843-4
Deposited by: Memoria Investigacion
Deposited on: 05 Nov 2015 13:50
Last Modified: 30 Apr 2016 22:56
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