D-Root: a system for cultivating plants with the roots in darkness or under different light conditions

Silva, Javier and Moreno Risueño, Miguel Ángel and Manzano, Concepción and Pallero Baena, Mercedes and Navarro Neila, Sara and Téllez Robledo, Bárbara and García Mina, Jose María and Baigorri, Roberto and Gallego Rodriguez, Francisco Javier and Pozo Benito, Juan Carlos del (2015). D-Root: a system for cultivating plants with the roots in darkness or under different light conditions. "Plant Journal", v. 84 (n. 1); pp. 244-255. ISSN 0960-7412. https://doi.org/10.1111/tpj.12998.

Description

Title: D-Root: a system for cultivating plants with the roots in darkness or under different light conditions
Author/s:
  • Silva, Javier
  • Moreno Risueño, Miguel Ángel
  • Manzano, Concepción
  • Pallero Baena, Mercedes
  • Navarro Neila, Sara
  • Téllez Robledo, Bárbara
  • García Mina, Jose María
  • Baigorri, Roberto
  • Gallego Rodriguez, Francisco Javier
  • Pozo Benito, Juan Carlos del
Item Type: Article
Título de Revista/Publicación: Plant Journal
Date: October 2015
ISSN: 0960-7412
Volume: 84
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

In nature roots grow in the dark and away from light (negative phototropism). However, most current research in root biology has been carried out with the root system grown in the presence of light. Here, we have engineered a device, called Dark-Root (D-Root), to grow plants in vitro with the aerial part exposed to the normal light/dark photoperiod while the roots are in the dark or exposed to specific wavelengths or light intensities. D-Root provides an efficient system for cultivating a large number of seedlings and easily characterizing root architecture in the dark. At the morphological level, root illumination shortens root length and promotes early emergence of lateral roots, therefore inducing expansion of the root system. Surprisingly, root illumination also affects shoot development, including flowering time. Our analyses also show that root illumination alters the proper response to hormones or abiotic stress (e.g. salt or osmotic stress) and nutrient starvation, enhancing inhibition of root growth. In conclusion, D-Root provides a growing system closer to the natural one for assaying Arabidopsis plants, and therefore its use will contribute to a better understanding of the mechanisms involved in root development, hormonal signaling and stress responses.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBFU2013-41160-PUnspecifiedUnspecifiedIdentificacion y caracterización de los determinantes moleculares de la pluripotencia adulta en plantas usando la raíz de arabidopsis
Government of SpainBIO2011‐28184‐C02‐01UnspecifiedUnspecifiedUnspecified
FP7322082NEW ROOT STEM CELLSUnspecifiedA molecular, genomics and genetic analysis of new stem cell formation during root morphogenesis

More information

Item ID: 41311
DC Identifier: http://oa.upm.es/41311/
OAI Identifier: oai:oa.upm.es:41311
DOI: 10.1111/tpj.12998
Official URL: http://onlinelibrary.wiley.com/doi/10.1111/tpj.12998/full
Deposited by: Memoria Investigacion
Deposited on: 27 Oct 2016 12:57
Last Modified: 07 Jun 2019 13:36
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