Unexpected behavior of irradiated spider silk links conformational freedom to mechanical performance

Perea Abarca, Gracia Belén; Solanas, Concepción; Plaza Baonza, Gustavo Ramón; Guinea Tortuero, Gustavo V.; Jorge, Inmaculada; Vázquez Minguela, Jesús; Pérez Mateos, Jorge; Marí Buyé, Nuria; Elices Calafat, Manuel y Pérez Rigueiro, José (2015). Unexpected behavior of irradiated spider silk links conformational freedom to mechanical performance. "Soft Matter", v. 11 (n. 24); pp. 4868-4878. ISSN 1744-683X. https://doi.org/10.1039/c5sm00395d.

Descripción

Título: Unexpected behavior of irradiated spider silk links conformational freedom to mechanical performance
Autor/es:
  • Perea Abarca, Gracia Belén
  • Solanas, Concepción
  • Plaza Baonza, Gustavo Ramón
  • Guinea Tortuero, Gustavo V.
  • Jorge, Inmaculada
  • Vázquez Minguela, Jesús
  • Pérez Mateos, Jorge
  • Marí Buyé, Nuria
  • Elices Calafat, Manuel
  • Pérez Rigueiro, José
Tipo de Documento: Artículo
Título de Revista/Publicación: Soft Matter
Fecha: Mayo 2015
Volumen: 11
Materias:
Escuela: Centro de Tecnología Biomédica (CTB) (UPM)
Departamento: Ciencia de los Materiales
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Silk fibers from Argiope trifasciata and Nephila inaurata orb-web weaving spiders were UV irradiated to modify the molecular weight of the constituent proteins. Fibers were characterized either as forcibly silked or after being subjected to maximum supercontraction. The effect of irradiation on supercontraction was also studied, both in terms of the percentage of supercontraction and the tensile properties exhibited by irradiated and subsequently supercontracted fibers. The effects of UV exposure at the molecular level were assessed by polyacrylamide gel electrophoresis and mass spectrometry. It is shown that UV-irradiated fibers show a steady decrease in their main tensile parameters, most notably, tensile strength and strain. The combination of the mechanical and biochemical data suggests that the restricted conformational freedom of the proteins after UV irradiation is critical in the reduction of these properties. Consequently, an adequate topological organization of the protein chains emerges as a critical design principle in the performance of spider silk.

Proyectos asociados

TipoCódigoAcrónimoResponsableTítulo
Gobierno de EspañaMAT2012-38412-C02-01Sin especificarSin especificarSin especificar

Más información

ID de Registro: 41139
Identificador DC: http://oa.upm.es/41139/
Identificador OAI: oai:oa.upm.es:41139
Identificador DOI: 10.1039/c5sm00395d
URL Oficial: http://pubs.rsc.org/en/Content/ArticleLanding/2015/SM/C5SM00395D#!divAbstract
Depositado por: Memoria Investigacion
Depositado el: 25 Abr 2017 16:44
Ultima Modificación: 25 Abr 2017 16:44
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