T-cell epitopes of the major peach allergen, Pru p 3: Identification and differential T-cell response of peach-allergic and non-allergic subjects

Tordesillas Villuendas, Leticia; Cuesta-Herranz, Javier; Gonzalez-Muñoz, Miguel; Pacios, Luis F.; Compes, Esther; Garcia-Carrasco, Belen; Sánchez-Monge Laguna de Rins, Rosa; Salcedo Duran, Gabriel y Díaz Perales, Araceli (2009). T-cell epitopes of the major peach allergen, Pru p 3: Identification and differential T-cell response of peach-allergic and non-allergic subjects. "Molecular Immunology", v. 46 (n. 4); pp. 722-728. ISSN 0161-5890. https://doi.org/10.1016/j.molimm.2008.10.018.

Descripción

Título: T-cell epitopes of the major peach allergen, Pru p 3: Identification and differential T-cell response of peach-allergic and non-allergic subjects
Autor/es:
  • Tordesillas Villuendas, Leticia
  • Cuesta-Herranz, Javier
  • Gonzalez-Muñoz, Miguel
  • Pacios, Luis F.
  • Compes, Esther
  • Garcia-Carrasco, Belen
  • Sánchez-Monge Laguna de Rins, Rosa
  • Salcedo Duran, Gabriel
  • Díaz Perales, Araceli
Tipo de Documento: Artículo
Título de Revista/Publicación: Molecular Immunology
Fecha: Febrero 2009
Volumen: 46
Materias:
Palabras Clave Informales: Lipid transfer protein; T-cell epitope; Peptide immunotherapy; Plant food allergy; Peach; Pru p 3
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Lipid transfer proteins (LTPs), particularly peach Pru p 3, are the most relevant plant food allergens in the South of Europe, and, therefore, their allergic properties have been extensively studied. However, neither T-cell epitopes nor their effect on the patients’ T-cell response has been investigated in any member of the LTP panallergen family. The objective of the present study was to map the major T-cell epitopes of Pru p 3, as well as to evaluate their induced T-cell response in peach-allergic versus control subjects. Thus, peripheral blood mononuclear cells (PBMCs) from 18 peach-allergic patients and Pru p 3-specific T-cell lines (TCLs) from 9 of them were cultured with Pru p 3 and with a panel of 17 derived peptides (10-mer overlapping in 5 amino acids representing the full sequence of Pru p 3). Proliferation in 5-day assays was carried out via tritiated-thymidine incorporation, while IL4 and IFNγ production was assessed via sandwich enzyme-linked immunosorbent tests (ELISA) of TCL culture supernatants. The results were compared to those obtained from 10 non-peach allergic control volunteers. Two consecutive peptides showed the highest activation capacity. About 74% of PBMCs and TCLs recognized them, forming a single T-epitope: Pru p 365–80. Additionally, other specific T-cell epitopes were observed. Pru p 325–35 was detected by more than 60% of TCLs from peach-allergic patients, and Pru p 345–55 only activated PBMCs from control subjects. Interestingly, TCLs from patients were associated with a Th2-type, whereas control TCLs presented a Th1-type cytokine response. The major immunogenic T-cell epitope identified in Pru p 3, Pru p 365–80, is a good candidate to develop new vaccines for hypersensitivity reactions associated with LTP allergens from Rosaceae fruits.

Más información

ID de Registro: 5061
Identificador DC: http://oa.upm.es/5061/
Identificador OAI: oai:oa.upm.es:5061
Identificador DOI: 10.1016/j.molimm.2008.10.018
URL Oficial: http://www.sciencedirect.com/science/journal/01615890
Depositado por: Memoria Investigacion
Depositado el: 25 Nov 2010 09:55
Ultima Modificación: 20 Abr 2016 14:01
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