Understanding the role of 5-Methyl cytosine DNA demethylases in controlling winter dormacy of woody plants

Conde Rodriguez, Daniel; Moreno Cortes, Alicia; Hernandez Verdeja, Tamara; Perales, Mariano; González Melendi, Pablo y Allona Alberich, Isabel Marta (2015). Understanding the role of 5-Methyl cytosine DNA demethylases in controlling winter dormacy of woody plants. En: "4th European Workshop on Plant Chromatin", 25/06/2015-26/06/2015, Uppsala, Sweden. p. 1.

Descripción

Título: Understanding the role of 5-Methyl cytosine DNA demethylases in controlling winter dormacy of woody plants
Autor/es:
  • Conde Rodriguez, Daniel
  • Moreno Cortes, Alicia
  • Hernandez Verdeja, Tamara
  • Perales, Mariano
  • González Melendi, Pablo
  • Allona Alberich, Isabel Marta
Tipo de Documento: Ponencia en Congreso o Jornada (Artículo)
Título del Evento: 4th European Workshop on Plant Chromatin
Fechas del Evento: 25/06/2015-26/06/2015
Lugar del Evento: Uppsala, Sweden
Título del Libro: 4th European Workshop on Plant Chromatin
Fecha: 2015
Materias:
Escuela: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Departamento: Biotecnología - Biología Vegetal
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Winter dormancy is the mechanism used by perennial plants to survive the harsh conditions of winter in temperate and cold regions and determines the geographical distribution of tree species (Chuine and Beaubien 2001; Horvath et al. 2003; Allona et al. 2008). Epigenetic control of winter dormancy in woody plants is barely known. Among the important epigenetic marks, 5-methyl cytosine (5mC) regulates gene expression in animals and plants. Global changes in 5mC DNA methylation have been shown in the transition of developmental stages in plants such as chestnut bud set and burst, flowering in azalea, aging in pine trees among other. However, the mechanism and the enzymes involved in the modification of the methylome and its control over those development processes remain to be identified. Our previous results showed higher DNA methylation and less acetylated Lys 8 of histone H4 global levels in poplar stem during winter dormancy compared to active growing season (Conde et al. 2013). Analysis of the 5-methyl cytosine levels by the application of the immunofluorescece-based method set up in our lab showed that DNA methylation leves fall suddenly when trees are near to restore the growing season coming from the dormant state. We have identified two poplar homologs to Arabidopsis DME gene: PtaDML8/PtaDML10. DME protein promotes global DNA demethylation along the genome during the endosperm development. Our RT-PCR analyses indicate that the expression of PtaDML8/PtaDML10 genes increases significantly when trees are near to restart growing after winter dormancy. The phenologycal assays showed that PtaDML8/PtaDML10 knockdown plants have a delayed in resuming of growth after dormancy. Taken together, we hypothesize that an active control of the 5mC DNA methylation might play a key role in winter dormancy and that 5mC demethylases would be crucial in this process.

Más información

ID de Registro: 42281
Identificador DC: http://oa.upm.es/42281/
Identificador OAI: oai:oa.upm.es:42281
URL Oficial: https://colloque6.inra.fr/ewpc_series_on_plant_chromatin
Depositado por: Memoria Investigacion
Depositado el: 25 Oct 2016 17:35
Ultima Modificación: 25 Oct 2016 17:35
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