Low-Intensity Pulsed Ultrasound Improves the Functional Properties of Cardiac Mesoangioblasts

Bernal, Aurora and Pérez, Laura M. and Lucas, Beatriz de and San Martín, Nuria and Kadow-Romacker, Anke and Plaza Baonza, Gustavo Ramón and Raum, Kay and Gálvez, Beatriz G. (2015). Low-Intensity Pulsed Ultrasound Improves the Functional Properties of Cardiac Mesoangioblasts. "Stem Cell Reviews and Reports", v. 11 (n. 6); pp. 852-865. ISSN 1550-8943. https://doi.org/10.1007/s12015-015-9608-6.

Description

Title: Low-Intensity Pulsed Ultrasound Improves the Functional Properties of Cardiac Mesoangioblasts
Author/s:
  • Bernal, Aurora
  • Pérez, Laura M.
  • Lucas, Beatriz de
  • San Martín, Nuria
  • Kadow-Romacker, Anke
  • Plaza Baonza, Gustavo Ramón
  • Raum, Kay
  • Gálvez, Beatriz G.
Item Type: Article
Título de Revista/Publicación: Stem Cell Reviews and Reports
Date: December 2015
ISSN: 1550-8943
Volume: 11
Subjects:
Freetext Keywords: LIPUS; Cardiac mesoangioblasts; Migration; Differentiation; Cytoskeleton
Faculty: E.T.S.I. Caminos, Canales y Puertos (UPM)
Department: Ciencia de los Materiales
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Cell-based therapy is a promising approach for many diseases, including ischemic heart disease. Cardiac mesoangioblasts are committed vessel-associated progenitors that can restore to a significant, although partial, extent, heart structure and function in a murine model of myocardial infarction. Low-intensity pulsed ultrasound (LIPUS) is a noninvasive form of mechanical energy that can be delivered into biological tissues as acoustic pressure waves, and is widely used for clinical applications including bone fracture healing. We hypothesized that the positive effects of LIPUS on bone and soft tissue, such as increased cell differentiation and cytoskeleton reorganization, could be applied to increase the therapeutic potential of mesoangioblasts for heart repair. In this work, we show that LIPUS stimulation of cardiac mesoangioblasts isolated from mouse and human heart results in significant cellular modifications that provide beneficial effects to the cells, including increased malleability and improved motility. Additionally, LIPUS stimulation increased the number of binucleated cells and induced cardiac differentiation to an extent comparable with 5´-azacytidine treatment. Mechanistically, LIPUS stimulation activated the BMP-Smad signalling pathway and increased the expression of myosin light chain-2 together with upregulation of β1 integrin and RhoA, highlighting a potentially important role for cytoskeleton reorganization. Taken together, these results provide functional evidence that LIPUS might be a useful tool to explore in the field of heart cell therapy

More information

Item ID: 41170
DC Identifier: http://oa.upm.es/41170/
OAI Identifier: oai:oa.upm.es:41170
DOI: 10.1007/s12015-015-9608-6
Official URL: http://link.springer.com/article/10.1007%2Fs12015-015-9608-6
Deposited by: Memoria Investigacion
Deposited on: 28 Jun 2016 14:40
Last Modified: 01 Jan 2017 23:30
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