In vivo and in vitro maturation of rabbit oocyte affects gene expression, mitochondrial distribution and apoptosis

Arias Álvarez, María and García García, R. M. and López Tello, J. and García Rebollar, Pilar and Gutiérrez Adán, A. and Lorenzo, P.L. (2016). In vivo and in vitro maturation of rabbit oocyte affects gene expression, mitochondrial distribution and apoptosis. "Reproduction, Fertility, And Development" ; pp. 1-13. ISSN 1031-3613. https://doi.org/10.1071/RD15553.

Description

Title: In vivo and in vitro maturation of rabbit oocyte affects gene expression, mitochondrial distribution and apoptosis
Author/s:
  • Arias Álvarez, María
  • García García, R. M.
  • López Tello, J.
  • García Rebollar, Pilar
  • Gutiérrez Adán, A.
  • Lorenzo, P.L.
Item Type: Article
Título de Revista/Publicación: Reproduction, Fertility, And Development
Date: September 2016
ISSN: 1031-3613
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Producción Agraria
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

In vivo-matured cumulus–oocyte complexes are valuable models in which to assess potential biomarkers of rabbit oocyte quality that contribute to enhanced IVM systems. In the present study we compared some gene markers of oocytes and cumulus cells (CCs) from immature, in vivo-matured and IVM oocytes. Moreover, apoptosis in CCs, nuclear maturation, mitochondrial reallocation and the developmental potential of oocytes after IVF were assessed. In relation to cumulus expansion, gene expression of gap junction protein, alpha 1, 43 kDa (Gja1) and prostaglandin-endoperoxide synthase 2 (Ptgs2) was significantly lower in CCs after in vivo maturation than IVM. In addition, there were differences in gene expression after in vivo maturation versus IVM in both oocytes and CCs for genes related to cell cycle regulation and apoptosis (V-Akt murine thymoma viral oncogene homologue 1 (Akt1), tumour protein 53 (Tp53), caspase 3, apoptosis-related cysteine protease (Casp3)), oxidative response (superoxide dismutase 2, mitochondrial (Sod2)) and metabolism (glucose-6-phosphate dehydrogenase (G6pd), glyceraldehyde-3-phosphate dehydrogenase (Gapdh)). In vivo-matured CCs had a lower apoptosis rate than IVM and immature CCs. Meiotic progression, mitochondrial migration to the periphery and developmental competence were higher for in vivo-matured than IVM oocytes. In conclusion, differences in oocyte developmental capacity after IVM or in vivo maturation are accompanied by significant changes in transcript abundance in oocytes and their surrounding CCs, meiotic rate, mitochondrial distribution and apoptotic index. Some of the genes investigated, such as Gja1, could be potential biomarkers for oocyte developmental competence in the rabbit model, helping improve in vitro culture systems in these species.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGL-2011-23822UnspecifiedUnspecifiedUnspecified
Government of SpainAGL2015-65572UnspecifiedUnspecifiedUnspecified
Government of SpainAGL2015-66145-RUnspecifiedUnspecifiedUnspecified
Madrid Regional GovernmentS2013/ABI-2913UnspecifiedUnspecifiedUnspecified

More information

Item ID: 45755
DC Identifier: http://oa.upm.es/45755/
OAI Identifier: oai:oa.upm.es:45755
DOI: 10.1071/RD15553
Official URL: http://www.publish.csiro.au/RD/RD15553
Deposited by: Memoria Investigacion
Deposited on: 22 May 2017 15:33
Last Modified: 19 Mar 2019 15:51
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