Optimization of Dengue immunoassay by label-free interferometric optical detection method

Laguna Heras, Maria Fe and Holgado Bolaños, Miguel and Sanza Gutiérrez, Francisco Javier and Lavin Hueros, Alvaro and López Hernández, Ana and Casquel del Campo, Rafael (2014). Optimization of Dengue immunoassay by label-free interferometric optical detection method. "Sensors", v. 14 ; pp. 6695-6700. ISSN 1424-8220. https://doi.org/10.3390/s140406695.

Description

Title: Optimization of Dengue immunoassay by label-free interferometric optical detection method
Author/s:
  • Laguna Heras, Maria Fe
  • Holgado Bolaños, Miguel
  • Sanza Gutiérrez, Francisco Javier
  • Lavin Hueros, Alvaro
  • López Hernández, Ana
  • Casquel del Campo, Rafael
Item Type: Article
Título de Revista/Publicación: Sensors
Date: April 2014
ISSN: 1424-8220
Volume: 14
Subjects:
Freetext Keywords: photonic sensing cells; biosensors; label-free immunoassay; dengue virus
Faculty: E.T.S.I. Industriales (UPM)
Department: Física Aplicada e Ingeniería de Materiales
UPM's Research Group: Group of Micro-nano Photonics and Biophotonics, Laser Center, and Center for Biomedical Technology
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

In this communication we report a direct immunoassay for detecting dengue virus by means of a label-free interferometric optical detection method. We also demonstrate how we can optimize this sensing response by adding a blocking step able to significantly enhance the optical sensing response. The blocking reagent used for this optimization is a dry milk diluted in phosphate buffered saline. The recognition curve of dengue virus over the proposed surface sensor demonstrates the capacity of this method to be applied in Point of Care technology.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainIPT-2011-1429-010000UnspecifiedUnspecifiedProject INNBIOD
Government of SpainTEC20121-31145UnspecifiedUnspecifiedProject PLATON
FP7FP7 - OCEAN - 2013UnspecifiedUnspecifiedENVIGUARD EUROPEAN PROJECT

More information

Item ID: 40118
DC Identifier: http://oa.upm.es/40118/
OAI Identifier: oai:oa.upm.es:40118
DOI: 10.3390/s140406695
Official URL: http://www.mdpi.com/journal/sensors
Deposited by: Memoria Investigacion
Deposited on: 05 May 2016 15:21
Last Modified: 05 May 2016 15:21
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