Improved walnut mass micropropagation through the combined use of phloroglucinol and FeEDDHA

Licea Moreno, Ricardo Julián and Contreras Mogollón, Ángela Bibiana and Morales, Ana V. and Urban, Ignacio and Daquinta, Marcos and Gomez Fernandez, Luis (2015). Improved walnut mass micropropagation through the combined use of phloroglucinol and FeEDDHA. "Plant Cell Tissue And Organ Culture", v. 123 (n. 1); pp. 143-154. ISSN 0167-6857. https://doi.org/10.1007/s11240-015-0822-3.

Description

Title: Improved walnut mass micropropagation through the combined use of phloroglucinol and FeEDDHA
Author/s:
  • Licea Moreno, Ricardo Julián
  • Contreras Mogollón, Ángela Bibiana
  • Morales, Ana V.
  • Urban, Ignacio
  • Daquinta, Marcos
  • Gomez Fernandez, Luis
Item Type: Article
Título de Revista/Publicación: Plant Cell Tissue And Organ Culture
Date: October 2015
ISSN: 0167-6857
Volume: 123
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Despite the socioeconomic importance of walnut trees, poor rooting and recalcitrance to in vitro culture have hampered the establishment of high-yield clonal plantations. To improve walnut micropropagation, we introduced several modifications to current methods and evaluated the effects on microshoot performance and acclimatization. Nine selected genotypes (13-year-old trees) of the commercial hybrid Juglans major 209 x J. regia were cultured in vitro on DKW-C medium supplemented with 4.4 µM BA and 50 µM IBA. A protocol was developed that relies on the use of 0.40 mM phloroglucinol during shoot multiplication, 0.20 mM previous root induction, and 6.81 mg/L Fe3+ (FeEDDHA). Moreover, the addition of 83.2 µM glucose during the root expression phase significantly improved plant survival during acclimatization. Phloroglucinol promoted microshoot elongation but inhibited rooting, especially at concentrations above 0.40 mM. Replacing FeEDTA by FeEDDHA diminished chlorotic symptoms and improved rooting, with up to 90% microshoots developing viable roots. Likewise, glucose was more efficient than sucrose or fructose in promoting plant survival. At the proposed working concentrations, neither glucose nor FeEDDHA caused any noticeable deleterious effect on walnut micropropagation. Microscopic analysis revealed the physical continuity between adventitious roots and stem pericycles. Analysis of leaf genomic DNA with eight polymorphic microsatellite markers was supportive of the clonal fidelity and genetic stability of the micropropagated material. Successful clonal plantations (over 5,800 ramets) have been established by applying this protocol.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGL2007–64761/FORUnspecifiedUnspecifiedUnspecified

More information

Item ID: 38190
DC Identifier: http://oa.upm.es/38190/
OAI Identifier: oai:oa.upm.es:38190
DOI: 10.1007/s11240-015-0822-3
Official URL: http://link.springer.com/article/10.1007/s11240-015-0822-3
Deposited by: Memoria Investigacion
Deposited on: 13 May 2016 14:42
Last Modified: 03 Jun 2019 14:48
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