New insights on winter-associated regulators of the circadian clock in poplar

Ramos Sanchez, Jose Manuel; Moreno Cortés, Alicia; Conde Rodriguez, Daniel; Hernández Verdeja, Tamara; Perales, Mariano y Allona Alberich, Isabel Marta (2015). New insights on winter-associated regulators of the circadian clock in poplar. En: "XXI Reunión de la Sociedad Española de Fisiología Vegetal", 14/06/2015-17/06/2015, Toledo (España). p. 1.

Descripción

Título: New insights on winter-associated regulators of the circadian clock in poplar
Autor/es:
  • Ramos Sanchez, Jose Manuel
  • Moreno Cortés, Alicia
  • Conde Rodriguez, Daniel
  • Hernández Verdeja, Tamara
  • Perales, Mariano
  • Allona Alberich, Isabel Marta
Tipo de Documento: Ponencia en Congreso o Jornada (Artículo)
Título del Evento: XXI Reunión de la Sociedad Española de Fisiología Vegetal
Fechas del Evento: 14/06/2015-17/06/2015
Lugar del Evento: Toledo (España)
Título del Libro: XXI Reunión de la Sociedad Española de Fisiología Vegetal
Fecha: 2015
Materias:
Escuela: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Departamento: Biotecnología - Biología Vegetal
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Winter dormancy is an adaptive mechanism that allows trees from temperate and cold regions to survive the harsh conditions of this season. This process is strongly influenced by daylength and temperature, which are signals that are integrated by the circadian clock. Low temperatures cause the disruption of the circadian clock in trees, which consists in a transcriptional activation followed by an arrhythmic expression. In this work we uncover winter-associated regulators of the circadian clock in poplar. We generated transgenic poplars that express a transcriptional fusion between the promoter of a circadian clock gene and the luciferase gene. Setting up a circadian luminiscence assay by which we register the luciferase activity from leaf discs with a luminometer, we have characterized the expression of this promoter under different photoperiods. Then, we carried out a Yeast One Hybrid (Y1H) screening with a library enriched in winter-associated factors using this promoter as bait. Candidate regulators are tested in vivo by transient assays in poplar, overexpressing and silencing the candidate genes. Our results indicate the selected promoter region contains the circadian elements as well as the luciferase activity shows the expected expression under both long and short days. In the Y1H screening, we found several candidates that are classified either as transcription factors or chromatin remodelers. We discuss the putative role of these proteins as regulators of the poplar circadian clock.

Más información

ID de Registro: 42209
Identificador DC: http://oa.upm.es/42209/
Identificador OAI: oai:oa.upm.es:42209
Depositado por: Memoria Investigacion
Depositado el: 12 Ene 2017 18:20
Ultima Modificación: 12 Ene 2017 18:20
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