A Recombinant Sal k 1 Isoform as an Alternative to the Polymorphic Allergen from Salsola kali Pollen for Allergy Diagnosis

Mas, Salvador; Boissy, Patrice; Monsalve, Rafael I.; Cuesta Herranz, J.; Diaz Perales, Araceli; Fernández, Javier; Colás, Carlos; Rodríguez, Rosalía; Barderas, Rodrigo y Villalba, M. (2015). A Recombinant Sal k 1 Isoform as an Alternative to the Polymorphic Allergen from Salsola kali Pollen for Allergy Diagnosis. "International Archives of Allergy And Immunology", v. 167 (n. 2); pp. 83-93. ISSN 1018-2438. https://doi.org/10.1159/000434680.

Descripción

Título: A Recombinant Sal k 1 Isoform as an Alternative to the Polymorphic Allergen from Salsola kali Pollen for Allergy Diagnosis
Autor/es:
  • Mas, Salvador
  • Boissy, Patrice
  • Monsalve, Rafael I.
  • Cuesta Herranz, J.
  • Diaz Perales, Araceli
  • Fernández, Javier
  • Colás, Carlos
  • Rodríguez, Rosalía
  • Barderas, Rodrigo
  • Villalba, M.
Tipo de Documento: Artículo
Título de Revista/Publicación: International Archives of Allergy And Immunology
Fecha: Septiembre 2015
Volumen: 167
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnología - Biología Vegetal
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

The incidence of Amaranthaceae pollen allergy has increased due to the desertification occurring in many countries. In some regions of Spain, Salsola kali is the main cause of pollinosis, at almost the same level as olive and grass pollen. Sal k 1 - the sensitization marker of S. kali pollinosis - is used in clinical diagnosis, but is purified at a low yield from pollen. We aimed to produce a recombinant (r)Sal k 1 able to span the structural and immunological properties of the natural isoforms from pollen, and validate its potential use for diagnosis. METHODS: Specific cDNA was amplified by PCR, cloned into the pET41b vector and used to transform BL21 (DE3) Escherichia coli cells. Immunoblotting, ELISA, basophil activation and skin-prick tests were used to validate the recombinant protein against Sal k 1 isolated from pollen. Sera and blood cells from S. kali pollen-sensitized patients and specific monoclonal and polyclonal antisera were used. RESULTS: rSal k 1 was produced in bacteria with a yield of 7.5 mg/l of cell culture. The protein was purified to homogeneity and structural and immunologically validated against the natural form. rSal k 1 exhibited a higher IgE cross-reactivity with plant-derived food extracts such as peanut, almond or tomato than with pollen sources such as Platanus acerifolia and Oleaceae members. CONCLUSIONS: rSal k 1 expressed in bacteria retains intact structural and immunological properties in comparison to the pollen-derived allergen. It spans the immunological properties of most of the isoforms found in pollen, and it might substitute natural Sal k 1 in clinical diagnosis.

Más información

ID de Registro: 41287
Identificador DC: http://oa.upm.es/41287/
Identificador OAI: oai:oa.upm.es:41287
Identificador DOI: 10.1159/000434680
URL Oficial: http://content.karger.com/Article/Abstract/434680
Depositado por: Memoria Investigacion
Depositado el: 21 Jun 2016 15:30
Ultima Modificación: 21 Jun 2016 15:30
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