Characterization of Enterococcus faecalis isolates by Chicken Embryo Lethality Assay and ERIC-PCR.

Blanco García, Ana Estefanía; Barz, Martin; Cavero, David; Icken, Wiebke; Sharifi, A. Reza; Voss, Matthias; Buxade Carbo, Carlos y Presisinger, Rudolf (2018). Characterization of Enterococcus faecalis isolates by Chicken Embryo Lethality Assay and ERIC-PCR.. "Avian Pathology", v. 47 (n. 1); pp. 23-32. ISSN 0307-9457. https://doi.org/10.1080/03079457.2017.1359404.

Descripción

Título: Characterization of Enterococcus faecalis isolates by Chicken Embryo Lethality Assay and ERIC-PCR.
Autor/es:
  • Blanco García, Ana Estefanía
  • Barz, Martin
  • Cavero, David
  • Icken, Wiebke
  • Sharifi, A. Reza
  • Voss, Matthias
  • Buxade Carbo, Carlos
  • Presisinger, Rudolf
Tipo de Documento: Artículo
Título de Revista/Publicación: Avian Pathology
Fecha: 31 Enero 2018
Volumen: 47
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Otro
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

Texto completo

[img]
Vista Previa
PDF (Document Portable Format) - Se necesita un visor de ficheros PDF, como GSview, Xpdf o Adobe Acrobat Reader
Descargar (1MB) | Vista Previa

Resumen

Enterococcus faecalis is the major causative agent of amyloid arthropathy in chickens. Given the difficulty of estimating the risk from field strains, the Embryo Lethality Assay (ELA) is proposed in this study as model to predict the virulence of 68 avian E. faecalis strains. Additionally, Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) was used to characterize the genetic diversity of the E. faecalis strains. The ELA was performed ten times with subsets of seven-eight E. faecalis strains each on a sample of 9987 eggs including control groups. Three to 24 colony-forming units were inoculated into the allantoic cavity of 10-day-old embryos. The embryonic mortality rate (EMR) was determined by means of candling the eggs over a period of seven days. The ELA was able to distinguish the virulence of the E. faecalis strains. 26 strains were considered as avirulent strains with an EMR of below 40%. Five strains were highly virulent with an EMR above 80%. The remaining 37 strains were classified as strains of moderate virulence causing an EMR between 40 and 80%. The highest EMR occurred three and four days post inoculation (p.i.). From the 4th day p.i. almost no embryonic mortality was observed. Therefore, the ELA could be optimized by reducing experiment duration to four days p.i.. ERIC-PCR did not cluster the strains according to its virulence, although ERIC banding patterns revealed a considerable genetic diversity. In conclusion, the ELA can be considered a reliable and useful tool to predict the virulence of avian E. faecalis strains.

Más información

ID de Registro: 50172
Identificador DC: http://oa.upm.es/50172/
Identificador OAI: oai:oa.upm.es:50172
Identificador DOI: 10.1080/03079457.2017.1359404
URL Oficial: https://www.tandfonline.com/doi/full/10.1080/03079457.2017.1359404
Depositado por: Memoria Investigacion
Depositado el: 19 Abr 2018 14:53
Ultima Modificación: 19 Abr 2018 14:53
  • InvestigaM
  • GEO_UP4
  • Open Access
  • Open Access
  • Sherpa-Romeo
    Compruebe si la revista anglosajona en la que ha publicado un artículo permite también su publicación en abierto.
  • Dulcinea
    Compruebe si la revista española en la que ha publicado un artículo permite también su publicación en abierto.
  • Recolecta
  • Observatorio I+D+i UPM
  • OpenCourseWare UPM