Mannans and endo-beta-mannanases (MAN) in Brachypodium distachyon: expression profiling and possible role of the BdMAN genes during coleorhiza-limited seed germination

González de la Calle, Virginia and Barrero Sicilia, Cristina and Carbonero Zalduegui, Pilar and Iglesias Fernandez, Raquel (2015). Mannans and endo-beta-mannanases (MAN) in Brachypodium distachyon: expression profiling and possible role of the BdMAN genes during coleorhiza-limited seed germination. "Journal of Experimental Botany", v. 66 (n. 13); pp. 3753-3764. ISSN 0022-0957. https://doi.org/10.1093/jxb/erv168.

Description

Title: Mannans and endo-beta-mannanases (MAN) in Brachypodium distachyon: expression profiling and possible role of the BdMAN genes during coleorhiza-limited seed germination
Author/s:
  • González de la Calle, Virginia
  • Barrero Sicilia, Cristina
  • Carbonero Zalduegui, Pilar
  • Iglesias Fernandez, Raquel
Item Type: Article
Título de Revista/Publicación: Journal of Experimental Botany
Date: 2015
ISSN: 0022-0957
Volume: 66
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Immunolocalization of mannans in the seeds of Brachypodium distachyon reveals the presence of these polysaccharides in the root embryo and in the coleorhiza in the early stages of germination (12h), decreasing thereafter to the point of being hardly detected at 27h. Concurrently, the activity of endo-β-mannanases (MANs; EC 3.2.1.78) that catalyse the hydrolysis of β-1,4 bonds in mannan polymers, increases as germination progresses. The MAN gene family is represented by six members in the Brachypodium genome, and their expression has been explored in different organs and especially in germinating seeds. Transcripts of BdMAN2, BdMAN4 and BdMAN6 accumulate in embryos, with a maximum at 24–30h, and are detected in the coleorhiza and in the root by in situ hybridization analyses, before root protrusion (germination sensu stricto). BdMAN4 is not only present in the embryo root and coleorhiza, but is abundant in the de-embryonated (endosperm) imbibed seeds, while BdMAN2 and BdMAN6 are faintly expressed in endosperm during post-germination (36–42h). BdMAN4 and BdMAN6 transcripts are detected in the aleurone layer. These data indicate that BdMAN2, BdMAN4 and BdMAN6 are important for germination sensu stricto and that BdMAN4 and BdMAN6 may also influence reserve mobilization. Whether the coleorhiza in monocots and the micropylar endosperm in eudicots have similar functions, is discussed.

More information

Item ID: 40461
DC Identifier: http://oa.upm.es/40461/
OAI Identifier: oai:oa.upm.es:40461
DOI: 10.1093/jxb/erv168
Official URL: http://jxb.oxfordjournals.org/content/66/13/3753.abstract
Deposited by: Memoria Investigacion
Deposited on: 17 May 2016 15:55
Last Modified: 17 May 2016 15:55
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