Engineering nitrogen-fixing plants: multigene transfer, targeting of expression to organelles and tissues, and other challenges

Caro Bernat, Elena (2016). Engineering nitrogen-fixing plants: multigene transfer, targeting of expression to organelles and tissues, and other challenges. In: "2nd Synthetic Biology Congress", 20/10/2016-21/10-2016, Londres (Reino Unido). p. 1.

Description

Title: Engineering nitrogen-fixing plants: multigene transfer, targeting of expression to organelles and tissues, and other challenges
Author/s:
  • Caro Bernat, Elena
Item Type: Presentation at Congress or Conference (Article)
Event Title: 2nd Synthetic Biology Congress
Event Dates: 20/10/2016-21/10-2016
Event Location: Londres (Reino Unido)
Title of Book: Second Synthetic Biology Congress
Date: 2016
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Biological N2 fixation, catalyzed by the prokaryotic enzyme nitrogenase, is an alternative to the use of synthetic N fertilizers to increase cereal production yields. The strategy we are pursuing in an effort to increase cereal crops productivity is the direct transfer of nif prokaryotic genes into cereals. Because Nitrogenase is an O2-labile metalloenzyme which biosynthesis requires a complex pathway where numerous nif gene products are involved, the main challenges we face are the control of gene expression and protein accumulation in an O2 depleted environment and the stable and high expression of multiple transgenes. Our efforts are now focused on controlling expression targeting to different tissues and organelles and on studying the signals in transgenes that license gene silencing.

More information

Item ID: 41877
DC Identifier: http://oa.upm.es/41877/
OAI Identifier: oai:oa.upm.es:41877
Official URL: http://www.globalengage.co.uk/synthetic-biology.html
Deposited by: Memoria Investigacion
Deposited on: 15 Feb 2017 15:45
Last Modified: 15 Feb 2017 15:45
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