Is Microarray Analysis Really Useful and Sufficient to Diagnose Nut Allergy in the Mediterranean Area?

Goikoetxea, M.J.; D'Amelio, C. M.; Martínez Aranguren, R.; Gamboa, P.M.; García, B. E.; Gómez, F.; Bartra, Joan; Alvarado, M. I.; Alonso, M. I.; González, E.; Terrados, S.; Moya Pérez, Carlos; Blanca-López, N.; Feo-Brito, F.; Villalba, M.; Diaz Perales, Araceli y Sanz, M.L. (2015). Is Microarray Analysis Really Useful and Sufficient to Diagnose Nut Allergy in the Mediterranean Area?. "Journal of Investigational Allergology And Clinical Immunology", v. 26 (n. 1); pp. 31-39. ISSN 1018-9068. https://doi.org/10.18176/jiaci.0005.

Descripción

Título: Is Microarray Analysis Really Useful and Sufficient to Diagnose Nut Allergy in the Mediterranean Area?
Autor/es:
  • Goikoetxea, M.J.
  • D'Amelio, C. M.
  • Martínez Aranguren, R.
  • Gamboa, P.M.
  • García, B. E.
  • Gómez, F.
  • Bartra, Joan
  • Alvarado, M. I.
  • Alonso, M. I.
  • González, E.
  • Terrados, S.
  • Moya Pérez, Carlos
  • Blanca-López, N.
  • Feo-Brito, F.
  • Villalba, M.
  • Diaz Perales, Araceli
  • Sanz, M.L.
Tipo de Documento: Artículo
Título de Revista/Publicación: Journal of Investigational Allergology And Clinical Immunology
Fecha: 2015
Volumen: 26
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnología - Biología Vegetal
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

Texto completo

[img]
Vista Previa
PDF (Document Portable Format) - Se necesita un visor de ficheros PDF, como GSview, Xpdf o Adobe Acrobat Reader
Descargar (1MB) | Vista Previa

Resumen

Background: Component-based diagnosis on multiplex platforms is widely used in food allergy but its clinical performance has not been evaluated in nut allergy. Objective: To assess the diagnostic performance of a commercial protein microarray in the determination of specific IgE (sIgE) in peanut, hazelnut, and walnut allergy. Methods: sIgE was measured in 36 peanut-allergic, 36 hazelnut-allergic, and 44 walnut-allergic patients by ISAC 112, and subsequently, sIgE against available components was determined by ImmunoCAP in patients with negative ISAC results. ImmunoCAP was also used to measure sIgE to Ara h 9, Cor a 8, and Jug r 3 in a subgroup of lipid transfer protein (LTP)-sensitized nut-allergic patients (positive skin prick test to LTP-enriched extract). sIgE levels by ImmunoCAP were compared with ISAC ranges. Results: Most peanut-, hazelnut-, and walnut-allergic patients were sensitized to the corresponding nut LTP (Ara h 9, 66.7%; Cor a 8, 80.5%; Jug r 3, 84% respectively). However, ISAC did not detect sIgE in 33.3% of peanut-allergic patients, 13.9% of hazelnut-allergic patients, or 13.6% of walnut-allergic patients. sIgE determination by ImmunoCAP detected sensitization to Ara h 9, Cor a 8, and Jug r 3 in, respectively, 61.5% of peanut-allergic patients, 60% of hazelnut-allergic patients, and 88.3% of walnut-allergic patients with negative ISAC results. In the subgroup of peach LTP?sensitized patients, Ara h 9 sIgE was detected in more cases by ImmunoCAP than by ISAC (94.4% vs 72.2%, P<.05). Similar rates of Cor a 8 and Jug r 3 sensitization were detected by both techniques. Conclusions: The diagnostic performance of ISAC was adequate for hazelnut and walnut allergy but not for peanut allergy. sIgE sensitivity against Ara h 9 in ISAC needs to be improved.

Más información

ID de Registro: 41251
Identificador DC: http://oa.upm.es/41251/
Identificador OAI: oai:oa.upm.es:41251
Identificador DOI: 10.18176/jiaci.0005
URL Oficial: http://www.jiaci.org/summary/vol26-issue1-num1291
Depositado por: Memoria Investigacion
Depositado el: 20 Jun 2016 16:54
Ultima Modificación: 20 Jun 2016 16:54
  • Open Access
  • Open Access
  • Sherpa-Romeo
    Compruebe si la revista anglosajona en la que ha publicado un artículo permite también su publicación en abierto.
  • Dulcinea
    Compruebe si la revista española en la que ha publicado un artículo permite también su publicación en abierto.
  • Recolecta
  • e-ciencia
  • Observatorio I+D+i UPM
  • OpenCourseWare UPM