Topographical and mechanical characterization of living eukaryotic cells on opaque substrates: development of a general procedure and its application to the study of non-adherent lymphocytes

Daza García, Rafael and Cruces, Julia and Arroyo-Hernández, María and Marí-Buyé, Núria and Fernández de la Fuente, Mónica and Plaza Baonza, Gustavo Ramón and Elices Calafat, Manuel and Pérez Rigueiro, José and Guinea Tortuero, Gustavo V. (2015). Topographical and mechanical characterization of living eukaryotic cells on opaque substrates: development of a general procedure and its application to the study of non-adherent lymphocytes. "Physical Biology", v. 12 (n. 2); pp. 1-12. ISSN 1478-3967. https://doi.org/10.1088/1478-3975/12/2/026005.

Description

Title: Topographical and mechanical characterization of living eukaryotic cells on opaque substrates: development of a general procedure and its application to the study of non-adherent lymphocytes
Author/s:
  • Daza García, Rafael
  • Cruces, Julia
  • Arroyo-Hernández, María
  • Marí-Buyé, Núria
  • Fernández de la Fuente, Mónica
  • Plaza Baonza, Gustavo Ramón
  • Elices Calafat, Manuel
  • Pérez Rigueiro, José
  • Guinea Tortuero, Gustavo V.
Item Type: Article
Título de Revista/Publicación: Physical Biology
Date: 19 March 2015
ISSN: 1478-3967
Volume: 12
Subjects:
Freetext Keywords: Atomic force microscopy; cell; mechanics
Faculty: E.T.S.I. Caminos, Canales y Puertos (UPM)
Department: Ciencia de los Materiales
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The mechanical behavior of living murine T-lymphocytes was assessed by atomic force microscopy (AFM). A robust experimental procedure was developed to overcome some features of lymphocytes, in particular their spherical shape and non-adherent character. The procedure included the immobilization of the lymphocytes on amine-functionalized substrates, the use of hydrodynamic effects on the deflection of the AFM cantilever to monitor the approaching, and the use of the jumping mode for obtaining the images. Indentation curves were analyzed according to Hertz's model for contact mechanics. The calculated values of the elastic modulus are consistent both when considering the results obtained from a single lymphocyte and when comparing the curves recorded from cells of different specimens

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBFU2011-30336UnspecifiedUnspecifiedUnspecified
Madrid Regional GovernmentS2011/BMD-2460UnspecifiedUnspecifiedUnspecified
Government of SpainMAT2009-10258UnspecifiedUnspecifiedUnspecified

More information

Item ID: 41137
DC Identifier: http://oa.upm.es/41137/
OAI Identifier: oai:oa.upm.es:41137
DOI: 10.1088/1478-3975/12/2/026005
Official URL: http://iopscience.iop.org/article/10.1088/1478-3975/12/2/026005/meta;jsessionid=1E5D56ABD1DF6B44BEFC8F1EBDFD9934.c3.iopscience.cld.iop.org
Deposited by: Memoria Investigacion
Deposited on: 29 Jun 2016 14:14
Last Modified: 06 Jun 2019 15:18
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