Effect of antioxidants on the genetic stability of cryopreserved mint shoot tips by encapsulation-dehydration

González Benito, Elena; Kremer Morales, María Carolina; Ibáñez Ruiz, Miguel Ángel y Martín Fernández, Carmen (2016). Effect of antioxidants on the genetic stability of cryopreserved mint shoot tips by encapsulation-dehydration. "Plant Cell Tissue Orga", v. 127 (n. 2); pp. 359-368. ISSN 0167-6857. https://doi.org/10.1007/s11240-016-1056-8.

Descripción

Título: Effect of antioxidants on the genetic stability of cryopreserved mint shoot tips by encapsulation-dehydration
Autor/es:
  • González Benito, Elena
  • Kremer Morales, María Carolina
  • Ibáñez Ruiz, Miguel Ángel
  • Martín Fernández, Carmen
Tipo de Documento: Artículo
Título de Revista/Publicación: Plant Cell Tissue Orga
Fecha: Noviembre 2016
Volumen: 127
Materias:
Escuela: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Departamento: Biotecnología - Biología Vegetal
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

The effect of antioxidants applied in one step of a cryopreservation protocol by encapsulation?dehydration on recovery and genetic stability of mint shoot tips has been studied. Glutathione (0.16 or 0.24 mM), ascorbic acid (0.28 or 0.43 mM) and ?-tocopherol (vitamin E) were added to the preculture medium (0.3 M sucrose). DNA was extracted from three different types of samples: leaves from shoots, callus at the base of shoots and callus. RAPD and AFLP markers were used to assess the genetic stability. The use of antioxidants did not improve recovery after cryopreservation. One of the genotypes, ?MEN 198?, showed higher percentage of stable samples than the other one, ?MEN 186? (56 vs. 37 %; considering all treatments and types of explant). The use of vitamin E improved the percentage of stable samples with respect to control treatment (no antioxidant) in ?MEN 186?. No differences in the percentages of stable samples were observed among cryopreserved and non-cryopreserved (treated similarly without immersion in liquid nitrogen) plant material. Recovered shoots of both genotypes showed higher stability (76?80 % stable samples) than callus samples (14?22 %).

Más información

ID de Registro: 44279
Identificador DC: http://oa.upm.es/44279/
Identificador OAI: oai:oa.upm.es:44279
Identificador DOI: 10.1007/s11240-016-1056-8
URL Oficial: http://link.springer.com/article/10.1007/s11240-016-1056-8
Depositado por: Memoria Investigacion
Depositado el: 11 Ene 2017 15:54
Ultima Modificación: 30 Nov 2017 23:30
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