Influence of processing method of rumen contents on microbial populations in the inoculum and in vitro fermentation of substrates of variable composition

Mateos, I.; Ranilla, M. J.; Ramos, M.; Saro, C. y Carro Travieso, Maria Dolores (2016). Influence of processing method of rumen contents on microbial populations in the inoculum and in vitro fermentation of substrates of variable composition. "Animal Feed Science And Technology" (n. 220); pp. 109-120. ISSN 0377-8401. https://doi.org/10.1016/j.anifeedsci.2016.07.017.

Descripción

Título: Influence of processing method of rumen contents on microbial populations in the inoculum and in vitro fermentation of substrates of variable composition
Autor/es:
  • Mateos, I.
  • Ranilla, M. J.
  • Ramos, M.
  • Saro, C.
  • Carro Travieso, Maria Dolores
Tipo de Documento: Artículo
Título de Revista/Publicación: Animal Feed Science And Technology
Fecha: Octubre 2016
Materias:
Escuela: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Departamento: Producción Agraria
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

The in vitro batch culture technique is being increasingly used to study rumen fermentation, but the results are affected by several factors, being the source of the inoculum one of the most relevant. This work was conducted to assess the effects of different processing methods of ruminal contents on microbial populations in the obtained fluid, and its influence on fermentation parameters when the fluid was used as inoculum for in vitro incubations. Rumen contents were obtained from four rumen-fistulated sheep fed a 2:1 alfalfa hay:concentrate diet and subjected to the following treatments: SQ: squeezed through four layers of cheesecloth; FL: SQ treatment and further filtration through a 100-μm nylon cloth; STO: blended for three min at 230 rev min−1 in a Stomacher® and further filtrations as in SQ. Microbial populations’ abundance and bacterial diversity in the ruminal fluids were analysed by quantitative PCR (qPCR) and automated ribosomal intergenic spacer analysis (ARISA), respectively. Three forages (alfalfa hay, grass hay and barley straw) were incubated in vitro, either alone or mixed with concentrate (1:1), using each of the ruminal fluids as inoculum. There were no differences between SQ and FL methods in any of the microbial populations analysed, but STO increased the relative abundance of Fibrobacter succinogenes and Ruminococcus albus (P < 0.05) and decreased (P < 0.05) the concentration of protozoal DNA compared with SQ. Bacterial diversity was not affected (P > 0.05) by the processing methods. There were no interactions (P > 0.05) between the processing method and the characteristics of the substrates for any of the fermentation parameters analysed. Compared with SQ, the STO method resulted in greater (P < 0.05) methane production and ammonia-N concentrations in 8 h incubations. After 24 h of incubation, the use of STO inoculum increased (P < 0.05) methane production and dry matter degradability compared with SQ, with no differences in the rest of the parameters. No differences between SQ and FL methods were detected in any parameter. The results show that stomaching the rumen contents prior to inoculation of in vitro cultures modified some microbial populations, but had only subtle effects on fermentation parameters.

Más información

ID de Registro: 46101
Identificador DC: http://oa.upm.es/46101/
Identificador OAI: oai:oa.upm.es:46101
Identificador DOI: 10.1016/j.anifeedsci.2016.07.017
URL Oficial: http://www.sciencedirect.com/science/article/pii/S0377840116304412
Depositado por: Memoria Investigacion
Depositado el: 02 Jun 2017 15:41
Ultima Modificación: 30 Oct 2017 23:30
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