Novel winter-associated regulators of the circadian clock in poplar

Ramos Sanchez, Jose Manuel and Moreno Cortes, Alicia and Conde Rodriguez, Daniel and Hernandez Verdeja, Tamara and Perales, Mariano and Allona Alberich, Isabel Marta (2015). Novel winter-associated regulators of the circadian clock in poplar. In: "IUFRO Tree Biotechnology 2015 Conference", 08/06/2015-12/06/2016, Florencia, Italia. p. 1.

Description

Title: Novel winter-associated regulators of the circadian clock in poplar
Author/s:
  • Ramos Sanchez, Jose Manuel
  • Moreno Cortes, Alicia
  • Conde Rodriguez, Daniel
  • Hernandez Verdeja, Tamara
  • Perales, Mariano
  • Allona Alberich, Isabel Marta
Item Type: Presentation at Congress or Conference (Article)
Event Title: IUFRO Tree Biotechnology 2015 Conference
Event Dates: 08/06/2015-12/06/2016
Event Location: Florencia, Italia
Title of Book: Tree Biotechnology 2015 Conference: Forest: the importance to the planet and society
Date: 2015
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Background Winter dormancy is an adaptive mechanism that allows trees from temperate and cold regions to survive the harsh conditions of this season. Critical steps of this process are strongly influenced by environmental cues, mainly daylength and temperature. The mechanism that integrates these signals is the circadian clock. Despite the importance of the correct functioning of the clock for the healthy state of the plant [1], low temperatures cause the disruption of the circadian clock in trees, which consists in a transcriptional activation followed by an arrhythmic expression [2-5]. In this work we uncover winter-associated regulators of the circadian clock in poplar. Methods Firstly, we made a transcriptional fusion with the promoter of LHY2, a circadian clock gene, and the luciferase gene. This construct was used to generate transgenic poplars (717-1B4, INRA clone). With these events we characterized the expression of this promoter under different conditions of photoperiod and temperature. To this aim we have set up a circadian luminiscence assay registering luciferase activity from leaf discs with a luminometer. Then we carried out a Yeast One Hybrid (Y1H) screening with a library enriched in winter-associated factors and using this promoter as bait. Candidate regulators are tested in vivo using Golden Braid technology [6] and transient assays in poplar, by which we overexpressed and silenced the candidate genes. Results and Conclusions Here we present the characterization of the Populus tremula x alba LHY2 promoter under three different photoperiod conditions. Our results indicate the selected promoter region contains the circadian elements as well as the luciferase activity shows the expected expression under both long and short days. In the Y1H screening, we found several candidates that are classified either as transcription factors or chromatin remodelers. We will discuss the possible role of these proteins as regulators of the poplar circadian clock.

More information

Item ID: 42207
DC Identifier: http://oa.upm.es/42207/
OAI Identifier: oai:oa.upm.es:42207
Deposited by: Memoria Investigacion
Deposited on: 28 Oct 2016 12:52
Last Modified: 28 Oct 2016 12:52
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