Monitoring of airborne biological particles in outdoor atmosphere. Part 2: Metagenomics applied to urban environments

Núñez, Andrés; Amo de Paz, Guillermo; Rastrojo, Alberto; García Ruiz, Ana María; Alcamí, Antonio; Gutiérrez-Bustillo, A. Montserrat y Moreno Gómez, Diego Alejandro (2016). Monitoring of airborne biological particles in outdoor atmosphere. Part 2: Metagenomics applied to urban environments. "International Microbiology", v. 19 ; pp. 69-80. ISSN 1139-6709 e-ISSN: 1618-1095. https://doi.org/10.2436/20.1501.01.265.

Descripción

Título: Monitoring of airborne biological particles in outdoor atmosphere. Part 2: Metagenomics applied to urban environments
Autor/es:
  • Núñez, Andrés
  • Amo de Paz, Guillermo
  • Rastrojo, Alberto
  • García Ruiz, Ana María
  • Alcamí, Antonio
  • Gutiérrez-Bustillo, A. Montserrat
  • Moreno Gómez, Diego Alejandro
Tipo de Documento: Artículo
Título de Revista/Publicación: International Microbiology
Fecha: Abril 2016
Volumen: 19
Materias:
Palabras Clave Informales: airborne biological particles, metagenomics, next-generation sequencing (NGS), air biomonitoring, urban aerobiology
Escuela: E.T.S.I. Industriales (UPM)
Departamento: Física Aplicada e Ingeniería de Materiales
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

The air we breathe contains microscopic biological particles such as viruses, bacteria, fungi and pollen, some of them with relevant clinic importance. These organisms and/or their propagules have been traditionally studied by different disciplines and diverse methodologies like culture and microscopy. These techniques require time, expertise and also have some important biases. As a consequence, our knowledge on the total diversity and the relationships between the different biological entities present in the air is far from being complete. Currently, metagenomics and next-generation sequencing (NGS) may resolve this shortage of information and have been recently applied to metropolitan areas. Although the procedures and methods are not totally standardized yet, the first studies from urban air samples confirm the previous results obtained by culture and microscopy regarding abundance and variation of these biological particles. However, DNA-sequence analyses call into question some preceding ideas and also provide new interesting insights into diversity and their spatial distribution inside the cities. Here, we review the procedures, results and perspectives of the recent works that apply NGS to study the main biological particles present in the air of urban environments.

Proyectos asociados

TipoCódigoAcrónimoResponsableTítulo
Comunidad de MadridS2013/MAE-2874Sin especificarSin especificarAIRBIOTA-CM Program

Más información

ID de Registro: 45939
Identificador DC: http://oa.upm.es/45939/
Identificador OAI: oai:oa.upm.es:45939
Identificador DOI: 10.2436/20.1501.01.265
URL Oficial: http://revistes.iec.cat/index.php/IM/article/viewFile/142234/141150
Depositado por: Memoria Investigacion
Depositado el: 19 May 2017 09:34
Ultima Modificación: 19 May 2017 09:40
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