Mannans and endo-beta-mannanases (MAN) in Brachypodium distachyon: expression profiling and possible role of the BdMAN genes during coleorhiza-limited seed germination

González de la Calle, Virginia; Barrero Sicilia, Cristina; Carbonero Zalduegui, Pilar y Iglesias Fernandez, Raquel (2015). Mannans and endo-beta-mannanases (MAN) in Brachypodium distachyon: expression profiling and possible role of the BdMAN genes during coleorhiza-limited seed germination. "Journal of Experimental Botany", v. 66 (n. 13); pp. 3753-3764. ISSN 0022-0957. https://doi.org/10.1093/jxb/erv168.

Descripción

Título: Mannans and endo-beta-mannanases (MAN) in Brachypodium distachyon: expression profiling and possible role of the BdMAN genes during coleorhiza-limited seed germination
Autor/es:
  • González de la Calle, Virginia
  • Barrero Sicilia, Cristina
  • Carbonero Zalduegui, Pilar
  • Iglesias Fernandez, Raquel
Tipo de Documento: Artículo
Título de Revista/Publicación: Journal of Experimental Botany
Fecha: 2015
Volumen: 66
Materias:
Escuela: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Departamento: Otro
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Immunolocalization of mannans in the seeds of Brachypodium distachyon reveals the presence of these polysaccharides in the root embryo and in the coleorhiza in the early stages of germination (12h), decreasing thereafter to the point of being hardly detected at 27h. Concurrently, the activity of endo-β-mannanases (MANs; EC 3.2.1.78) that catalyse the hydrolysis of β-1,4 bonds in mannan polymers, increases as germination progresses. The MAN gene family is represented by six members in the Brachypodium genome, and their expression has been explored in different organs and especially in germinating seeds. Transcripts of BdMAN2, BdMAN4 and BdMAN6 accumulate in embryos, with a maximum at 24–30h, and are detected in the coleorhiza and in the root by in situ hybridization analyses, before root protrusion (germination sensu stricto). BdMAN4 is not only present in the embryo root and coleorhiza, but is abundant in the de-embryonated (endosperm) imbibed seeds, while BdMAN2 and BdMAN6 are faintly expressed in endosperm during post-germination (36–42h). BdMAN4 and BdMAN6 transcripts are detected in the aleurone layer. These data indicate that BdMAN2, BdMAN4 and BdMAN6 are important for germination sensu stricto and that BdMAN4 and BdMAN6 may also influence reserve mobilization. Whether the coleorhiza in monocots and the micropylar endosperm in eudicots have similar functions, is discussed.

Más información

ID de Registro: 40461
Identificador DC: http://oa.upm.es/40461/
Identificador OAI: oai:oa.upm.es:40461
Identificador DOI: 10.1093/jxb/erv168
URL Oficial: http://jxb.oxfordjournals.org/content/66/13/3753.abstract
Depositado por: Memoria Investigacion
Depositado el: 17 May 2016 15:55
Ultima Modificación: 17 May 2016 15:55
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