Efficacy of supraspinatus tendon repair using mesenchymal stem cells along with a collagen I scaffold

Tornero-Esteban, Pilar and Hoyas, José Antonio and Villafuertes, Esther and Rodríguez-Bobada, Cruz and López-Gordillo, Yamila and Rojo Pérez, Francisco Javier and Guinea Tortuero, Gustavo V. and Paleczny, Anna and Lópiz-Morales, Yaiza and Rodríguez-Rodríguez, Luis and Marco, Fernando and Fernández-Gutiérrez, Benjamín (2015). Efficacy of supraspinatus tendon repair using mesenchymal stem cells along with a collagen I scaffold. "Journal of Orthopaedic Surgery and Research", v. 10 (n. 124); pp. 1-7. ISSN 1749-799X. https://doi.org/10.1186/s13018-015-0269-6.

Description

Title: Efficacy of supraspinatus tendon repair using mesenchymal stem cells along with a collagen I scaffold
Author/s:
  • Tornero-Esteban, Pilar
  • Hoyas, José Antonio
  • Villafuertes, Esther
  • Rodríguez-Bobada, Cruz
  • López-Gordillo, Yamila
  • Rojo Pérez, Francisco Javier
  • Guinea Tortuero, Gustavo V.
  • Paleczny, Anna
  • Lópiz-Morales, Yaiza
  • Rodríguez-Rodríguez, Luis
  • Marco, Fernando
  • Fernández-Gutiérrez, Benjamín
Item Type: Article
Título de Revista/Publicación: Journal of Orthopaedic Surgery and Research
Date: 14 August 2015
ISSN: 1749-799X
Volume: 10
Subjects:
Freetext Keywords: Mesenchymal stem cells (MSCs), animal model, rotator cuff
Faculty: E.T.S.I. Caminos, Canales y Puertos (UPM)
Department: Ciencia de los Materiales
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Objectives: Our main objective was to biologically improve rotator cuff healing in an elderly rat model using mesenchymal stem cells (MSCs) in combination with a collagen membrane and compared against other current techniques. Methods: A chronic rotator cuff tear injury model was developed by unilaterally detaching the supraspinatus (SP) tendons of Sprague-Dawley rats. At 1 month postinjury, the tears were repaired using one of the following techniques: (a) classical surgery using sutures (n = 12), (b) type I collagen membranes (n = 15), and (c) type I collagen membranes + 1 × 106 allogeneic MSCs (n = 14). Lesion restoration was evaluated at 1, 2, and 3 months postinjury based on biomechanical criteria. Continuous variables were described using mean and standard deviation (SD). To analyse the effect of the different surgical treatments in the repaired tendons’ biomechanical capabilities (máximum load, stiffness, and deformity), a two-way ANOVA model was used, introducing an interaction between such factor and time (1, 2, and 3 months postinjury). Results: With regard to maximum load, we observed an almost significant interaction between treatment and time (F = 2.62, df = 4, p = 0.053). When we analysed how this biomechanical capability changed with time for each treatment, we observed that repair with OrthADAPT and MSCs was associated with a significant increase in maximum load (p = 0.04) between months 1 and 3. On the other hand, when we compared the different treatments among themselves at different time points, we observed that the repair with OrthADAPT and MSCs has associated with a significant higher maximum load, when compared with the use of suture, but only at 3 months (p = 0.014). With regard to stiffness and deformity, no significant interaction was observed (F = 1.68, df = 4, p = 0.18; F = 0.40, df = 4, p = 0.81; respectively). Conclusions: The implantation of MSCs along with a collagen I scaffold into surgically created tendon defects is safe and effective. MSCs improved the tendon’s maximum load over time, indicating that MSCs could help facilitate the dynamic process of tendon repair.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainMAT 2009–10258UnspecifiedUnspecifiedUnspecified
Madrid Regional GovernmentS2011/BMD-2460UnspecifiedUnspecifiedUnspecified
Government of SpainEC10-061UnspecifiedUnspecifiedUnspecified

More information

Item ID: 41128
DC Identifier: http://oa.upm.es/41128/
OAI Identifier: oai:oa.upm.es:41128
DOI: 10.1186/s13018-015-0269-6
Official URL: https://josr-online.biomedcentral.com/articles/10.1186/s13018-015-0269-6
Deposited by: Memoria Investigacion
Deposited on: 04 Jul 2016 16:20
Last Modified: 06 Jun 2019 15:08
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