Towards understanding RAV1 transcriptional network during the growth-dormancy cycle in poplar

Triozzi, Paolo M.; Ramos Sanchez, Jose Manuel; Moreno Cortés, Alicia; Conde Rodriguez, Daniel; González Melendi, Pablo; Allona Alberich, Isabel Marta y Perales, Mariano (2015). Towards understanding RAV1 transcriptional network during the growth-dormancy cycle in poplar. En: "XXI Reunión de la Sociedad Española de Fisiología Vegetal", 14/06/2015-17/06/2015, Toledo (España). p. 1.

Descripción

Título: Towards understanding RAV1 transcriptional network during the growth-dormancy cycle in poplar
Autor/es:
  • Triozzi, Paolo M.
  • Ramos Sanchez, Jose Manuel
  • Moreno Cortés, Alicia
  • Conde Rodriguez, Daniel
  • González Melendi, Pablo
  • Allona Alberich, Isabel Marta
  • Perales, Mariano
Tipo de Documento: Ponencia en Congreso o Jornada (Artículo)
Título del Evento: XXI Reunión de la Sociedad Española de Fisiología Vegetal
Fechas del Evento: 14/06/2015-17/06/2015
Lugar del Evento: Toledo (España)
Título del Libro: XXI Reunión de la Sociedad Española de Fisiología Vegetal
Fecha: 2015
Materias:
Escuela: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Departamento: Biotecnología - Biología Vegetal
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

As plants are sessile organisms, the decision to grow or to stop growing is fundamental for their survival. To survive to the harsh condition of the winter, temperate and boreal trees undergo a self-protective developmental reprograming known as winter dormancy. Its mechanism involves an intricate interplay between endogenous growth regulators and the winter external signal, such as shortening of photoperiod and cold temperatures. Currently, only few factors including circadian and developmental transcription factors and hormonal signaling molecules have been shown to modulate the extension of the dormancy period in woody plants. In our laboratory, the circadian controlled transcription factor RAV promotes sylleptic branching and reduces dormancy length when RAV is ectopic overexpressed in poplar (1). Comparative mRNA profiling of RAV overexpressing versus wild type revealed a transcriptional rearrangement happens as effect of constitutive high level of RAV, giving rise to phenology. However, to define RAV mode of action as a transcriptional regulator and how RAV activity trigger a modification of dormancy-growth cycle, its primary targets genes should be investigated. At this particular, the translational fusion of a given transcription factor to the rat glucorticoid receptor domain (GR) have been widely used in Arabidopsis and recently in poplar (2). In this work we show the standardization of a methodology required to identify primary target genes for a transcription factor in poplar. This will be useful to future exploration of RAV1 gene network and its mode of action in spatio-temporal studies at tissue level during growth-dormancy cycle in poplar.

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ID de Registro: 42280
Identificador DC: http://oa.upm.es/42280/
Identificador OAI: oai:oa.upm.es:42280
Depositado por: Memoria Investigacion
Depositado el: 12 Ene 2017 18:15
Ultima Modificación: 12 Ene 2017 18:15
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