Citation
Arias Alvárez, M. and García García, R. M. and López Tello, J. and Nieto, K. and García Rebollar, Pilar and Gutiérrez Adán, A. and Lorenzo, P.L.
(2016).
Alpha-tocopherol affects gene expression patterns of rabbit cumulus complexes and reduces apoptosis rate during in vitro maturation.
In: "30th Annual Meeting of the Brazilian Embryo Technology Society", 09/09/2016-10/09/2016, Barcelona. p. 1.
Abstract
Oxidative stress compromises oocyte developmental competence during in vitro maturation (IVM). Antioxidants
such as vitamin E may avoid this imbalance. The aim of this study was to investigate the effect of a-Tocopherol
(α-TocOH) on the relative mRNA abundance of genes involved in cumulus expansion (GJA1, PTGS2), cell cycle
and viability (AKT1), cell cycle regulation and apoptosis (Tp53, CASP3) and antioxidant response (SOD2, GPX1,
CAT) in rabbit cumulus oocyte complexes (COCs) in vitro matured. The apoptosis index in cumulus cells (CCs) and
the hydrogen peroxide (H2O2) released by the COCs in maturation media were also assessed. For these purposes,
COCs from follicles ≥1mm were recovered, selected and in vitro-matured for 16h (38ºC, 5% CO2) in a medium
containing TCM-199 (Sigma, Madrid, Spain) with 0.3% bovine serum albumin (Sigma, Madrid, Spain) and 10
ng/mL Epidermal Growth Factor (EGF) (Sigma, Madrid, Spain) supplemented with 0, 100, 200 or 400 μM α-
TocOH (Sigma, Madrid, Spain), named as 0E, 100E, 200E and 400E groups, respectively
