Role of poplar DNA demethylases in controlling winter dormancy

Conde Rodriguez, Daniel and Moreno Cortés, Alicia and Hernández Verdeja, Tamara and Ramos Sanchez, Jose Manuel and Perales, Mariano and González Melendi, Pablo and Allona Alberich, Isabel Marta (2015). Role of poplar DNA demethylases in controlling winter dormancy. In: "XXI Reunión de la Sociedad Española de Fisiología Vegetal", 14/06/2015-17/06/2015, Toledo (España). ISBN 978-84-606-8883-9. p. 1.

Description

Title: Role of poplar DNA demethylases in controlling winter dormancy
Author/s:
  • Conde Rodriguez, Daniel
  • Moreno Cortés, Alicia
  • Hernández Verdeja, Tamara
  • Ramos Sanchez, Jose Manuel
  • Perales, Mariano
  • González Melendi, Pablo
  • Allona Alberich, Isabel Marta
Item Type: Presentation at Congress or Conference (Article)
Event Title: XXI Reunión de la Sociedad Española de Fisiología Vegetal
Event Dates: 14/06/2015-17/06/2015
Event Location: Toledo (España)
Title of Book: XXI Reunión de la Sociedad Española de Fisiología Vegetal y XIV Congreso Hispano-Luso de Fisiología Vegetal
Date: 2015
ISBN: 978-84-606-8883-9
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Trees have developed the mechanism of winter dormancy to survive the harsh conditions of winter in temperate and cold regions. It determines the geographical distribution of tree species and the long of the growing period. Epigenetic control of winter dormancy in woody plants is barely known. 5-methyl cytosine (5mC) is an important epigenetic mark that regulates gene expression in animals and plants. The profile of DNA methylation changes in the transition of developmental stages in plants such as chestnut bud set and burst, flowering in azalea, aging in pine trees among other. So far, the enzymes involved in the modification of the methylome and the molecular mechanism behind the control over those development processes remain to be understood. Our previous results showed higher DNA methylation and less acetylated Lys 8 of histone H4 global levels in poplar stem during winter dormancy compared to active growing season. Analysis of the 5-methyl cytosine levels by the application of the immunofluorescence-based method set up in our lab showed that global DNA methylation decrease significantly when trees are near to restore the growing season after the winter dormancy. We have identified two poplar homologs to Arabidopsis DME gene: PtaDML8/PtaDML10. DME protein promotes global DNA demethylation along the genome during the endosperm development. Our RT-PCR analyses indicate that the expression of PtaDML8/PtaDML10 genes increases significantly when trees are near to restart growing after the winter. Functional characterization showed that PtaDML8/PtaDML10 knockdown plants have a delayed in resuming of growth after dormancy. Taken together, we hypothesize that an active control of the 5mC DNA methylation play a key role in winter dormancy and that 5mC demethylases would be crucial for poplar dormant-growth transition

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGL2011-22625/FORUnspecifiedUnspecifiedUnspecified

More information

Item ID: 42262
DC Identifier: http://oa.upm.es/42262/
OAI Identifier: oai:oa.upm.es:42262
Deposited by: Memoria Investigacion
Deposited on: 02 Feb 2017 17:04
Last Modified: 02 Feb 2017 17:04
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