Selection of aptamers to Neisseria meningitidis and Streptococcus pneumoniae surface specific proteins and affinity assay using thin film AIN resonators

Escolano Moyano, José Miguel and Díaz Durán, Bárbara and Miguel Ramos, Mario de and Olivares Roza, Jimena and Geday, Morten Andreas and Iborra Grau, Enrique (2017). Selection of aptamers to Neisseria meningitidis and Streptococcus pneumoniae surface specific proteins and affinity assay using thin film AIN resonators. "Sensors and actuators B-Chemical" (n. 246); pp. 591-596. ISSN 0925-4005. https://doi.org/10.1016/j.snb.2017.02.098.

Description

Title: Selection of aptamers to Neisseria meningitidis and Streptococcus pneumoniae surface specific proteins and affinity assay using thin film AIN resonators
Author/s:
  • Escolano Moyano, José Miguel
  • Díaz Durán, Bárbara
  • Miguel Ramos, Mario de
  • Olivares Roza, Jimena
  • Geday, Morten Andreas
  • Iborra Grau, Enrique
Item Type: Article
Título de Revista/Publicación: Sensors and actuators B-Chemical
Date: 20 February 2017
Subjects:
Freetext Keywords: Meningitis, SOMAmer, AlN gravimetric biosensor, PavA, FHbp
Faculty: E.T.S.I. Telecomunicación (UPM)
Department: Ingeniería Electrónica
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The first steps in the development of an aptasensor for bacterial meningitis diagnosis in real time are described: slow off-rate modified aptamers (SOMAmer) selection, gravimetric sensor fabrication and functionalization of its active surface. SOMAmers polyclonal populations were generated to surface specific proteins PavA (S. pneumoniae) and FHbp (N. meningitidis) by systematic evolution of ligands by exponential enrichment method (SELEX). After eight rounds, they were tested by direct enzyme-linked oligonucleotide assays (ELONA) and by high frequency (1.4 GHz) gravimetric sensors based on thin film AlN resonators operating in shear mode. The sensing surface of the resonators was functionalized using a silane-glutaraldehyde based protocol and the binding of PavA and FHbp was measured in real time. The SOMAmers polyclonal populations showing the best ELONA results have been also tested using gravimetric sensors and the binding to the protein functionalized surface was measured in real time showing positive results. These first steps towards the development of an aptasensor for the targeted bacteria demonstrate the potential of the method for sensitive, rapid, and cost effective detection of bacterial meningitis.

Funding Projects

TypeCodeAcronymLeaderTitle
FP7304814RAPTADIAGUnspecifiedRapid Aptamer based diagnostics for bacterial meningitis
Government of SpainMAT2013-45957-RUnspecifiedUnspecifiedUnspecified

More information

Item ID: 50082
DC Identifier: http://oa.upm.es/50082/
OAI Identifier: oai:oa.upm.es:50082
DOI: 10.1016/j.snb.2017.02.098
Official URL: https://www.sciencedirect.com/science/article/pii/S0925400517303258?
Deposited by: Memoria Investigacion
Deposited on: 30 Apr 2018 15:44
Last Modified: 30 May 2019 09:55
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