Low-Intensity Pulsed Ultrasound Improves the Functional Properties of Cardiac Mesoangioblasts

Bernal, Aurora; Pérez, Laura M.; Lucas, Beatriz de; San Martín, Nuria; Kadow-Romacker, Anke; Plaza Baonza, Gustavo Ramón; Raum, Kay y Gálvez, Beatriz G. (2015). Low-Intensity Pulsed Ultrasound Improves the Functional Properties of Cardiac Mesoangioblasts. "Stem Cell Reviews and Reports", v. 11 (n. 6); pp. 852-865. ISSN 1550-8943. https://doi.org/10.1007/s12015-015-9608-6.

Descripción

Título: Low-Intensity Pulsed Ultrasound Improves the Functional Properties of Cardiac Mesoangioblasts
Autor/es:
  • Bernal, Aurora
  • Pérez, Laura M.
  • Lucas, Beatriz de
  • San Martín, Nuria
  • Kadow-Romacker, Anke
  • Plaza Baonza, Gustavo Ramón
  • Raum, Kay
  • Gálvez, Beatriz G.
Tipo de Documento: Artículo
Título de Revista/Publicación: Stem Cell Reviews and Reports
Fecha: Diciembre 2015
Volumen: 11
Materias:
Palabras Clave Informales: LIPUS; Cardiac mesoangioblasts; Migration; Differentiation; Cytoskeleton
Escuela: E.T.S.I. Caminos, Canales y Puertos (UPM)
Departamento: Ciencia de los Materiales
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Cell-based therapy is a promising approach for many diseases, including ischemic heart disease. Cardiac mesoangioblasts are committed vessel-associated progenitors that can restore to a significant, although partial, extent, heart structure and function in a murine model of myocardial infarction. Low-intensity pulsed ultrasound (LIPUS) is a noninvasive form of mechanical energy that can be delivered into biological tissues as acoustic pressure waves, and is widely used for clinical applications including bone fracture healing. We hypothesized that the positive effects of LIPUS on bone and soft tissue, such as increased cell differentiation and cytoskeleton reorganization, could be applied to increase the therapeutic potential of mesoangioblasts for heart repair. In this work, we show that LIPUS stimulation of cardiac mesoangioblasts isolated from mouse and human heart results in significant cellular modifications that provide beneficial effects to the cells, including increased malleability and improved motility. Additionally, LIPUS stimulation increased the number of binucleated cells and induced cardiac differentiation to an extent comparable with 5´-azacytidine treatment. Mechanistically, LIPUS stimulation activated the BMP-Smad signalling pathway and increased the expression of myosin light chain-2 together with upregulation of β1 integrin and RhoA, highlighting a potentially important role for cytoskeleton reorganization. Taken together, these results provide functional evidence that LIPUS might be a useful tool to explore in the field of heart cell therapy

Más información

ID de Registro: 41170
Identificador DC: http://oa.upm.es/41170/
Identificador OAI: oai:oa.upm.es:41170
Identificador DOI [BETA]: 10.1007/s12015-015-9608-6
URL Oficial: http://link.springer.com/article/10.1007%2Fs12015-015-9608-6
Depositado por: Memoria Investigacion
Depositado el: 28 Jun 2016 14:40
Ultima Modificación: 01 Ene 2017 23:30
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