Improved walnut mass micropropagation through the combined use of phloroglucinol and FeEDDHA

Licea Moreno, Ricardo Julián; Contreras Mogollón, Ángela Bibiana; Morales, Ana V.; Urban, Ignacio; Daquinta, Marcos y Gomez Fernandez, Luis (2015). Improved walnut mass micropropagation through the combined use of phloroglucinol and FeEDDHA. "Plant Cell Tissue And Organ Culture", v. 123 (n. 1); pp. 143-154. ISSN 0167-6857.

Descripción

Título: Improved walnut mass micropropagation through the combined use of phloroglucinol and FeEDDHA
Autor/es:
  • Licea Moreno, Ricardo Julián
  • Contreras Mogollón, Ángela Bibiana
  • Morales, Ana V.
  • Urban, Ignacio
  • Daquinta, Marcos
  • Gomez Fernandez, Luis
Tipo de Documento: Artículo
Título de Revista/Publicación: Plant Cell Tissue And Organ Culture
Fecha: Octubre 2015
Volumen: 123
Materias:
Escuela: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Despite the socioeconomic importance of walnut trees, poor rooting and recalcitrance to in vitro culture have hampered the establishment of high-yield clonal plantations. To improve walnut micropropagation, we introduced several modifications to current methods and evaluated the effects on microshoot performance and acclimatization. Nine selected genotypes (13-year-old trees) of the commercial hybrid Juglans major 209 x J. regia were cultured in vitro on DKW-C medium supplemented with 4.4 µM BA and 50 µM IBA. A protocol was developed that relies on the use of 0.40 mM phloroglucinol during shoot multiplication, 0.20 mM previous root induction, and 6.81 mg/L Fe3+ (FeEDDHA). Moreover, the addition of 83.2 µM glucose during the root expression phase significantly improved plant survival during acclimatization. Phloroglucinol promoted microshoot elongation but inhibited rooting, especially at concentrations above 0.40 mM. Replacing FeEDTA by FeEDDHA diminished chlorotic symptoms and improved rooting, with up to 90% microshoots developing viable roots. Likewise, glucose was more efficient than sucrose or fructose in promoting plant survival. At the proposed working concentrations, neither glucose nor FeEDDHA caused any noticeable deleterious effect on walnut micropropagation. Microscopic analysis revealed the physical continuity between adventitious roots and stem pericycles. Analysis of leaf genomic DNA with eight polymorphic microsatellite markers was supportive of the clonal fidelity and genetic stability of the micropropagated material. Successful clonal plantations (over 5,800 ramets) have been established by applying this protocol.

Más información

ID de Registro: 38190
Identificador DC: http://oa.upm.es/38190/
Identificador OAI: oai:oa.upm.es:38190
URL Oficial: http://link.springer.com/article/10.1007/s11240-015-0822-3
Depositado por: Memoria Investigacion
Depositado el: 13 May 2016 14:42
Ultima Modificación: 13 May 2016 14:42
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