Alpha-tocopherol affects gene expression patterns of rabbit cumulus complexes and reduces apoptosis rate during in vitro maturation

Arias Alvárez, M. and García García, R. M. and López Tello, J. and Nieto, K. and García Rebollar, Pilar and Gutiérrez Adán, A. and Lorenzo, P.L. (2016). Alpha-tocopherol affects gene expression patterns of rabbit cumulus complexes and reduces apoptosis rate during in vitro maturation. In: "30th Annual Meeting of the Brazilian Embryo Technology Society", 09/09/2016-10/09/2016, Barcelona. p. 1.

Description

Title: Alpha-tocopherol affects gene expression patterns of rabbit cumulus complexes and reduces apoptosis rate during in vitro maturation
Author/s:
  • Arias Alvárez, M.
  • García García, R. M.
  • López Tello, J.
  • Nieto, K.
  • García Rebollar, Pilar
  • Gutiérrez Adán, A.
  • Lorenzo, P.L.
Item Type: Presentation at Congress or Conference (Article)
Event Title: 30th Annual Meeting of the Brazilian Embryo Technology Society
Event Dates: 09/09/2016-10/09/2016
Event Location: Barcelona
Title of Book: 30th Annual Meeting of the Brazilian Embryo Technology Society
Date: 2016
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Producción Agraria
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (137kB) | Preview

Abstract

Oxidative stress compromises oocyte developmental competence during in vitro maturation (IVM). Antioxidants such as vitamin E may avoid this imbalance. The aim of this study was to investigate the effect of a-Tocopherol (α-TocOH) on the relative mRNA abundance of genes involved in cumulus expansion (GJA1, PTGS2), cell cycle and viability (AKT1), cell cycle regulation and apoptosis (Tp53, CASP3) and antioxidant response (SOD2, GPX1, CAT) in rabbit cumulus oocyte complexes (COCs) in vitro matured. The apoptosis index in cumulus cells (CCs) and the hydrogen peroxide (H2O2) released by the COCs in maturation media were also assessed. For these purposes, COCs from follicles ≥1mm were recovered, selected and in vitro-matured for 16h (38ºC, 5% CO2) in a medium containing TCM-199 (Sigma, Madrid, Spain) with 0.3% bovine serum albumin (Sigma, Madrid, Spain) and 10 ng/mL Epidermal Growth Factor (EGF) (Sigma, Madrid, Spain) supplemented with 0, 100, 200 or 400 μM α- TocOH (Sigma, Madrid, Spain), named as 0E, 100E, 200E and 400E groups, respectively

More information

Item ID: 46591
DC Identifier: http://oa.upm.es/46591/
OAI Identifier: oai:oa.upm.es:46591
Official URL: http://www.sbte.org.br/reuniao2016/en/
Deposited by: Memoria Investigacion
Deposited on: 20 Jun 2017 14:45
Last Modified: 20 Jun 2017 14:45
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM