Medicago truncatula Natural Resistance Associated Macrophage Protein1 is required for iron uptake by rhizobia infected nodule cells

Tejada Jiménez, Manuel and Castro Rodríguez, Rosario and Kryvoruchko, Igor and Lucas, M. Mercedes and Udvardi, Michael and Imperial Ródenas, Juan and Gonzalez Guerrero, Manuel (2015). Medicago truncatula Natural Resistance Associated Macrophage Protein1 is required for iron uptake by rhizobia infected nodule cells. "Plant Physiol", v. 168 (n. 1); pp. 258-272. ISSN 0032-0889. https://doi.org/10.1104/pp.114.254672.

Description

Title: Medicago truncatula Natural Resistance Associated Macrophage Protein1 is required for iron uptake by rhizobia infected nodule cells
Author/s:
  • Tejada Jiménez, Manuel
  • Castro Rodríguez, Rosario
  • Kryvoruchko, Igor
  • Lucas, M. Mercedes
  • Udvardi, Michael
  • Imperial Ródenas, Juan
  • Gonzalez Guerrero, Manuel
Item Type: Article
Título de Revista/Publicación: Plant Physiol
Date: May 2015
ISSN: 0032-0889
Volume: 168
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Iron is critical for symbiotic nitrogen fixation (SNF) as a key component ofmultiple ferroproteins involved in this biological process. In the model legume Medicago truncatula, iron is delivered by the vasculature to the infection/maturation zone (zone II) of the nodule, where it is released to the apoplast. From there, plasma membrane iron transporters move it into rhizobia-containing cells, where iron is used as the cofactor of multiple plant and rhizobial proteins (e.g. plant leghemoglobin and bacterial nitrogenase). MtNramp1 (Medtr3g088460) is the M. truncatula Natural Resistance-Associated Macrophage Protein family member, with the highest expression levels in roots and nodules. Immunolocalization studies indicate that MtNramp1 is mainly targeted to the plasma membrane. A loss-of-function nramp1 mutant exhibited reduced growth compared with the wild type under symbiotic conditions, but not when fertilized with mineral nitrogen. Nitrogenase activity was low in the mutant, whereas exogenous iron and expression of wild-type MtNramp1 in mutant nodules increased nitrogen fixation to normal levels. These data are consistent with a model in which MtNramp1 is the main transporter responsible for apoplastic iron uptake by rhizobia-infected cells in zone II.

Funding Projects

TypeCodeAcronymLeaderTitle
FP7335284METALSYMUNIVERSIDAD POLITECNICA DE MADRIDMetal transport in the tripartite symbiosis arbuscular mycorrhizal fungi-legume-rhizobia
FP7276771MENOMEDUNIVERSIDAD POLITECNICA DE MADRIDMetal homeostasis in nodulated Medicago plants
Government of SpainAGL-2012-32974UnspecifiedUnspecifiedUnspecified

More information

Item ID: 40465
DC Identifier: http://oa.upm.es/40465/
OAI Identifier: oai:oa.upm.es:40465
DOI: 10.1104/pp.114.254672
Official URL: http://www.plantphysiol.org/content/168/1/258.abstract
Deposited by: Memoria Investigacion
Deposited on: 17 May 2016 16:01
Last Modified: 05 Jun 2019 13:31
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