Genomic analysis of three Bradyrhizobium geno(species) nodulating Lima bean (Phaseolus lunatus L.) in Peru

Ormeño-Orrillo, E. and Zúñiga Dávila, D. and Rey Navarro, Luis and Duran Wendt, David Ricardo and Imperial Ródenas, Juan and Ruiz Argueso, Tomás and Martínez Romero, Esperanza (2015). Genomic analysis of three Bradyrhizobium geno(species) nodulating Lima bean (Phaseolus lunatus L.) in Peru. In: "XV Reunión Nacional de Fijación de Nitrógeno y IV Congreso Luso-Español de Fijación de Nitrógeno", 16/06/2015-18/06/2015, León. p. 1.

Description

Title: Genomic analysis of three Bradyrhizobium geno(species) nodulating Lima bean (Phaseolus lunatus L.) in Peru
Author/s:
  • Ormeño-Orrillo, E.
  • Zúñiga Dávila, D.
  • Rey Navarro, Luis
  • Duran Wendt, David Ricardo
  • Imperial Ródenas, Juan
  • Ruiz Argueso, Tomás
  • Martínez Romero, Esperanza
Item Type: Presentation at Congress or Conference (Article)
Event Title: XV Reunión Nacional de Fijación de Nitrógeno y IV Congreso Luso-Español de Fijación de Nitrógeno
Event Dates: 16/06/2015-18/06/2015
Event Location: León
Title of Book: XV SEFIN
Date: 2015
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The Lima bean (Phaseolus lunatus), also known as pallar, ibes, garrofón or butter bean in Peru, México, Spain and USA, respectively, is the second most economically important species of Phaseolus. Peru is a centre of origin and domestication of Lima bean. This crop is cultivated mainly in the Central coast of Peru under a subtropical arid climate. In contrast to the common bean (Phaseolus vulgaris) which forms nodules with fast growing Rhizobium strains, the Lima bean forms nodules with slow growing bacteria of the Bradyrhizobium genus (López-López et al. 2013, Ormeño-Orrillo et al. 2006). We found strains of Bradyrhizobium yuanmingense and of three novel Bradyrhizobium genospecies inside P. lunatus nodules in Peru (Ormeño- Orrillo et al. 2006). Strains of the three novel genospecies were characterized by showing an extra-slow growing phenotype (generation time > 10 h-1) and strong alkali production in yeast extract mannitol medium. Two of the novel genospecies were recently named as Bradyrhizobium paxllaeri and Bradyrhizobium icense (Durán et al. 2014). B. paxllaeri strains dominate nodule occupancy followed by those of B. icense and then the third and yet-unnamed genospecies. With the aim to gain insights into this differential competitive ability, we sequenced the genome of one representative strain of each species. Sequencing was performed with the Illumina HiSeq or MiSeq platform and genome assembly with the SPAdes program. Gene prediction and automated annotation was performed with Prokka and RAST. Annotation of genes putatively involved in competitiveness was manually curated. Assemblies had from 55 to 175 contigs, with N50 sizes > 131 kb. Genome sizes of B. paxllaeri and B. icense were similar (8.2 Mb) and larger than that of the third genospecies (7.8 Mb). Preliminary analysis revealed differences between B. paxllaeri and the other two genospecies such as more genes for type IV pilus and two nodA genes. A comparative genomic analysis of P. lunatus symbionts will be presented at the meeting.

More information

Item ID: 41882
DC Identifier: http://oa.upm.es/41882/
OAI Identifier: oai:oa.upm.es:41882
Official URL: http://www.ugr.es/~sefin/
Deposited by: Memoria Investigacion
Deposited on: 22 Sep 2016 14:48
Last Modified: 22 Sep 2016 14:48
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