A Developmental Switch of Gene Expression in the Barley Seed Mediated by HvVP1 (Viviparous-1) and HvGAMYB Interactions

Abraham, Zamira and Iglesias Fernandez, Raquel and Martinez Muñoz, Manuel and Rubio Somoza, Ignacio and Diaz Rodriguez, Isabel and Carbonero Zalduegui, Pilar and Vicente Carbajosa, Jesus (2016). A Developmental Switch of Gene Expression in the Barley Seed Mediated by HvVP1 (Viviparous-1) and HvGAMYB Interactions. "Plant Physiology", v. 170 (n. 4); pp. 2146-2158. ISSN 0032-0889. https://doi.org/10.1104/pp.16.00092.

Description

Title: A Developmental Switch of Gene Expression in the Barley Seed Mediated by HvVP1 (Viviparous-1) and HvGAMYB Interactions
Author/s:
  • Abraham, Zamira
  • Iglesias Fernandez, Raquel
  • Martinez Muñoz, Manuel
  • Rubio Somoza, Ignacio
  • Diaz Rodriguez, Isabel
  • Carbonero Zalduegui, Pilar
  • Vicente Carbajosa, Jesus
Item Type: Article
Título de Revista/Publicación: Plant Physiology
Date: February 2016
ISSN: 0032-0889
Volume: 170
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The accumulation of storage compounds in the starchy endosperm of developing cereal seeds is highly regulated at the transcriptional level. These compounds, mainly starch and proteins, are hydrolyzed upon germination to allow seedling growth. The transcription factor HvGAMYB is a master activator both in the maturation phase of seed development and upon germination, acting in combination with other transcription factors. However, the precise mechanism controlling the switch from maturation to germination programs remains unclear. We report here the identification and molecular characterization of Hordeum vulgare VIVIPAROUS1 (HvVP1), orthologous to ABA-INSENSITIVE3 from Arabidopsis thaliana. HvVP1 transcripts accumulate in the endosperm and the embryo of developing seeds at early stages and in the embryo and aleurone of germinating seeds up to 24 h of imbibition. In transient expression assays, HvVP1 controls the activation of Hor2 and Amy6.4 promoters exerted by HvGAMYB. HvVP1 interacts with HvGAMYB in Saccharomyces cerevisiae and in the plant nuclei, hindering its interaction with other transcription factors involved in seed gene expression programs, like BPBF. Similarly, this interaction leads to a decrease in the DNA binding of HvGAMYB and the Barley Prolamine-Box binding Factor (BPBF) to their target sequences. Our results indicate that the HvVP1 expression pattern controls the full Hor2 expression activated by GAMYB and BPBF in the developing endosperm and the Amy6.4 activation in postgerminative reserve mobilization mediated by GAMYB. All these data demonstrate the participation of HvVP1 in antagonistic gene expression programs and support its central role as a gene expression switch during seed maturation and germination.

More information

Item ID: 46343
DC Identifier: http://oa.upm.es/46343/
OAI Identifier: oai:oa.upm.es:46343
DOI: 10.1104/pp.16.00092
Official URL: http://www.plantphysiol.org/content/170/4/2146.abstract?sid=957ee0b2-815b-43ff-b68e-b03ab06f594c
Deposited by: Memoria Investigacion
Deposited on: 05 Jun 2017 13:15
Last Modified: 04 Jun 2019 07:20
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