Genetic and biological characterization of Macrophomina phaseolina (Tassi) Goid. causing crown and root rot of strawberry

Sánchez, Soledad and Chamorro, Manuel and Henríquez, José Luis and Grez, Javiera and Diaz Rodriguez, Isabel and Santos, Berta de los and Gambardella, Marina (2017). Genetic and biological characterization of Macrophomina phaseolina (Tassi) Goid. causing crown and root rot of strawberry. "Chilean Journal of Agricultural Research", v. 77 (n. 4); pp. 325-331. ISSN 0718-5820. https://doi.org/10.4067/S0718-58392017000400325.

Description

Title: Genetic and biological characterization of Macrophomina phaseolina (Tassi) Goid. causing crown and root rot of strawberry
Author/s:
  • Sánchez, Soledad
  • Chamorro, Manuel
  • Henríquez, José Luis
  • Grez, Javiera
  • Diaz Rodriguez, Isabel
  • Santos, Berta de los
  • Gambardella, Marina
Item Type: Article
Título de Revista/Publicación: Chilean Journal of Agricultural Research
Date: October 2017
ISSN: 0718-5820
Volume: 77
Subjects:
Freetext Keywords: Charcoal rot; chlorate sensitivity; Fragaria ×ananassa; genetic diversity; microsatellite marker; mycelial growth; optimal temperature; SSR
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

In recent years, crown and root rot of strawberry ( Fragaria × ananassa Duchesne ex Rozier) caused by Macrophomina phaseolina (Tassi) Goid. has affected strawberry production areas worldwide, and in developed countries its emergency has been attributed to the replacement of methyl bromide. The disease was reported in strawberry crop in Chile in 2013, in fields without fumigation. The use of resistant cultivars rises as an alternative to the management of this disease. The objective of this study was to perform a biological and molecular characterization of isolates obtained from two growing regions in Chile and Spain. A total of 35 isolates were characterized for mycelial growth at different temperatures and for chlorate sensitivity. Seven simple sequence repeat loci were used for genetic characterization. Differences were found between Chilean and Spanish isolates in both characterizations. The optimal temperature for mycelial growth was lower in Chilean than in Spanish isolates (30 and 35 °C, respectively). Meanwhile, Chilean isolates were more sensitive to chlorate. In terms of genetic characterization, Polymorphism Information Content (PIC) ranged from 0.38 to 0.85, two main groups were identified, the first group included Spanish isolates and the second group corresponded to Chilean isolates, results were supported by a population structure analysis. This study determined clear differences between two populations of Chilean and Spanish M. phaseolina isolates as causal agent of crown and root rot of strawberry.

More information

Item ID: 50053
DC Identifier: http://oa.upm.es/50053/
OAI Identifier: oai:oa.upm.es:50053
DOI: 10.4067/S0718-58392017000400325
Official URL: http://www.chileanjar.cl/files/V77i4Y2017id4534.pdf
Deposited by: Memoria Investigacion
Deposited on: 01 Jun 2018 08:17
Last Modified: 01 Jun 2018 08:17
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