Topographical and mechanical characterization of living eukaryotic cells on opaque substrates: development of a general procedure and its application to the study of non-adherent lymphocytes

Daza García, Rafael; Cruces, Julia; Arroyo-Hernández, María; Marí-Buyé, Núria; Fernández de la Fuente, Mónica; Plaza Baonza, Gustavo Ramón; Elices Calafat, Manuel; Pérez Rigueiro, José y Guinea Tortuero, Gustavo V. (2015). Topographical and mechanical characterization of living eukaryotic cells on opaque substrates: development of a general procedure and its application to the study of non-adherent lymphocytes. "Physical Biology", v. 12 (n. 2); pp. 1-12. ISSN 1478-3967. https://doi.org/10.1088/1478-3975/12/2/026005.

Descripción

Título: Topographical and mechanical characterization of living eukaryotic cells on opaque substrates: development of a general procedure and its application to the study of non-adherent lymphocytes
Autor/es:
  • Daza García, Rafael
  • Cruces, Julia
  • Arroyo-Hernández, María
  • Marí-Buyé, Núria
  • Fernández de la Fuente, Mónica
  • Plaza Baonza, Gustavo Ramón
  • Elices Calafat, Manuel
  • Pérez Rigueiro, José
  • Guinea Tortuero, Gustavo V.
Tipo de Documento: Artículo
Título de Revista/Publicación: Physical Biology
Fecha: 19 Marzo 2015
Volumen: 12
Materias:
Palabras Clave Informales: Atomic force microscopy; cell; mechanics
Escuela: E.T.S.I. Caminos, Canales y Puertos (UPM)
Departamento: Ciencia de los Materiales
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

The mechanical behavior of living murine T-lymphocytes was assessed by atomic force microscopy (AFM). A robust experimental procedure was developed to overcome some features of lymphocytes, in particular their spherical shape and non-adherent character. The procedure included the immobilization of the lymphocytes on amine-functionalized substrates, the use of hydrodynamic effects on the deflection of the AFM cantilever to monitor the approaching, and the use of the jumping mode for obtaining the images. Indentation curves were analyzed according to Hertz's model for contact mechanics. The calculated values of the elastic modulus are consistent both when considering the results obtained from a single lymphocyte and when comparing the curves recorded from cells of different specimens

Proyectos asociados

TipoCódigoAcrónimoResponsableTítulo
Gobierno de EspañaBFU2011-30336MAT 2009-10258Sin especificarSin especificar
Comunidad de MadridS2011BMD-2460Sin especificarSin especificar

Más información

ID de Registro: 41137
Identificador DC: http://oa.upm.es/41137/
Identificador OAI: oai:oa.upm.es:41137
Identificador DOI: 10.1088/1478-3975/12/2/026005
URL Oficial: http://iopscience.iop.org/article/10.1088/1478-3975/12/2/026005/meta;jsessionid=1E5D56ABD1DF6B44BEFC8F1EBDFD9934.c3.iopscience.cld.iop.org
Depositado por: Memoria Investigacion
Depositado el: 29 Jun 2016 14:14
Ultima Modificación: 29 Jun 2016 14:14
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