Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique

Laguna Heras, Maria Fe and Holgado Bolaños, Miguel and López Hernández, Ana and Santamaría Fernández, Beatriz and Lavín Hueros, Álvaro and Soria, Javier and Suarez, Tatiana and Bardina, Carlota and Jara, Mónica and Sanza Gutiérrez, Francisco Javier and Casquel Del Campo, Rafael (2015). Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique. "Sensors", v. 15 ; pp. 19819-19829. ISSN 1424-8220. https://doi.org/10.3390/s150819819.

Description

Title: Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique
Author/s:
  • Laguna Heras, Maria Fe
  • Holgado Bolaños, Miguel
  • López Hernández, Ana
  • Santamaría Fernández, Beatriz
  • Lavín Hueros, Álvaro
  • Soria, Javier
  • Suarez, Tatiana
  • Bardina, Carlota
  • Jara, Mónica
  • Sanza Gutiérrez, Francisco Javier
  • Casquel Del Campo, Rafael
Item Type: Article
Título de Revista/Publicación: Sensors
Date: 2015
ISSN: 1424-8220
Volume: 15
Subjects:
Freetext Keywords: photonic sensing cells BICELLs; biosensors; label-free immunoassay; dry eye biomarkers
Faculty: E.T.S.I. Industriales (UPM)
Department: Física Aplicada e Ingeniería de Materiales
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The specificity and affinity of antibody-antigen interactions is a fundamental way to achieve reliable biosensing responses. Different proteins involved with dry eye dysfunction: ANXA1, ANXA11, CST4, PRDX5, PLAA and S100A6; were validated as biomarkers. In this work several antibodies were tested for ANXA1, ANXA11 and PRDX5 to select the best candidates for each biomarker. The results were obtained by using Biophotonic Sensing Cells (BICELLs) as an efficient methodology for label-free biosensing and compared with the Enzyme-Linked Immuno Sorbent Assay (ELISA) technique.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainIPT-2011-1429-010000INNBIODUnspecifiedINNovación y desarrollo de sistemas portátiles de detección BIOlógica Óptica de alta eficiencia
Government of SpainTEC2012-31145PLATONUnspecifiedUnspecified
FP7614057ENVIGUARDUnspecifiedDevelopment of a biosensor technology for environmental monitoring and disease prevention in aquaculture ensuring food safety

More information

Item ID: 50265
DC Identifier: http://oa.upm.es/50265/
OAI Identifier: oai:oa.upm.es:50265
DOI: 10.3390/s150819819
Official URL: http://www.mdpi.com/1424-8220/15/8/19819
Deposited by: Memoria Investigacion
Deposited on: 04 Jun 2018 14:56
Last Modified: 10 Jun 2019 16:31
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