Genomic and Transcriptomic Compilation of Chloroplast Ionic Transporters of Physcomitrella patens. Study of NHAD Transporters in Na+ and K+ Homeostasis

Ruíz-Lau, Nancy and Saez Somolinos, Ángela and Lanza Lucio, Monica and Benito Casado, Begoña (2017). Genomic and Transcriptomic Compilation of Chloroplast Ionic Transporters of Physcomitrella patens. Study of NHAD Transporters in Na+ and K+ Homeostasis. "Plant And Cell Physiology", v. 58 (n. 12); pp. 2166-2178. ISSN 0032-0781. https://doi.org/10.1093/pcp/pcx150.

Description

Title: Genomic and Transcriptomic Compilation of Chloroplast Ionic Transporters of Physcomitrella patens. Study of NHAD Transporters in Na+ and K+ Homeostasis
Author/s:
  • Ruíz-Lau, Nancy
  • Saez Somolinos, Ángela
  • Lanza Lucio, Monica
  • Benito Casado, Begoña
Item Type: Article
Título de Revista/Publicación: Plant And Cell Physiology
Date: December 2017
ISSN: 0032-0781
Volume: 58
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (3MB) | Preview

Abstract

K+ is widely used by plant cells, whereas Na+ can easily reach toxic levels during plant growth, which typically occurs in saline environments; however, the effects and functions in the chloroplast have been only roughly estimated. Traditionally, the occurrence of ionic fluxes across the chloroplast envelope or the thylakoid membranes has been mostly deduced from physiological measurements or from knowledge of chloroplast metabolism. However, many of the proteins involved in these fluxes have not yet been characterized. Based on genomic and RNA sequencing (RNA-seq) analyses, we present a comprehensive compilation of genes encoding putative ion transporters and channels expressed in the chloroplasts of the moss Physcomitrella patens, with a special emphasis on those related to Na+ and K+ fluxes. Based on the functional characterization of nhad mutants, we also discuss the putative role of NHAD transporters in Na+ homeostasis and osmoregulation of this organelle and the putative contribution of chloroplasts to salt tolerance in this moss. We demonstrate that NaCl does not affect the chloroplast functionality in Physcomitrella despite significantly modifying expression of ionic transporters and cellular morphology, specifically the chloroplast ultrastructure, revealing a high starch accumulation. Additionally, NHAD transporters apparently do not play any essential roles in salt tolerance.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGL2012-36174UnspecifiedUniversidad Politénica de MadridEntrada de NA+ en la raíz y tolerancia al NACL en las plantas: aplicación para su estudio de un análisis funcional y genético
Government of SpainAGL2016-80593-RUnspecifiedUnspecifiedEntrada de sodio en arroz en condiciones de salinidad. Efecto de la simbiosis con piriformospora en la respuesta de la planta

More information

Item ID: 49074
DC Identifier: http://oa.upm.es/49074/
OAI Identifier: oai:oa.upm.es:49074
DOI: 10.1093/pcp/pcx150
Official URL: https://academic.oup.com/pcp/article-abstract/58/12/2166/4318867?redirectedFrom=fulltext
Deposited by: Memoria Investigacion
Deposited on: 15 Jan 2018 13:28
Last Modified: 01 Jan 2019 23:30
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM