Is Microarray Analysis Really Useful and Sufficient to Diagnose Nut Allergy in the Mediterranean Area?

Goikoetxea, M.J. and D'Amelio, C. M. and Martínez Aranguren, R. and Gamboa, P.M. and García, B. E. and Gómez, F. and Bartra, Joan and Alvarado, M. I. and Alonso, M. I. and González, E. and Terrados, S. and Moya Pérez, Carlos and Blanca-López, N. and Feo-Brito, F. and Villalba, M. and Diaz Perales, Araceli and Sanz, M.L. (2015). Is Microarray Analysis Really Useful and Sufficient to Diagnose Nut Allergy in the Mediterranean Area?. "Journal of Investigational Allergology And Clinical Immunology", v. 26 (n. 1); pp. 31-39. ISSN 1018-9068. https://doi.org/10.18176/jiaci.0005.

Description

Title: Is Microarray Analysis Really Useful and Sufficient to Diagnose Nut Allergy in the Mediterranean Area?
Author/s:
  • Goikoetxea, M.J.
  • D'Amelio, C. M.
  • Martínez Aranguren, R.
  • Gamboa, P.M.
  • García, B. E.
  • Gómez, F.
  • Bartra, Joan
  • Alvarado, M. I.
  • Alonso, M. I.
  • González, E.
  • Terrados, S.
  • Moya Pérez, Carlos
  • Blanca-López, N.
  • Feo-Brito, F.
  • Villalba, M.
  • Diaz Perales, Araceli
  • Sanz, M.L.
Item Type: Article
Título de Revista/Publicación: Journal of Investigational Allergology And Clinical Immunology
Date: 2015
Volume: 26
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Background: Component-based diagnosis on multiplex platforms is widely used in food allergy but its clinical performance has not been evaluated in nut allergy. Objective: To assess the diagnostic performance of a commercial protein microarray in the determination of specific IgE (sIgE) in peanut, hazelnut, and walnut allergy. Methods: sIgE was measured in 36 peanut-allergic, 36 hazelnut-allergic, and 44 walnut-allergic patients by ISAC 112, and subsequently, sIgE against available components was determined by ImmunoCAP in patients with negative ISAC results. ImmunoCAP was also used to measure sIgE to Ara h 9, Cor a 8, and Jug r 3 in a subgroup of lipid transfer protein (LTP)-sensitized nut-allergic patients (positive skin prick test to LTP-enriched extract). sIgE levels by ImmunoCAP were compared with ISAC ranges. Results: Most peanut-, hazelnut-, and walnut-allergic patients were sensitized to the corresponding nut LTP (Ara h 9, 66.7%; Cor a 8, 80.5%; Jug r 3, 84% respectively). However, ISAC did not detect sIgE in 33.3% of peanut-allergic patients, 13.9% of hazelnut-allergic patients, or 13.6% of walnut-allergic patients. sIgE determination by ImmunoCAP detected sensitization to Ara h 9, Cor a 8, and Jug r 3 in, respectively, 61.5% of peanut-allergic patients, 60% of hazelnut-allergic patients, and 88.3% of walnut-allergic patients with negative ISAC results. In the subgroup of peach LTP?sensitized patients, Ara h 9 sIgE was detected in more cases by ImmunoCAP than by ISAC (94.4% vs 72.2%, P<.05). Similar rates of Cor a 8 and Jug r 3 sensitization were detected by both techniques. Conclusions: The diagnostic performance of ISAC was adequate for hazelnut and walnut allergy but not for peanut allergy. sIgE sensitivity against Ara h 9 in ISAC needs to be improved.

More information

Item ID: 41251
DC Identifier: http://oa.upm.es/41251/
OAI Identifier: oai:oa.upm.es:41251
DOI: 10.18176/jiaci.0005
Official URL: http://www.jiaci.org/summary/vol26-issue1-num1291
Deposited by: Memoria Investigacion
Deposited on: 20 Jun 2016 16:54
Last Modified: 20 Jun 2016 16:54
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