Towards understanding RAV1 transcriptional network during the growth-dormancy cycle in poplar

Triozzi, Paolo M. and Ramos Sanchez, Jose Manuel and Moreno Cortés, Alicia and Conde Rodriguez, Daniel and González Melendi, Pablo and Allona Alberich, Isabel Marta and Perales, Mariano (2015). Towards understanding RAV1 transcriptional network during the growth-dormancy cycle in poplar. In: "XXI Reunión de la Sociedad Española de Fisiología Vegetal", 14/06/2015-17/06/2015, Toledo (España). p. 1.

Description

Title: Towards understanding RAV1 transcriptional network during the growth-dormancy cycle in poplar
Author/s:
  • Triozzi, Paolo M.
  • Ramos Sanchez, Jose Manuel
  • Moreno Cortés, Alicia
  • Conde Rodriguez, Daniel
  • González Melendi, Pablo
  • Allona Alberich, Isabel Marta
  • Perales, Mariano
Item Type: Presentation at Congress or Conference (Article)
Event Title: XXI Reunión de la Sociedad Española de Fisiología Vegetal
Event Dates: 14/06/2015-17/06/2015
Event Location: Toledo (España)
Title of Book: XXI Reunión de la Sociedad Española de Fisiología Vegetal
Date: 2015
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

As plants are sessile organisms, the decision to grow or to stop growing is fundamental for their survival. To survive to the harsh condition of the winter, temperate and boreal trees undergo a self-protective developmental reprograming known as winter dormancy. Its mechanism involves an intricate interplay between endogenous growth regulators and the winter external signal, such as shortening of photoperiod and cold temperatures. Currently, only few factors including circadian and developmental transcription factors and hormonal signaling molecules have been shown to modulate the extension of the dormancy period in woody plants. In our laboratory, the circadian controlled transcription factor RAV promotes sylleptic branching and reduces dormancy length when RAV is ectopic overexpressed in poplar (1). Comparative mRNA profiling of RAV overexpressing versus wild type revealed a transcriptional rearrangement happens as effect of constitutive high level of RAV, giving rise to phenology. However, to define RAV mode of action as a transcriptional regulator and how RAV activity trigger a modification of dormancy-growth cycle, its primary targets genes should be investigated. At this particular, the translational fusion of a given transcription factor to the rat glucorticoid receptor domain (GR) have been widely used in Arabidopsis and recently in poplar (2). In this work we show the standardization of a methodology required to identify primary target genes for a transcription factor in poplar. This will be useful to future exploration of RAV1 gene network and its mode of action in spatio-temporal studies at tissue level during growth-dormancy cycle in poplar.

More information

Item ID: 42280
DC Identifier: http://oa.upm.es/42280/
OAI Identifier: oai:oa.upm.es:42280
Deposited by: Memoria Investigacion
Deposited on: 12 Jan 2017 18:15
Last Modified: 12 Jan 2017 18:15
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