Effect of epidermal growth factor on nuclear and cytoplasmic in vitro maruration of guinea pig oocytes

Cañón Beltrán, K. and Cajas, Y.N. and García García, R. M. and Lorenzo, P.L. and Carrera, R. and Garcia Rebollar, Pilar and Arias Álvarez, María (2015). Effect of epidermal growth factor on nuclear and cytoplasmic in vitro maruration of guinea pig oocytes. "Animal Reproduction", v. 12 (n. 3); p. 793. ISSN 1806-9614.

Description

Title: Effect of epidermal growth factor on nuclear and cytoplasmic in vitro maruration of guinea pig oocytes
Author/s:
  • Cañón Beltrán, K.
  • Cajas, Y.N.
  • García García, R. M.
  • Lorenzo, P.L.
  • Carrera, R.
  • Garcia Rebollar, Pilar
  • Arias Álvarez, María
Item Type: Article
Título de Revista/Publicación: Animal Reproduction
Date: 2015
ISSN: 1806-9614
Volume: 12
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Producción Agraria
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The guinea pig may represent an animal model for research on ovarian infertility and improvement of the in vitro maturation (IVM) conditions is needed in this species. The aim of the present work was to immunolocalize the Epidermal Growth Factor (EGF)-Receptor in the guinea pig ovaries and to study the effect of EGF on meiotic and cytoplasmic maturation, and apoptotic rate in cumulus-oocyte-co mplexes (COCs). Immunohistochemistry was performed in paraffined ovaries using a rabbit polyclonal antibody EGF-R (1:100; Santa Cruz Biotechnology) and the ABC Vector Elite kit (Vector Laboratories). For the IVM, COCs were collected by aspiration of follicles >700μm under a stereoscopic microscope.

More information

Item ID: 40169
DC Identifier: http://oa.upm.es/40169/
OAI Identifier: oai:oa.upm.es:40169
Deposited by: Memoria Investigacion
Deposited on: 05 May 2016 15:02
Last Modified: 04 Jun 2019 15:17
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