Medicago truncatula Natural Resistance Associated Macrophage Protein1 is required for iron uptake by rhizobia infected nodule cells

Tejada Jiménez, Manuel; Castro Rodríguez, Rosario; Kryvoruchko, Igor; Lucas, M. Mercedes; Udvardi, Michael; Imperial Ródenas, Juan y Gonzalez Guerrero, Manuel (2015). Medicago truncatula Natural Resistance Associated Macrophage Protein1 is required for iron uptake by rhizobia infected nodule cells. "Plant Physiol", v. 168 (n. 1); pp. 258-272. ISSN 0032-0889. https://doi.org/10.​1104/​pp.​114.​254672.

Descripción

Título: Medicago truncatula Natural Resistance Associated Macrophage Protein1 is required for iron uptake by rhizobia infected nodule cells
Autor/es:
  • Tejada Jiménez, Manuel
  • Castro Rodríguez, Rosario
  • Kryvoruchko, Igor
  • Lucas, M. Mercedes
  • Udvardi, Michael
  • Imperial Ródenas, Juan
  • Gonzalez Guerrero, Manuel
Tipo de Documento: Artículo
Título de Revista/Publicación: Plant Physiol
Fecha: Mayo 2015
Volumen: 168
Materias:
Escuela: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Departamento: Otro
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Iron is critical for symbiotic nitrogen fixation (SNF) as a key component ofmultiple ferroproteins involved in this biological process. In the model legume Medicago truncatula, iron is delivered by the vasculature to the infection/maturation zone (zone II) of the nodule, where it is released to the apoplast. From there, plasma membrane iron transporters move it into rhizobia-containing cells, where iron is used as the cofactor of multiple plant and rhizobial proteins (e.g. plant leghemoglobin and bacterial nitrogenase). MtNramp1 (Medtr3g088460) is the M. truncatula Natural Resistance-Associated Macrophage Protein family member, with the highest expression levels in roots and nodules. Immunolocalization studies indicate that MtNramp1 is mainly targeted to the plasma membrane. A loss-of-function nramp1 mutant exhibited reduced growth compared with the wild type under symbiotic conditions, but not when fertilized with mineral nitrogen. Nitrogenase activity was low in the mutant, whereas exogenous iron and expression of wild-type MtNramp1 in mutant nodules increased nitrogen fixation to normal levels. These data are consistent with a model in which MtNramp1 is the main transporter responsible for apoplastic iron uptake by rhizobia-infected cells in zone II.

Proyectos asociados

TipoCódigoAcrónimoResponsableTítulo
FP7ERC-2013-StG-335284METALSYMUNIVERSIDAD POLITECNICA DE MADRIDMetal transport in the tripartite symbiosis arbuscular mycorrhizal fungi-legume-rhizobia
FP7IRG-2010-276771MENOMEDUNIVERSIDAD POLITECNICA DE MADRIDMetal homeostasis in nodulated Medicago plants
Gobierno de EspañaAGL-2012-32974Sin especificarSin especificarSin especificar

Más información

ID de Registro: 40465
Identificador DC: http://oa.upm.es/40465/
Identificador OAI: oai:oa.upm.es:40465
Identificador DOI: 10.​1104/​pp.​114.​254672
URL Oficial: http://www.plantphysiol.org/content/168/1/258.abstract
Depositado por: Memoria Investigacion
Depositado el: 17 May 2016 16:01
Ultima Modificación: 17 May 2016 16:01
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