Monitoring of airborne biological particles in outdoor atmosphere. Part 2: Metagenomics applied to urban environments

Núñez, Andrés and Amo de Paz, Guillermo and Rastrojo, Alberto and García Ruiz, Ana María and Alcamí, Antonio and Gutiérrez-Bustillo, A. Montserrat and Moreno Gómez, Diego Alejandro (2016). Monitoring of airborne biological particles in outdoor atmosphere. Part 2: Metagenomics applied to urban environments. "International Microbiology", v. 19 ; pp. 69-80. ISSN 1139-6709 e-ISSN: 1618-1095. https://doi.org/10.2436/20.1501.01.265.

Description

Title: Monitoring of airborne biological particles in outdoor atmosphere. Part 2: Metagenomics applied to urban environments
Author/s:
  • Núñez, Andrés
  • Amo de Paz, Guillermo
  • Rastrojo, Alberto
  • García Ruiz, Ana María
  • Alcamí, Antonio
  • Gutiérrez-Bustillo, A. Montserrat
  • Moreno Gómez, Diego Alejandro
Item Type: Article
Título de Revista/Publicación: International Microbiology
Date: April 2016
ISSN: 1139-6709 e-ISSN: 1618-1095
Volume: 19
Subjects:
Freetext Keywords: airborne biological particles, metagenomics, next-generation sequencing (NGS), air biomonitoring, urban aerobiology
Faculty: E.T.S.I. Industriales (UPM)
Department: Física Aplicada e Ingeniería de Materiales
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The air we breathe contains microscopic biological particles such as viruses, bacteria, fungi and pollen, some of them with relevant clinic importance. These organisms and/or their propagules have been traditionally studied by different disciplines and diverse methodologies like culture and microscopy. These techniques require time, expertise and also have some important biases. As a consequence, our knowledge on the total diversity and the relationships between the different biological entities present in the air is far from being complete. Currently, metagenomics and next-generation sequencing (NGS) may resolve this shortage of information and have been recently applied to metropolitan areas. Although the procedures and methods are not totally standardized yet, the first studies from urban air samples confirm the previous results obtained by culture and microscopy regarding abundance and variation of these biological particles. However, DNA-sequence analyses call into question some preceding ideas and also provide new interesting insights into diversity and their spatial distribution inside the cities. Here, we review the procedures, results and perspectives of the recent works that apply NGS to study the main biological particles present in the air of urban environments.

Funding Projects

TypeCodeAcronymLeaderTitle
Madrid Regional GovernmentS2013/MAE-2874AIRBIOTADiego Alejandro Moreno GómezConocer y modelizar la contaminación biológica del aire urbano

More information

Item ID: 45939
DC Identifier: http://oa.upm.es/45939/
OAI Identifier: oai:oa.upm.es:45939
DOI: 10.2436/20.1501.01.265
Official URL: http://revistes.iec.cat/index.php/IM/article/viewFile/142234/141150
Deposited by: Memoria Investigacion
Deposited on: 19 May 2017 09:34
Last Modified: 20 Mar 2019 14:45
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