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Daza García, Rafael and Cruces, Julia and Arroyo-Hernández, María and Marí-Buyé, Núria and Fernández de la Fuente, Mónica and Plaza Baonza, Gustavo Ramón and Elices Calafat, Manuel and Pérez Rigueiro, José and Guinea Tortuero, Gustavo V. (2015). Topographical and mechanical characterization of living eukaryotic cells on opaque substrates: development of a general procedure and its application to the study of non-adherent lymphocytes. "Physical Biology", v. 12 (n. 2); pp. 1-12. ISSN 1478-3967. https://doi.org/10.1088/1478-3975/12/2/026005.
Title: | Topographical and mechanical characterization of living eukaryotic cells on opaque substrates: development of a general procedure and its application to the study of non-adherent lymphocytes |
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Author/s: |
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Item Type: | Article |
Título de Revista/Publicación: | Physical Biology |
Date: | 19 March 2015 |
ISSN: | 1478-3967 |
Volume: | 12 |
Subjects: | |
Freetext Keywords: | Atomic force microscopy; cell; mechanics |
Faculty: | E.T.S.I. Caminos, Canales y Puertos (UPM) |
Department: | Ciencia de los Materiales |
Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
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The mechanical behavior of living murine T-lymphocytes was assessed by atomic force microscopy (AFM). A robust experimental procedure was developed to overcome some features of lymphocytes, in particular their spherical shape and non-adherent character. The procedure included the immobilization of the lymphocytes on amine-functionalized substrates, the use of hydrodynamic effects on the deflection of the AFM cantilever to monitor the approaching, and the use of the jumping mode for obtaining the images. Indentation curves were analyzed according to Hertz's model for contact mechanics. The calculated values of the elastic modulus are consistent both when considering the results obtained from a single lymphocyte and when comparing the curves recorded from cells of different specimens
Type | Code | Acronym | Leader | Title |
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Government of Spain | BFU2011-30336 | Unspecified | Unspecified | Unspecified |
Madrid Regional Government | S2011/BMD-2460 | Unspecified | Unspecified | Unspecified |
Government of Spain | MAT2009-10258 | Unspecified | Unspecified | Unspecified |
Item ID: | 41137 |
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DC Identifier: | http://oa.upm.es/41137/ |
OAI Identifier: | oai:oa.upm.es:41137 |
DOI: | 10.1088/1478-3975/12/2/026005 |
Official URL: | http://iopscience.iop.org/article/10.1088/1478-3975/12/2/026005/meta;jsessionid=1E5D56ABD1DF6B44BEFC8F1EBDFD9934.c3.iopscience.cld.iop.org |
Deposited by: | Memoria Investigacion |
Deposited on: | 29 Jun 2016 14:14 |
Last Modified: | 06 Jun 2019 15:18 |