Vacuolar processing enzyme 4 contributes to maternal control of grain size in barley by executing programmed cell death in the pericarp

Radchuk, Volodymir and Tran, Van and Radchuk, Ruslana and Díaz Mendoza, María Mercedes and Weier, Diana and Fuchs, Joerg and Riewe, David and Hensel, Goetz and Kumlehn, J. and Munz, Eberhard and Heinzel, Nicolas and Rolletschek, Hardy and Martinez Muñoz, Manuel and Borisjuk, Ljudmilla (2018). Vacuolar processing enzyme 4 contributes to maternal control of grain size in barley by executing programmed cell death in the pericarp. "New Phytologist", v. 218 (n. 3); pp. 1127-1142. ISSN 1469-8137. https://doi.org/10.1111/nph.14729.

Description

Title: Vacuolar processing enzyme 4 contributes to maternal control of grain size in barley by executing programmed cell death in the pericarp
Author/s:
  • Radchuk, Volodymir
  • Tran, Van
  • Radchuk, Ruslana
  • Díaz Mendoza, María Mercedes
  • Weier, Diana
  • Fuchs, Joerg
  • Riewe, David
  • Hensel, Goetz
  • Kumlehn, J.
  • Munz, Eberhard
  • Heinzel, Nicolas
  • Rolletschek, Hardy
  • Martinez Muñoz, Manuel
  • Borisjuk, Ljudmilla
Item Type: Article
Título de Revista/Publicación: New Phytologist
Date: May 2018
Volume: 218
Subjects:
Freetext Keywords: barley; caspase-like activities; grain size; maternal control of seed size; pericarp; programmed cell death (PCD); vacuolar processing enzyme
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - Share

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Abstract

The angiosperm embryo and endosperm are limited in space because they grow inside maternal seed tissues. The elimination of cell layers of the maternal seed coat by programmed cell death (PCD) could provide space and nutrition to the filial organs. Using the barley (Hordeum vulgare L.) seed as a model, we elucidated the role of vacuolar processing enzyme 4 (VPE4) in cereals by using an RNAi approach and targeting the enzymatic properties of the recombinant protein. A comparative characterization of transgenic versus wild-type plants included transcriptional and metabolic profiling, flow cytometry, histology and nuclear magnetic imaging of grains. The recombinant VPE4 protein exhibited legumain and caspase-1 properties in vitro. Pericarp disintegration was delayed in the transgenic grains. Although the VPE4 gene and enzymatic activity was decreased in the early developing pericarp, storage capacity and the size of the endosperm and embryo were reduced in the mature VPE4-repressed grains. The persistence of the pericarp in the VPE4-affected grains constrains endosperm and embryo growth and leads to transcriptional reprogramming, perturbations in signalling and adjustments in metabolism. We conclude that VPE4 expression executes PCD in the pericarp, which is required for later endosperm filling, and argue for a role of PCD in maternal control of seed size in cereals.

More information

Item ID: 50054
DC Identifier: http://oa.upm.es/50054/
OAI Identifier: oai:oa.upm.es:50054
DOI: 10.1111/nph.14729
Official URL: https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.14729
Deposited by: Memoria Investigacion
Deposited on: 18 Apr 2018 08:13
Last Modified: 01 Jun 2019 22:30
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