Efficacy of supraspinatus tendon repair using mesenchymal stem cells along with a collagen I scaffold

Tornero-Esteban, Pilar; Hoyas, José Antonio; Villafuertes, Esther; Rodríguez-Bobada, Cruz; López-Gordillo, Yamila; Rojo Pérez, Francisco Javier; Guinea Tortuero, Gustavo V.; Paleczny, Anna; Lópiz-Morales, Yaiza; Rodríguez-Rodríguez, Luis; Marco, Fernando y Fernández-Gutiérrez, Benjamín (2015). Efficacy of supraspinatus tendon repair using mesenchymal stem cells along with a collagen I scaffold. "Journal of Orthopaedic Surgery and Research", v. 10 (n. 124); pp. 1-7. ISSN 1749-799X. https://doi.org/10.1186/s13018-015-0269-6.

Descripción

Título: Efficacy of supraspinatus tendon repair using mesenchymal stem cells along with a collagen I scaffold
Autor/es:
  • Tornero-Esteban, Pilar
  • Hoyas, José Antonio
  • Villafuertes, Esther
  • Rodríguez-Bobada, Cruz
  • López-Gordillo, Yamila
  • Rojo Pérez, Francisco Javier
  • Guinea Tortuero, Gustavo V.
  • Paleczny, Anna
  • Lópiz-Morales, Yaiza
  • Rodríguez-Rodríguez, Luis
  • Marco, Fernando
  • Fernández-Gutiérrez, Benjamín
Tipo de Documento: Artículo
Título de Revista/Publicación: Journal of Orthopaedic Surgery and Research
Fecha: 14 Agosto 2015
Volumen: 10
Materias:
Palabras Clave Informales: Mesenchymal stem cells (MSCs), animal model, rotator cuff
Escuela: E.T.S.I. Caminos, Canales y Puertos (UPM)
Departamento: Ciencia de los Materiales
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Objectives: Our main objective was to biologically improve rotator cuff healing in an elderly rat model using mesenchymal stem cells (MSCs) in combination with a collagen membrane and compared against other current techniques. Methods: A chronic rotator cuff tear injury model was developed by unilaterally detaching the supraspinatus (SP) tendons of Sprague-Dawley rats. At 1 month postinjury, the tears were repaired using one of the following techniques: (a) classical surgery using sutures (n = 12), (b) type I collagen membranes (n = 15), and (c) type I collagen membranes + 1 × 106 allogeneic MSCs (n = 14). Lesion restoration was evaluated at 1, 2, and 3 months postinjury based on biomechanical criteria. Continuous variables were described using mean and standard deviation (SD). To analyse the effect of the different surgical treatments in the repaired tendons’ biomechanical capabilities (máximum load, stiffness, and deformity), a two-way ANOVA model was used, introducing an interaction between such factor and time (1, 2, and 3 months postinjury). Results: With regard to maximum load, we observed an almost significant interaction between treatment and time (F = 2.62, df = 4, p = 0.053). When we analysed how this biomechanical capability changed with time for each treatment, we observed that repair with OrthADAPT and MSCs was associated with a significant increase in maximum load (p = 0.04) between months 1 and 3. On the other hand, when we compared the different treatments among themselves at different time points, we observed that the repair with OrthADAPT and MSCs has associated with a significant higher maximum load, when compared with the use of suture, but only at 3 months (p = 0.014). With regard to stiffness and deformity, no significant interaction was observed (F = 1.68, df = 4, p = 0.18; F = 0.40, df = 4, p = 0.81; respectively). Conclusions: The implantation of MSCs along with a collagen I scaffold into surgically created tendon defects is safe and effective. MSCs improved the tendon’s maximum load over time, indicating that MSCs could help facilitate the dynamic process of tendon repair.

Proyectos asociados

TipoCódigoAcrónimoResponsableTítulo
Gobierno de EspañaMAT 2009–10258Sin especificarSin especificarSin especificar

Más información

ID de Registro: 41128
Identificador DC: http://oa.upm.es/41128/
Identificador OAI: oai:oa.upm.es:41128
Identificador DOI: 10.1186/s13018-015-0269-6
URL Oficial: https://josr-online.biomedcentral.com/articles/10.1186/s13018-015-0269-6
Depositado por: Memoria Investigacion
Depositado el: 04 Jul 2016 16:20
Ultima Modificación: 04 Jul 2016 16:20
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