Differential modulation of plant immune responses by diverse 2 members of the Pseudomonas savastanoi pv. savastanoi HopAF 3 type III effector family

Castañeda Ojeda, M. Pilar and Lopez Solanilla, Emilia and Ramos, Cayo (2017). Differential modulation of plant immune responses by diverse 2 members of the Pseudomonas savastanoi pv. savastanoi HopAF 3 type III effector family. "Molecular Plant Pathology", v. 18 (n. 5); pp. 625-634. ISSN 1464-6722. https://doi.org/10.1111/mpp.12420.

Description

Title: Differential modulation of plant immune responses by diverse 2 members of the Pseudomonas savastanoi pv. savastanoi HopAF 3 type III effector family
Author/s:
  • Castañeda Ojeda, M. Pilar
  • Lopez Solanilla, Emilia
  • Ramos, Cayo
Item Type: Article
Título de Revista/Publicación: Molecular Plant Pathology
Date: June 2017
Volume: 18
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The Pseudomonas savastanoi pv. savastanoi NCPPB 3335 type III secretion system (T3SS) effector repertoire includes 33 candidates, seven of which translocate into host cells and interfere with plant defences. The present study was performed to investigate the co-existence of both plasmid- and chromosomalencoded members of the HopAF effector family, HopAF1-1 and HopAF1-2, respectively, in the genome of NCPPB 3335. Here, we show that the HopAF1 paralogues are widely distributed in the Pseudomonas syringae complex, where HopAF1-1 is most similar to the homologues encoded by other P. syringae pathovars infecting woody hosts that belong to phylogroups 1 and 3. We show that the expression of both HopAF1-1 and HopAF-2 is transcriptionally dependent on HrpL and demonstrate their delivery into Nicotiana tabacum leaves. Although the heterologous delivery of either HopAF1-1 or HopAF1-2 significantly suppressed the production of defence-associated reactive oxygen species levels, only HopAF1-2 reduced the levels of callose deposition. Moreover, the expression of HopAF1-2 by functionally effectorless P. syringae pv. tomato DC3000D28E completely inhibited the hypersensitive response in tobacco and significantly increased the competitiveness of the strain in Nicotiana benthamiana. Despite their functional differences, subcellular localization studies reveal that green fluorescent protein (GFP) fusions to either HopAF1-1 or HopAF1-2 are targeted to the plasma membrane when they are expressed in plant cells, a process that is completely dependent on the integrity of their N-myristoylation motif. Our results further support the notion that highly similar T3SS effectors might differentially interact with diverse plant targets, even when they co-localize in the same cell compartment.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGL2012-32516UnspecifiedUnspecifiedUnspecified
Government of SpainAGL2015-63851UnspecifiedUnspecifiedUnspecified
Government of SpainAGL2014-53242-C2-1UnspecifiedUnspecifiedUnspecified

More information

Item ID: 46136
DC Identifier: http://oa.upm.es/46136/
OAI Identifier: oai:oa.upm.es:46136
DOI: 10.1111/mpp.12420
Official URL: http://onlinelibrary.wiley.com/doi/10.1111/mpp.12420/full
Deposited by: Memoria Investigacion
Deposited on: 05 Jun 2017 13:55
Last Modified: 13 Mar 2019 12:40
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