Effect of antioxidants on the genetic stability of cryopreserved mint shoot tips by encapsulation-dehydration

González Benito, Elena and Kremer Morales, María Carolina and Ibáñez Ruiz, Miguel Ángel and Martín Fernández, Carmen (2016). Effect of antioxidants on the genetic stability of cryopreserved mint shoot tips by encapsulation-dehydration. "Plant Cell Tissue Orga", v. 127 (n. 2); pp. 359-368. ISSN 0167-6857. https://doi.org/10.1007/s11240-016-1056-8.

Description

Title: Effect of antioxidants on the genetic stability of cryopreserved mint shoot tips by encapsulation-dehydration
Author/s:
  • González Benito, Elena
  • Kremer Morales, María Carolina
  • Ibáñez Ruiz, Miguel Ángel
  • Martín Fernández, Carmen
Item Type: Article
Título de Revista/Publicación: Plant Cell Tissue Orga
Date: November 2016
ISSN: 0167-6857
Volume: 127
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (1MB) | Preview

Abstract

The effect of antioxidants applied in one step of a cryopreservation protocol by encapsulation?dehydration on recovery and genetic stability of mint shoot tips has been studied. Glutathione (0.16 or 0.24 mM), ascorbic acid (0.28 or 0.43 mM) and ?-tocopherol (vitamin E) were added to the preculture medium (0.3 M sucrose). DNA was extracted from three different types of samples: leaves from shoots, callus at the base of shoots and callus. RAPD and AFLP markers were used to assess the genetic stability. The use of antioxidants did not improve recovery after cryopreservation. One of the genotypes, ?MEN 198?, showed higher percentage of stable samples than the other one, ?MEN 186? (56 vs. 37 %; considering all treatments and types of explant). The use of vitamin E improved the percentage of stable samples with respect to control treatment (no antioxidant) in ?MEN 186?. No differences in the percentages of stable samples were observed among cryopreserved and non-cryopreserved (treated similarly without immersion in liquid nitrogen) plant material. Recovered shoots of both genotypes showed higher stability (76?80 % stable samples) than callus samples (14?22 %).

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGL2010-21989-C02-01UnspecifiedUnspecifiedEstudios sobre los procesos de crioconservación en menta

More information

Item ID: 44279
DC Identifier: http://oa.upm.es/44279/
OAI Identifier: oai:oa.upm.es:44279
DOI: 10.1007/s11240-016-1056-8
Official URL: http://link.springer.com/article/10.1007/s11240-016-1056-8
Deposited by: Memoria Investigacion
Deposited on: 11 Jan 2017 15:54
Last Modified: 18 Mar 2019 15:33
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM