Gravimetric biosensor based on a 1.3 GHz AlN shear-mode solidly mounted resonator

Miguel Ramos, Mario de and Díaz Durán, Bárbara and Escolano Moyano, José Miguel and Barba Gea, Mariano and Mirea, Teona and Olivares Roza, Jimena and Clement Lorenzo, Marta and Iborra Grau, Enrique (2016). Gravimetric biosensor based on a 1.3 GHz AlN shear-mode solidly mounted resonator. "Sensors and actuators B-Chemical", v. 239 ; pp. 1282-1288. ISSN 0925-4005. https://doi.org/10.1016/j.snb.2016.09.079.

Description

Title: Gravimetric biosensor based on a 1.3 GHz AlN shear-mode solidly mounted resonator
Author/s:
  • Miguel Ramos, Mario de
  • Díaz Durán, Bárbara
  • Escolano Moyano, José Miguel
  • Barba Gea, Mariano
  • Mirea, Teona
  • Olivares Roza, Jimena
  • Clement Lorenzo, Marta
  • Iborra Grau, Enrique
Item Type: Article
Título de Revista/Publicación: Sensors and actuators B-Chemical
Date: September 2016
ISSN: 0925-4005
Volume: 239
Subjects:
Freetext Keywords: ravimetric biosensor, Shear mode AlN resonators, Surface functionalization, Sensor sensitivity
Faculty: E.T.S.I. Telecomunicación (UPM)
Department: Ingeniería Electrónica
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

We investigate the performance of solidly mounted resonators based on Ir/tilted-AlN/Ir piezoelectric stacks as biosensors. These films are deposited by varying the pressure, the cathode power and the tem- perature of a two-step process based on depositing (00 • 2)-tilted AlN active layers over an (10 • 3)-oriented AlN seed layer. To minimize the influence of the temperature coefficient of frequency on the stability of the biosensor, we use insulating acoustic mirrors made of layers of SiO 2 and amorphous TaO x with non- /4 thicknesses, which enables to reduce the TCF to − 14 ppm/ ◦ C. The mass loading of the resonators with SiO 2 thin films results in a sensitivity of 1800 kHz/pg • cm 2 . Surface functionalization consists on the binding of silane groups on plasma oxidized SiO 2 surfaces. After a glutaraldehyde link, streptavidin is bonded to the surface to receive biotinylated receptors for several species. We test thrombin-binding aptamer (TBA29 against thrombin, and IgG antibody against immunoglobulin). The sensors response to species of different molecular weight like TBA-29 (9.75 kDa) or IgG antibody (150 kDa) is monitored. Finally, we assess the response of the biosensors to different thrombin concentrations (ranging from 4 nM to 270 nM) on surfaces functionalized with the TBA29 aptamer.

Funding Projects

TypeCodeAcronymLeaderTitle
FP7304814RAPTADIAGUnspecifiedRapid Aptamer based diagnostics for bacterial meningitis
Government of SpainMAT2013-45957-RUnspecifiedUnspecifiedMateriales avanzados para biosensores basados en resonadores piezoeléctricos combinados con nanotubos de carbono y grafeno

More information

Item ID: 50070
DC Identifier: http://oa.upm.es/50070/
OAI Identifier: oai:oa.upm.es:50070
DOI: 10.1016/j.snb.2016.09.079
Official URL: https://www.sciencedirect.com/science/article/pii/S0925400516314976?via%3Dihub
Deposited by: Memoria Investigacion
Deposited on: 21 Apr 2018 08:13
Last Modified: 25 Mar 2019 15:50
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