Arabidopsis NITRILASE 1 contributes to the regulation of root growth and development through modulation of auxin biosynthesis in seedlings

Lehmann, Thomas; Janowitz, TiM; Sánchez Parra, Beatriz; Pérez Alonso, Marta Marina; Trompetter, Inga; Piotrowski, Markus y Pollmann, Stephan (2017). Arabidopsis NITRILASE 1 contributes to the regulation of root growth and development through modulation of auxin biosynthesis in seedlings. "Frontiers in Plant Science", v. 8 (n. 36); pp. 1-15. ISSN 1664-462X. https://doi.org/10.3389/fpls.2017.00036.

Descripción

Título: Arabidopsis NITRILASE 1 contributes to the regulation of root growth and development through modulation of auxin biosynthesis in seedlings
Autor/es:
  • Lehmann, Thomas
  • Janowitz, TiM
  • Sánchez Parra, Beatriz
  • Pérez Alonso, Marta Marina
  • Trompetter, Inga
  • Piotrowski, Markus
  • Pollmann, Stephan
Tipo de Documento: Artículo
Título de Revista/Publicación: Frontiers in Plant Science
Fecha: Enero 2017
Volumen: 8
Materias:
Palabras Clave Informales: Arabidopsis thaliana, auxin biosynthesis, nitrilases, indole-3-acetic acid, root development, GC-MS/MS
Escuela: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Departamento: Biotecnología - Biología Vegetal
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

Texto completo

[img]
Vista Previa
PDF (Document Portable Format) - Se necesita un visor de ficheros PDF, como GSview, Xpdf o Adobe Acrobat Reader
Descargar (1MB) | Vista Previa

Resumen

Nitrilases consist of a group of enzymes that catalyze the hydrolysis of organic cyanides. They are found ubiquitously distributed in the plant kingdom. Plant nitrilases are mainly involved in the detoxification of ß-cyanoalanine, a side-product of ethylene biosynthesis. In the model plant Arabidopsis thaliana a second group of Brassicaceae-specific nitrilases (NIT1-3) has been found. This so-called NIT1-subfamily has been associated with the conversion of indole-3-acetonitrile (IAN) into the major plant growth hormone, indole-3-acetic acid (IAA). However, apart of reported functions in defense responses to pathogens and in responses to sulfur depletion, conclusive insight into the general physiological function of the NIT-subfamily nitrilases remains elusive. In this report, we test the influence of altered nitrilase expression on plant development. We took a genetic approach to disclose the function of NITRILASE 1 (NIT1) of A. thaliana. We show that NIT over-expression (NIT1ox) results in seedlings with shorter primary roots, and an increased number of lateral roots. In addition, NIT1ox plants exhibit drastic changes of both free IAA and IAN levels, which are suggested to be the reason for the observed phenotype. On the other hand, NIT2RNAi knockdown lines, capable of suppressing the expression of all members of the NIT1-subfamily, were generated and characterized to substantiate the abovementioned findings. Our results demonstrate for the first time that Arabidopsis NIT1 has profound effects on root morphogenesis in early seedling development.

Proyectos asociados

TipoCódigoAcrónimoResponsableTítulo
Gobierno de EspañaSP: SFB480/A10Sin especificarSin especificarSin especificar
Gobierno de EspañaBFU-2014-55575-RSin especificarSin especificarSin especificar
Gobierno de EspañaMP: SPP1152, PI424/2-1Sin especificarSin especificarSin especificar

Más información

ID de Registro: 45019
Identificador DC: http://oa.upm.es/45019/
Identificador OAI: oai:oa.upm.es:45019
Identificador DOI [BETA]: 10.3389/fpls.2017.00036
URL Oficial: http://journal.frontiersin.org/article/10.3389/fpls.2017.00036/full
Depositado por: Memoria Investigacion
Depositado el: 04 Abr 2017 16:30
Ultima Modificación: 04 Abr 2017 16:30
  • Open Access
  • Open Access
  • Sherpa-Romeo
    Compruebe si la revista anglosajona en la que ha publicado un artículo permite también su publicación en abierto.
  • Dulcinea
    Compruebe si la revista española en la que ha publicado un artículo permite también su publicación en abierto.
  • Recolecta
  • e-ciencia
  • Observatorio I+D+i UPM
  • OpenCourseWare UPM