Influence of processing method of rumen contents on microbial populations in the inoculum and in vitro fermentation of substrates of variable composition

Mateos, I. and Ranilla, M. J. and Ramos, M. and Saro, C. and Carro Travieso, Maria Dolores (2016). Influence of processing method of rumen contents on microbial populations in the inoculum and in vitro fermentation of substrates of variable composition. "Animal Feed Science And Technology" (n. 220); pp. 109-120. ISSN 0377-8401. https://doi.org/10.1016/j.anifeedsci.2016.07.017.

Description

Title: Influence of processing method of rumen contents on microbial populations in the inoculum and in vitro fermentation of substrates of variable composition
Author/s:
  • Mateos, I.
  • Ranilla, M. J.
  • Ramos, M.
  • Saro, C.
  • Carro Travieso, Maria Dolores
Item Type: Article
Título de Revista/Publicación: Animal Feed Science And Technology
Date: October 2016
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Producción Agraria
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (870kB) | Preview

Abstract

The in vitro batch culture technique is being increasingly used to study rumen fermentation, but the results are affected by several factors, being the source of the inoculum one of the most relevant. This work was conducted to assess the effects of different processing methods of ruminal contents on microbial populations in the obtained fluid, and its influence on fermentation parameters when the fluid was used as inoculum for in vitro incubations. Rumen contents were obtained from four rumen-fistulated sheep fed a 2:1 alfalfa hay:concentrate diet and subjected to the following treatments: SQ: squeezed through four layers of cheesecloth; FL: SQ treatment and further filtration through a 100-μm nylon cloth; STO: blended for three min at 230 rev min−1 in a Stomacher® and further filtrations as in SQ. Microbial populations’ abundance and bacterial diversity in the ruminal fluids were analysed by quantitative PCR (qPCR) and automated ribosomal intergenic spacer analysis (ARISA), respectively. Three forages (alfalfa hay, grass hay and barley straw) were incubated in vitro, either alone or mixed with concentrate (1:1), using each of the ruminal fluids as inoculum. There were no differences between SQ and FL methods in any of the microbial populations analysed, but STO increased the relative abundance of Fibrobacter succinogenes and Ruminococcus albus (P < 0.05) and decreased (P < 0.05) the concentration of protozoal DNA compared with SQ. Bacterial diversity was not affected (P > 0.05) by the processing methods. There were no interactions (P > 0.05) between the processing method and the characteristics of the substrates for any of the fermentation parameters analysed. Compared with SQ, the STO method resulted in greater (P < 0.05) methane production and ammonia-N concentrations in 8 h incubations. After 24 h of incubation, the use of STO inoculum increased (P < 0.05) methane production and dry matter degradability compared with SQ, with no differences in the rest of the parameters. No differences between SQ and FL methods were detected in any parameter. The results show that stomaching the rumen contents prior to inoculation of in vitro cultures modified some microbial populations, but had only subtle effects on fermentation parameters.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGL2011-22628UnspecifiedUnspecifiedEstudio de la microbiota en sistemas in vitro de simulación de la fermentación ruminal (cultivos no renovados, fermentadores rusitec y fermentadores de flujo continuo)

More information

Item ID: 46101
DC Identifier: http://oa.upm.es/46101/
OAI Identifier: oai:oa.upm.es:46101
DOI: 10.1016/j.anifeedsci.2016.07.017
Official URL: https://doi.org/10.1016/j.anifeedsci.2016.07.017
Deposited by: Memoria Investigacion
Deposited on: 02 Jun 2017 15:41
Last Modified: 20 Mar 2019 19:01
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM