Induction of ovulation in rabbit does using purified nerve growth factor and camel seminal plasma

Masdeu, M.; García García, R. M.; Cardinali, R.; Millán, P.; Arias Álvarez, M.; Castellini, C.; Lorenzo, P.L. y Garcia Rebollar, Pilar (2015). Induction of ovulation in rabbit does using purified nerve growth factor and camel seminal plasma. En: "41st Annual Conference of the IETS", 10/01/2015-13/01/2015, Versalles (Francia). p. 1.

Descripción

Título: Induction of ovulation in rabbit does using purified nerve growth factor and camel seminal plasma
Autor/es:
  • Masdeu, M.
  • García García, R. M.
  • Cardinali, R.
  • Millán, P.
  • Arias Álvarez, M.
  • Castellini, C.
  • Lorenzo, P.L.
  • Garcia Rebollar, Pilar
Tipo de Documento: Ponencia en Congreso o Jornada (Artículo)
Título del Evento: 41st Annual Conference of the IETS
Fechas del Evento: 10/01/2015-13/01/2015
Lugar del Evento: Versalles (Francia)
Título del Libro: 41st Annual Conference of the IETS
Fecha: 2015
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Producción Agraria
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

The presence of an ovulation-inducing factor (OIF) in the seminal plasma (SP) of several species with spontaneous and induced ovulation, including the rabbit, has been documented. Recent studies have demonstrated that the OIF in the SP of camels (SPCAM) is a nerve growth factor (β-NGF). The aim of this study was to determine if purified β-NGF from mouse submandibular glands or SPCAM could provoke ovulation induction in the rabbit doe. A total of 35 females were synchronized with 25 IU of equine chorionic gonadotropin (Serigan, Laboratorios Ovejero, Spain) and allocated into 4 groups. Forty-eight hours later (Day 0), does were given a single dose (IM) of 1 mL of saline solution (SS; n = 8); 1 mL of gonadorelin (GnRH; Inducel, Laboratorios Ovejero, Spain; n = 9); 24 µg of β-NGF (2.5S-NGF; Promega, USA; n = 10); or 1 mL of centrifuged raw camel SP (SPCAM; 127 pg mL–1 NGF; n = 8). After treatment, an empty catheter was introduced through the vagina to simulate the nervous/mechanical stimulus of coitus (4 animals per group). Plasma LH concentrations were determined in blood samples taken 30 min before treatment and at 0, 30, 60, 90, and 120 min after injection. Progesterone concentrations were assessed at 0 and 120 min and every 2 days until Day 6 after treatment. Concentrations of β-NGF in camel SP and hormone determinations were made by enzyme immunoassay. Ovulation rate (OR) was determined after euthanasia on Day 7.

Más información

ID de Registro: 41936
Identificador DC: http://oa.upm.es/41936/
Identificador OAI: oai:oa.upm.es:41936
URL Oficial: http://www.iets.org/2015/
Depositado por: Memoria Investigacion
Depositado el: 28 Jun 2016 13:39
Ultima Modificación: 28 Jun 2016 13:39
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