Identification of novel drought-tolerant-associated SNPs in common bean (Phaseolus vulgaris)

Villordo Pineda, Emiliano and González Chavira, Mario M. and Giraldo Carbajo, Patricia and Acosta Gallegos, Jorge A. and Caballero Pérez, Juan (2015). Identification of novel drought-tolerant-associated SNPs in common bean (Phaseolus vulgaris). "Frontiers in Plant Science", v. 6 ; pp. 1-9. ISSN 1664-462X. https://doi.org/10.3389/fpls.2015.00546.

Description

Title: Identification of novel drought-tolerant-associated SNPs in common bean (Phaseolus vulgaris)
Author/s:
  • Villordo Pineda, Emiliano
  • González Chavira, Mario M.
  • Giraldo Carbajo, Patricia
  • Acosta Gallegos, Jorge A.
  • Caballero Pérez, Juan
Item Type: Article
Título de Revista/Publicación: Frontiers in Plant Science
Date: 2015
ISSN: 1664-462X
Volume: 6
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Common bean (Phaseolus vulgaris L.) is a leguminous in high demand for human nutrition and a very important agricultural product. Production of common bean is constrained by environmental stresses such as drought. Although conventional plant selection has been used to increase production yield and stress tolerance, drought tolerance selection based on phenotype is complicated by associated physiological, anatomical, cellular, biochemical, and molecular changes. These changes are modulated by differential gene expression. A common method to identify genes associated with phenotypes of interest is the characterization of Single Nucleotide Polymorphims (SNPs) to link them to specific functions. In this work, we selected two drought-tolerant parental lines from Mesoamerica, Pinto Villa, and Pinto Saltillo. The parental lines were used to generate a population of 282 families (F3:5) and characterized by 169 SNPs. We associated the segregation of the molecular markers in our population with phenotypes including flowering time, physiological maturity, reproductive period, plant, seed and total biomass, reuse index, seed yield, weight of 100 seeds, and harvest index in three cultivation cycles. We observed 83 SNPs with significant association (p < 0.0003 after Bonferroni correction) with our quantified phenotypes. Phenotypes most associated were days to flowering and seed biomass with 58 and 44 associated SNPs, respectively. Thirty-seven out of the 83 SNPs were annotated to a gene with a potential function related to drought tolerance or relevant molecular/biochemical functions. Some SNPs such as SNP28 and SNP128 are related to starch biosynthesis, a common osmotic protector; and SNP18 is related to proline biosynthesis, another well-known osmotic protector.

More information

Item ID: 40809
DC Identifier: http://oa.upm.es/40809/
OAI Identifier: oai:oa.upm.es:40809
DOI: 10.3389/fpls.2015.00546
Official URL: http://journal.frontiersin.org/article/10.3389/fpls.2015.00546/full
Deposited by: Memoria Investigacion
Deposited on: 01 Jul 2016 17:32
Last Modified: 01 Jul 2016 17:32
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