In vivo and in vitro maturation of rabbit oocyte affects gene expression, mitochondrial distribution and apoptosis

Arias Álvarez, María; García García, R. M.; López Tello, J.; García Rebollar, Pilar; Gutiérrez Adán, A. y Lorenzo, P.L. (2016). In vivo and in vitro maturation of rabbit oocyte affects gene expression, mitochondrial distribution and apoptosis. "Reproduction, Fertility, And Development" ; pp. 1-13. ISSN 1031-3613. https://doi.org/10.1071/RD15553.

Descripción

Título: In vivo and in vitro maturation of rabbit oocyte affects gene expression, mitochondrial distribution and apoptosis
Autor/es:
  • Arias Álvarez, María
  • García García, R. M.
  • López Tello, J.
  • García Rebollar, Pilar
  • Gutiérrez Adán, A.
  • Lorenzo, P.L.
Tipo de Documento: Artículo
Título de Revista/Publicación: Reproduction, Fertility, And Development
Fecha: Septiembre 2016
Materias:
Escuela: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Departamento: Producción Agraria
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

In vivo-matured cumulus–oocyte complexes are valuable models in which to assess potential biomarkers of rabbit oocyte quality that contribute to enhanced IVM systems. In the present study we compared some gene markers of oocytes and cumulus cells (CCs) from immature, in vivo-matured and IVM oocytes. Moreover, apoptosis in CCs, nuclear maturation, mitochondrial reallocation and the developmental potential of oocytes after IVF were assessed. In relation to cumulus expansion, gene expression of gap junction protein, alpha 1, 43 kDa (Gja1) and prostaglandin-endoperoxide synthase 2 (Ptgs2) was significantly lower in CCs after in vivo maturation than IVM. In addition, there were differences in gene expression after in vivo maturation versus IVM in both oocytes and CCs for genes related to cell cycle regulation and apoptosis (V-Akt murine thymoma viral oncogene homologue 1 (Akt1), tumour protein 53 (Tp53), caspase 3, apoptosis-related cysteine protease (Casp3)), oxidative response (superoxide dismutase 2, mitochondrial (Sod2)) and metabolism (glucose-6-phosphate dehydrogenase (G6pd), glyceraldehyde-3-phosphate dehydrogenase (Gapdh)). In vivo-matured CCs had a lower apoptosis rate than IVM and immature CCs. Meiotic progression, mitochondrial migration to the periphery and developmental competence were higher for in vivo-matured than IVM oocytes. In conclusion, differences in oocyte developmental capacity after IVM or in vivo maturation are accompanied by significant changes in transcript abundance in oocytes and their surrounding CCs, meiotic rate, mitochondrial distribution and apoptotic index. Some of the genes investigated, such as Gja1, could be potential biomarkers for oocyte developmental competence in the rabbit model, helping improve in vitro culture systems in these species.

Más información

ID de Registro: 45755
Identificador DC: http://oa.upm.es/45755/
Identificador OAI: oai:oa.upm.es:45755
Identificador DOI: 10.1071/RD15553
URL Oficial: http://www.publish.csiro.au/RD/RD15553
Depositado por: Memoria Investigacion
Depositado el: 22 May 2017 15:33
Ultima Modificación: 22 May 2017 15:33
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