Characterization of Enterococcus faecalis isolates by Chicken Embryo Lethality Assay and ERIC-PCR.

Blanco García, Ana Estefanía and Barz, Martin and Cavero, David and Icken, Wiebke and Sharifi, A. Reza and Voss, Matthias and Buxade Carbo, Carlos and Presisinger, Rudolf (2018). Characterization of Enterococcus faecalis isolates by Chicken Embryo Lethality Assay and ERIC-PCR.. "Avian Pathology", v. 47 (n. 1); pp. 23-32. ISSN 0307-9457. https://doi.org/10.1080/03079457.2017.1359404.

Description

Title: Characterization of Enterococcus faecalis isolates by Chicken Embryo Lethality Assay and ERIC-PCR.
Author/s:
  • Blanco García, Ana Estefanía
  • Barz, Martin
  • Cavero, David
  • Icken, Wiebke
  • Sharifi, A. Reza
  • Voss, Matthias
  • Buxade Carbo, Carlos
  • Presisinger, Rudolf
Item Type: Article
Título de Revista/Publicación: Avian Pathology
Date: 31 January 2018
ISSN: 0307-9457
Volume: 47
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Enterococcus faecalis is the major causative agent of amyloid arthropathy in chickens. Given the difficulty of estimating the risk from field strains, the Embryo Lethality Assay (ELA) is proposed in this study as model to predict the virulence of 68 avian E. faecalis strains. Additionally, Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) was used to characterize the genetic diversity of the E. faecalis strains. The ELA was performed ten times with subsets of seven-eight E. faecalis strains each on a sample of 9987 eggs including control groups. Three to 24 colony-forming units were inoculated into the allantoic cavity of 10-day-old embryos. The embryonic mortality rate (EMR) was determined by means of candling the eggs over a period of seven days. The ELA was able to distinguish the virulence of the E. faecalis strains. 26 strains were considered as avirulent strains with an EMR of below 40%. Five strains were highly virulent with an EMR above 80%. The remaining 37 strains were classified as strains of moderate virulence causing an EMR between 40 and 80%. The highest EMR occurred three and four days post inoculation (p.i.). From the 4th day p.i. almost no embryonic mortality was observed. Therefore, the ELA could be optimized by reducing experiment duration to four days p.i.. ERIC-PCR did not cluster the strains according to its virulence, although ERIC banding patterns revealed a considerable genetic diversity. In conclusion, the ELA can be considered a reliable and useful tool to predict the virulence of avian E. faecalis strains.

More information

Item ID: 50172
DC Identifier: http://oa.upm.es/50172/
OAI Identifier: oai:oa.upm.es:50172
DOI: 10.1080/03079457.2017.1359404
Official URL: https://www.tandfonline.com/doi/full/10.1080/03079457.2017.1359404
Deposited by: Memoria Investigacion
Deposited on: 19 Apr 2018 14:53
Last Modified: 19 Apr 2018 14:53
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