MtNramp1 is responsible for iron uptake by rhizobia infected cells in Medicago truncatula nodules

Tejada Jiménez, Manuel; Castro Rodríguez, Rosario; Kryvoruchko, Igor; Lucas, M. Mercedes; Udvardi, Michael; Imperial Ródenas, Juan y Gonzalez Guerrero, Manuel (2015). MtNramp1 is responsible for iron uptake by rhizobia infected cells in Medicago truncatula nodules. En: "XV Congress of the Spanish Society of Nitrogen Fixation, and the IV Portuguese-Spanish Congress on Nitrogen Fixation", 16/06/2015-18/06/2015, León, España. p. 1.

Descripción

Título: MtNramp1 is responsible for iron uptake by rhizobia infected cells in Medicago truncatula nodules
Autor/es:
  • Tejada Jiménez, Manuel
  • Castro Rodríguez, Rosario
  • Kryvoruchko, Igor
  • Lucas, M. Mercedes
  • Udvardi, Michael
  • Imperial Ródenas, Juan
  • Gonzalez Guerrero, Manuel
Tipo de Documento: Ponencia en Congreso o Jornada (Artículo)
Título del Evento: XV Congress of the Spanish Society of Nitrogen Fixation, and the IV Portuguese-Spanish Congress on Nitrogen Fixation
Fechas del Evento: 16/06/2015-18/06/2015
Lugar del Evento: León, España
Título del Libro: XV Congress of the Spanish Society of Nitrogen Fixation, and the IV Portuguese-Spanish Congress on Nitrogen Fixation
Fecha: 2015
Materias:
Escuela: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Departamento: Otro
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

Texto completo

[img]
Vista Previa
PDF (Document Portable Format) - Se necesita un visor de ficheros PDF, como GSview, Xpdf o Adobe Acrobat Reader
Descargar (98kB) | Vista Previa

Resumen

All known organisms need iron to accomplish important biological processes for life, ranging from gene transcription to respiration. Particularly in symbiotic nitrogen fixation (SNF) iron plays a critical role since the activity of key proteins involved in this process, such as nitrogenase, leghemoglobin, Fe-superoxide dismutase and other proteins involved in energy transduction, directly depends on the presence of iron as cofactor in their active centre. In the model legume Medicago truncatula, iron is delivered by the vasculature and released in the apoplast on the zone II of the nodule (infection/maturation zone). Then iron moves into rhizobia-infected cells and it is used in the synthesis of iron-containing proteins. Therefore, different iron transporters should mediate iron traffic through the plasma membrane of plant cells and the symbiosome membrane. However, no candidates were available to be responsible for iron transport across the plasma membrane from the nodule apoplast to rhizobia-infected cells. In the present work, we have identified a Nramp member gene from M. truncatula (MtNramp1) as responsible for iron transport from nodule apoplast into rhizobia-infected cell. MtNramp1 shows the highest expression in the nodule among the seven Nramp genes present in M. truncatula genome. Immunolocalization studies show that MtNramp1 is located in the plasma membrane of zone II nodule cells. A loss-of-function nramp1 mutant presented impaired growth specifically under symbiotic conditions, concomitant with a lower nitrogenase activity compared to wild-type plants. This phenotype was rescued by the addition of iron to the nutritive solution or by complementation of a mutant with a wild-type Nramp1 copy. Furthermore yeast complementation assays using mutant affected on iron transport pointed to a role of MrNramp1 in iron transport toward the cytosol. All together, these results point to a role of MtNramp1 in iron supply to nodule cells connected to SNF, and represent an important step toward the understanding of iron incorporation and homeostasis in plant nitrogen-fixing tissues.

Proyectos asociados

TipoCódigoAcrónimoResponsableTítulo
FP7IDEAS-ERC-335284METALSYMUNIVERSIDAD POLITECNICA DE MADRIDMetal transport in the tripartite symbiosis arbuscular mycorrhizal fungi-legume-rhizobia
Comunidad de MadridAGL-2012-32974Sin especificarUniversidad Politécnica de MadridTransporte de metales a los simbiosomas de medicago truncatula

Más información

ID de Registro: 41841
Identificador DC: http://oa.upm.es/41841/
Identificador OAI: oai:oa.upm.es:41841
URL Oficial: http://congreso.xvsefin.unileon.es/
Depositado por: Memoria Investigacion
Depositado el: 28 Oct 2016 13:19
Ultima Modificación: 28 Oct 2016 13:19
  • Open Access
  • Open Access
  • Sherpa-Romeo
    Compruebe si la revista anglosajona en la que ha publicado un artículo permite también su publicación en abierto.
  • Dulcinea
    Compruebe si la revista española en la que ha publicado un artículo permite también su publicación en abierto.
  • Recolecta
  • e-ciencia
  • Observatorio I+D+i UPM
  • OpenCourseWare UPM