A Developmental Switch of Gene Expression in the Barley Seed Mediated by HvVP1 (Viviparous-1) and HvGAMYB Interactions

Abraham, Zamira; Iglesias Fernandez, Raquel; Martinez Muñoz, Manuel; Rubio Somoza, Ignacio; Diaz Rodriguez, Isabel; Carbonero Zalduegui, Pilar y Vicente Carbajosa, Jesus (2016). A Developmental Switch of Gene Expression in the Barley Seed Mediated by HvVP1 (Viviparous-1) and HvGAMYB Interactions. "Plant Physiology", v. 170 (n. 4); pp. 2146-2158. ISSN 0032-0889. https://doi.org/10.​1104/​pp.​16.​00092.

Descripción

Título: A Developmental Switch of Gene Expression in the Barley Seed Mediated by HvVP1 (Viviparous-1) and HvGAMYB Interactions
Autor/es:
  • Abraham, Zamira
  • Iglesias Fernandez, Raquel
  • Martinez Muñoz, Manuel
  • Rubio Somoza, Ignacio
  • Diaz Rodriguez, Isabel
  • Carbonero Zalduegui, Pilar
  • Vicente Carbajosa, Jesus
Tipo de Documento: Artículo
Título de Revista/Publicación: Plant Physiology
Fecha: Febrero 2016
Volumen: 170
Materias:
Escuela: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Departamento: Biotecnología - Biología Vegetal
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

The accumulation of storage compounds in the starchy endosperm of developing cereal seeds is highly regulated at the transcriptional level. These compounds, mainly starch and proteins, are hydrolyzed upon germination to allow seedling growth. The transcription factor HvGAMYB is a master activator both in the maturation phase of seed development and upon germination, acting in combination with other transcription factors. However, the precise mechanism controlling the switch from maturation to germination programs remains unclear. We report here the identification and molecular characterization of Hordeum vulgare VIVIPAROUS1 (HvVP1), orthologous to ABA-INSENSITIVE3 from Arabidopsis thaliana. HvVP1 transcripts accumulate in the endosperm and the embryo of developing seeds at early stages and in the embryo and aleurone of germinating seeds up to 24 h of imbibition. In transient expression assays, HvVP1 controls the activation of Hor2 and Amy6.4 promoters exerted by HvGAMYB. HvVP1 interacts with HvGAMYB in Saccharomyces cerevisiae and in the plant nuclei, hindering its interaction with other transcription factors involved in seed gene expression programs, like BPBF. Similarly, this interaction leads to a decrease in the DNA binding of HvGAMYB and the Barley Prolamine-Box binding Factor (BPBF) to their target sequences. Our results indicate that the HvVP1 expression pattern controls the full Hor2 expression activated by GAMYB and BPBF in the developing endosperm and the Amy6.4 activation in postgerminative reserve mobilization mediated by GAMYB. All these data demonstrate the participation of HvVP1 in antagonistic gene expression programs and support its central role as a gene expression switch during seed maturation and germination.

Más información

ID de Registro: 46343
Identificador DC: http://oa.upm.es/46343/
Identificador OAI: oai:oa.upm.es:46343
Identificador DOI: 10.​1104/​pp.​16.​00092
URL Oficial: http://www.plantphysiol.org/content/170/4/2146.abstract?sid=957ee0b2-815b-43ff-b68e-b03ab06f594c
Depositado por: Memoria Investigacion
Depositado el: 05 Jun 2017 13:15
Ultima Modificación: 05 Jun 2017 13:15
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