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Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique
Citation
Laguna Heras, Maria Fe and Holgado Bolaños, Miguel and López Hernández, Ana and Santamaría Fernández, Beatriz and Lavín Hueros, Álvaro and Soria, Javier and Suarez, Tatiana and Bardina, Carlota and Jara, Mónica and Sanza Gutiérrez, Francisco Javier and Casquel Del Campo, Rafael (2015). Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique. "Sensors", v. 15 ; pp. 19819-19829. ISSN 1424-8220. https://doi.org/10.3390/s150819819.
Description
| Title: | Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique |
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| Author/s: |
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| Item Type: | Article |
| Título de Revista/Publicación: | Sensors |
| Date: | 2015 |
| ISSN: | 1424-8220 |
| Volume: | 15 |
| Subjects: | |
| Freetext Keywords: | photonic sensing cells BICELLs; biosensors; label-free immunoassay; dry eye biomarkers |
| Faculty: | E.T.S.I. Industriales (UPM) |
| Department: | Física Aplicada e Ingeniería de Materiales |
| Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
Abstract
The specificity and affinity of antibody-antigen interactions is a fundamental way to achieve reliable biosensing responses. Different proteins involved with dry eye dysfunction: ANXA1, ANXA11, CST4, PRDX5, PLAA and S100A6; were validated as biomarkers. In this work several antibodies were tested for ANXA1, ANXA11 and PRDX5 to select the best candidates for each biomarker. The results were obtained by using Biophotonic Sensing Cells (BICELLs) as an efficient methodology for label-free biosensing and compared with the Enzyme-Linked Immuno Sorbent Assay (ELISA) technique.
Funding Projects
| Type | Code | Acronym | Leader | Title |
|---|---|---|---|---|
| Government of Spain | IPT-2011-1429-010000 | INNBIOD | Unspecified | INNovación y desarrollo de sistemas portátiles de detección BIOlógica Óptica de alta eficiencia |
| Government of Spain | TEC2012-31145 | PLATON | Unspecified | Unspecified |
| FP7 | 614057 | ENVIGUARD | Unspecified | Development of a biosensor technology for environmental monitoring and disease prevention in aquaculture ensuring food safety |
More information
| Item ID: | 50265 |
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| DC Identifier: | http://oa.upm.es/50265/ |
| OAI Identifier: | oai:oa.upm.es:50265 |
| DOI: | 10.3390/s150819819 |
| Official URL: | http://www.mdpi.com/1424-8220/15/8/19819 |
| Deposited by: | Memoria Investigacion |
| Deposited on: | 04 Jun 2018 14:56 |
| Last Modified: | 10 Jun 2019 16:31 |
