Arabidopsis NITRILASE 1 contributes to the regulation of root growth and development through modulation of auxin biosynthesis in seedlings

Lehmann, Thomas and Janowitz, TiM and Sánchez Parra, Beatriz and Pérez Alonso, Marta Marina and Trompetter, Inga and Piotrowski, Markus and Pollmann, Stephan (2017). Arabidopsis NITRILASE 1 contributes to the regulation of root growth and development through modulation of auxin biosynthesis in seedlings. "Frontiers in Plant Science", v. 8 (n. 36); pp. 1-15. ISSN 1664-462X. https://doi.org/10.3389/fpls.2017.00036.

Description

Title: Arabidopsis NITRILASE 1 contributes to the regulation of root growth and development through modulation of auxin biosynthesis in seedlings
Author/s:
  • Lehmann, Thomas
  • Janowitz, TiM
  • Sánchez Parra, Beatriz
  • Pérez Alonso, Marta Marina
  • Trompetter, Inga
  • Piotrowski, Markus
  • Pollmann, Stephan
Item Type: Article
Título de Revista/Publicación: Frontiers in Plant Science
Date: January 2017
ISSN: 1664-462X
Volume: 8
Subjects:
Freetext Keywords: Arabidopsis thaliana, auxin biosynthesis, nitrilases, indole-3-acetic acid, root development, GC-MS/MS
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Nitrilases consist of a group of enzymes that catalyze the hydrolysis of organic cyanides. They are found ubiquitously distributed in the plant kingdom. Plant nitrilases are mainly involved in the detoxification of ß-cyanoalanine, a side-product of ethylene biosynthesis. In the model plant Arabidopsis thaliana a second group of Brassicaceae-specific nitrilases (NIT1-3) has been found. This so-called NIT1-subfamily has been associated with the conversion of indole-3-acetonitrile (IAN) into the major plant growth hormone, indole-3-acetic acid (IAA). However, apart of reported functions in defense responses to pathogens and in responses to sulfur depletion, conclusive insight into the general physiological function of the NIT-subfamily nitrilases remains elusive. In this report, we test the influence of altered nitrilase expression on plant development. We took a genetic approach to disclose the function of NITRILASE 1 (NIT1) of A. thaliana. We show that NIT over-expression (NIT1ox) results in seedlings with shorter primary roots, and an increased number of lateral roots. In addition, NIT1ox plants exhibit drastic changes of both free IAA and IAN levels, which are suggested to be the reason for the observed phenotype. On the other hand, NIT2RNAi knockdown lines, capable of suppressing the expression of all members of the NIT1-subfamily, were generated and characterized to substantiate the abovementioned findings. Our results demonstrate for the first time that Arabidopsis NIT1 has profound effects on root morphogenesis in early seedling development.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainSP: SFB480/A10UnspecifiedUnspecifiedUnspecified
Government of SpainBFU-2014-55575-RUnspecifiedUnspecifiedUnspecified
Government of SpainMP: SPP1152, PI424/2-1UnspecifiedUnspecifiedUnspecified

More information

Item ID: 45019
DC Identifier: http://oa.upm.es/45019/
OAI Identifier: oai:oa.upm.es:45019
DOI: 10.3389/fpls.2017.00036
Official URL: http://journal.frontiersin.org/article/10.3389/fpls.2017.00036/full
Deposited by: Memoria Investigacion
Deposited on: 04 Apr 2017 16:30
Last Modified: 04 Apr 2017 16:30
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