Induction of ovulation in rabbit does using purified nerve growth factor and camel seminal plasma

Masdeu, M. and García García, R. M. and Cardinali, R. and Millán, P. and Arias Álvarez, M. and Castellini, C. and Lorenzo, P.L. and Garcia Rebollar, Pilar (2015). Induction of ovulation in rabbit does using purified nerve growth factor and camel seminal plasma. In: "41st Annual Conference of the IETS", 10/01/2015-13/01/2015, Versalles (Francia). p. 1.

Description

Title: Induction of ovulation in rabbit does using purified nerve growth factor and camel seminal plasma
Author/s:
  • Masdeu, M.
  • García García, R. M.
  • Cardinali, R.
  • Millán, P.
  • Arias Álvarez, M.
  • Castellini, C.
  • Lorenzo, P.L.
  • Garcia Rebollar, Pilar
Item Type: Presentation at Congress or Conference (Article)
Event Title: 41st Annual Conference of the IETS
Event Dates: 10/01/2015-13/01/2015
Event Location: Versalles (Francia)
Title of Book: 41st Annual Conference of the IETS
Date: 2015
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Producción Agraria
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The presence of an ovulation-inducing factor (OIF) in the seminal plasma (SP) of several species with spontaneous and induced ovulation, including the rabbit, has been documented. Recent studies have demonstrated that the OIF in the SP of camels (SPCAM) is a nerve growth factor (β-NGF). The aim of this study was to determine if purified β-NGF from mouse submandibular glands or SPCAM could provoke ovulation induction in the rabbit doe. A total of 35 females were synchronized with 25 IU of equine chorionic gonadotropin (Serigan, Laboratorios Ovejero, Spain) and allocated into 4 groups. Forty-eight hours later (Day 0), does were given a single dose (IM) of 1 mL of saline solution (SS; n = 8); 1 mL of gonadorelin (GnRH; Inducel, Laboratorios Ovejero, Spain; n = 9); 24 µg of β-NGF (2.5S-NGF; Promega, USA; n = 10); or 1 mL of centrifuged raw camel SP (SPCAM; 127 pg mL–1 NGF; n = 8). After treatment, an empty catheter was introduced through the vagina to simulate the nervous/mechanical stimulus of coitus (4 animals per group). Plasma LH concentrations were determined in blood samples taken 30 min before treatment and at 0, 30, 60, 90, and 120 min after injection. Progesterone concentrations were assessed at 0 and 120 min and every 2 days until Day 6 after treatment. Concentrations of β-NGF in camel SP and hormone determinations were made by enzyme immunoassay. Ovulation rate (OR) was determined after euthanasia on Day 7.

More information

Item ID: 41936
DC Identifier: http://oa.upm.es/41936/
OAI Identifier: oai:oa.upm.es:41936
Official URL: http://www.iets.org/2015/
Deposited by: Memoria Investigacion
Deposited on: 28 Jun 2016 13:39
Last Modified: 28 Jun 2016 13:39
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