New insights on winter-associated regulators of the circadian clock in poplar

Ramos Sanchez, Jose Manuel and Moreno Cortés, Alicia and Conde Rodriguez, Daniel and Hernández Verdeja, Tamara and Perales, Mariano and Allona Alberich, Isabel Marta (2015). New insights on winter-associated regulators of the circadian clock in poplar. In: "XXI Reunión de la Sociedad Española de Fisiología Vegetal", 14/06/2015-17/06/2015, Toledo (España). p. 1.

Description

Title: New insights on winter-associated regulators of the circadian clock in poplar
Author/s:
  • Ramos Sanchez, Jose Manuel
  • Moreno Cortés, Alicia
  • Conde Rodriguez, Daniel
  • Hernández Verdeja, Tamara
  • Perales, Mariano
  • Allona Alberich, Isabel Marta
Item Type: Presentation at Congress or Conference (Article)
Event Title: XXI Reunión de la Sociedad Española de Fisiología Vegetal
Event Dates: 14/06/2015-17/06/2015
Event Location: Toledo (España)
Title of Book: XXI Reunión de la Sociedad Española de Fisiología Vegetal
Date: 2015
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Winter dormancy is an adaptive mechanism that allows trees from temperate and cold regions to survive the harsh conditions of this season. This process is strongly influenced by daylength and temperature, which are signals that are integrated by the circadian clock. Low temperatures cause the disruption of the circadian clock in trees, which consists in a transcriptional activation followed by an arrhythmic expression. In this work we uncover winter-associated regulators of the circadian clock in poplar. We generated transgenic poplars that express a transcriptional fusion between the promoter of a circadian clock gene and the luciferase gene. Setting up a circadian luminiscence assay by which we register the luciferase activity from leaf discs with a luminometer, we have characterized the expression of this promoter under different photoperiods. Then, we carried out a Yeast One Hybrid (Y1H) screening with a library enriched in winter-associated factors using this promoter as bait. Candidate regulators are tested in vivo by transient assays in poplar, overexpressing and silencing the candidate genes. Our results indicate the selected promoter region contains the circadian elements as well as the luciferase activity shows the expected expression under both long and short days. In the Y1H screening, we found several candidates that are classified either as transcription factors or chromatin remodelers. We discuss the putative role of these proteins as regulators of the poplar circadian clock.

More information

Item ID: 42209
DC Identifier: http://oa.upm.es/42209/
OAI Identifier: oai:oa.upm.es:42209
Deposited by: Memoria Investigacion
Deposited on: 12 Jan 2017 18:20
Last Modified: 12 Jan 2017 18:20
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