MtCOPT1 mediates copper transport to Medicago truncatula nodules

Senovilla Ramos, Marta and Castro Rodríguez, Rosario and Imperial Ródenas, Juan and Gonzalez Guerrero, Manuel (2015). MtCOPT1 mediates copper transport to Medicago truncatula nodules. In: "IV Portuguese-Spanish Congress on Nitrogen Fixation. XV Nationa Meeting of the Spanish Society of Nitrogen Fixation", 16/06/2015-18/06/2015, León. p. 1.

Description

Title: MtCOPT1 mediates copper transport to Medicago truncatula nodules
Author/s:
  • Senovilla Ramos, Marta
  • Castro Rodríguez, Rosario
  • Imperial Ródenas, Juan
  • Gonzalez Guerrero, Manuel
Item Type: Presentation at Congress or Conference (Article)
Event Title: IV Portuguese-Spanish Congress on Nitrogen Fixation. XV Nationa Meeting of the Spanish Society of Nitrogen Fixation
Event Dates: 16/06/2015-18/06/2015
Event Location: León
Title of Book: IV Portuguese-Spanish Congress on Nitrogen Fixation. XV Nationa Meeting of the Spanish Society of Nitrogen Fixation
Date: 2015
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Copper is an essential oligonutrient. Its redox properties allow it to be a suitable cofactor for many proteins, such as cytochromes or superoxide dismutases. Copper is key for Symbiotic Nitrogen Fixation (SNF); For instance, bacteroids contain copper-dependent cytochrome oxidases that provide energy in the microaerobic environment within the nodule. Once copper is in the plant, it is delivered by the vasculature to the apoplast of zone II. From there, a plasma membrane transporter introduces this nutrient into the cell for copper-protein assembly. COPT family transporters mediate high-affinity copper transport towards the cytosol. Therefore, they are good candidates to introduce copper in nodule cells. From the 8 COPT family genes present in M. truncatula genome, MtCOPT1 is the only one induced specifically in nodule. MtCOPT1 can restore Saccharomyces cerevisae ?ctr1 capacity to uptake copper. Inmunolocalization and GUS fusion studies localize MtCOPT1 in the nodule. Moreover, a Tnt-1-derived knockdown mutant line for MtCOPT1 shows decreased nitrogenase activity when compared with the wild-type line. This activity is, at least, partially rescued when a wild-type copy of MtCOPT1 gene is reintroduced. Taken together, all this data suggest an important role of MtCOPT1 copper-mediated transport for SNF.

Funding Projects

TypeCodeAcronymLeaderTitle
FP7IDEAS-ERC-335284METALSYMUNIVERSIDAD POLITECNICA DE MADRIDMetal transport in the tripartite symbiosis arbuscular mycorrhizal fungi-legume-rhizobia
Government of SpainAGL-2012-32974UnspecifiedUniversidad Politécnica de MadridTransporte de metales a los simbiosomas de medicago truncatula

More information

Item ID: 41840
DC Identifier: http://oa.upm.es/41840/
OAI Identifier: oai:oa.upm.es:41840
Deposited by: Memoria Investigacion
Deposited on: 12 Jan 2017 18:05
Last Modified: 12 Jan 2017 18:05
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